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PMA Activates Stat3 in the Jak/Stat Pathway and Induces SOCS5 in Rat Brain Astrocytes  

Hwang, Mi-Na (Research Institute, National Cancer Center)
Kim, Kwang Soo (Department of Pharmacology, Ajou University School of Medicine)
Choi, Yo-Woo (Research Institute, National Cancer Center)
Jou, Ilo (Department of Pharmacology, Ajou University School of Medicine)
Yoon, Sungpil (Research Institute, National Cancer Center)
Publication Information
Molecules and Cells / v.23, no.1, 2007 , pp. 94-99 More about this Journal
Abstract
Suppressors of cytokine signaling (SOCS) family members are negative feedback regulators of the Jak/Stat pathway, which is an essential inflammatory signaling pathway. We investigated expression of eight members of the SOCS family in rat astrocytes, using two inflammatory stimulants, PMA and IFN-${\gamma}$. Only a few SOCS genes were induced by both stimulants, and we detected an increase in SOCS5 protein with PMA. PMA activated the Jnk, Erk, p38, and Jak/Stat signal pathways. In addition, it increased the level of activated-Stat3 resulting from tyrosine phosphorylation. A gel-shift assay showed that a protein in nuclear extracts from PMA-treated cells was able to bind to Stat binding elements. These results suggest that activated Stat3 binds to SOCS promoters and leads to their transcriptional induction.
Keywords
Astrocyte; IFN-${\gamma}$; Jak/Stat; PMA; SOCS; Stat3;
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