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Methanol Extracts of Stewartia koreana Inhibit Cyclooxygenase-2 (COX-2) and Inducible Nitric Oxide Synthase (iNOS) Gene Expression by Blocking NF-κB Transactivation in LPS-activated RAW 264.7 Cells  

Lee, Tae Hoon (Graduate School of Biotechnology and Institute of Life Sciences and Resources, Kyung Hee University)
Kwak, Han Bok (Department of Cell and Developmental Biology, Seoul National University School of Dentistry)
Kim, Hong-Hee (Department of Cell and Developmental Biology, Seoul National University School of Dentistry)
Lee, Zang Hee (Department of Cell and Developmental Biology, Seoul National University School of Dentistry)
Chung, Dae Kyun (Graduate School of Biotechnology and Institute of Life Sciences and Resources, Kyung Hee University)
Baek, Nam-In (Graduate School of Biotechnology and Institute of Life Sciences and Resources, Kyung Hee University)
Kim, Jiyoung (Graduate School of Biotechnology and Institute of Life Sciences and Resources, Kyung Hee University)
Publication Information
Molecules and Cells / v.23, no.3, 2007 , pp. 398-404 More about this Journal
Abstract
Cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) are involved in various pathophysiological processes such as inflammation and carcinogenesis. In a search for inhibitors of COX-2 and iNOS production we found that extracts of Stewartia koreana strongly inhibited NO and $PGE_2$ production in LPS-treated macrophage RAW 264.7 cells. We have now shown that the mRNA and protein levels of iNOS and COX-2 are reduced by the Stewartia koreana extract (SKE). SKE inhibited expression of an NF-${\kappa}B$ reporter gene in response to LPS, and gel mobility shift assays revealed that SKE reduced NF-${\kappa}B$ DNA-binding activity. The extract also inhibited LPS-induced phosphorylation of $I{\kappa}B-{\alpha}$ and nuclear translocation of p65. Administration of the extract reduced the symptoms of arthritis in a collagen-induced arthritic mouse model. These results indicate that Stewartia extracts contain potentially useful agents for preventing and treating inflammatory diseases.
Keywords
Cyclooxygenase-2; Inflammation; Nitric Oxide Synthase; Nuclear factor ${\kappa}B$; Stewartia koreana;
Citations & Related Records
Times Cited By KSCI : 5  (Citation Analysis)
Times Cited By Web Of Science : 9  (Related Records In Web of Science)
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