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Ameliorating Effect of Gardenia jasminoides Extract on Amyloid Beta Peptide-induced Neuronal Cell Deficit  

Choi, Soo Jung (Department of Food Technology, Korea University)
Kim, Mi-Jeong (Department of Food Technology, Korea University)
Heo, Ho Jin (Department of Food Science & Technology and Institute of Agriculture & Life Sciences, Gyeongsang National University)
Hong, Bumshik (Department of Food and Biotechnology, Korea University)
Cho, Hong Yon (Department of Food and Biotechnology, Korea University)
Kim, Young Jun (Department of Food and Biotechnology, Korea University)
Kim, Hye Kyung (Department of Food and Biotechnology, Hanseo University)
Lim, Seung-Taik (Department of Food Technology, Korea University)
Jun, Woo Jin (Department of Food and Nutrition, Chonnam University)
Kim, Eun-Ki (Department of Biological Engineering, Inha University)
Shin, Dong-Hoon (Department of Food and Biotechnology, Korea University)
Publication Information
Molecules and Cells / v.24, no.1, 2007 , pp. 113-118 More about this Journal
Abstract
The brains of Alzheimer's disease (AD) patients are characterized by large deposits of amyloid beta peptide ($A{\beta}$). $A{\beta}$ is known to increase free radical production in nerve cells, leading to cell death that is characterized by lipid peroxidation, free radical formation, protein oxidation, and DNA/RNA oxidation. In this study, we selected an extract of Gardenia jasminoides by screening, and investigated its ameliorating effects on $A{\beta}$-induced oxidative stress using PC12 cells. The effects of the extract were evaluated using the 2',7'-dichlorofluorescein diacetate (DCF-DA) assay and the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction assay. To find the active component, the ethanol extract was partitioned with hexane, chloroform, and ethyl acetate, respectively, and the active component was purified by silica-gel column chromatography and HPLC. The results suggested that Gardenia jasminoides extract can reduce the cytotoxicity of $A{\beta}$ in PC 12 cells, possibly by reducing oxidative stress.
Keywords
Alzheimer's Disease; Amyloid Beta; Gardenia jasminoides; Oxidative Stress; PC12 Cells;
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Times Cited By KSCI : 2  (Citation Analysis)
Times Cited By Web Of Science : 6  (Related Records In Web of Science)
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