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Induction of Immunity Against Hepatitis B Virus Surface Antigen by Intranasal DNA Vaccination Using a Cationic Emulsion as a Mucosal Gene Carrier  

Kim, Tae Woo (Laboratory of Infection and Immunology, Graduate School of Medicine, Korea University)
Chung, Hesson (Biomedical Research Center, Korea Institute of Science and Technology)
Kwon, Ick Chan (Biomedical Research Center, Korea Institute of Science and Technology)
Sung, Ha Chin (Graduate School of Biotechnology, Korea University)
Kang, Tae Heung (Laboratory of Infection and Immunology, Graduate School of Medicine, Korea University)
Han, Hee Dong (Laboratory of Infection and Immunology, Graduate School of Medicine, Korea University)
Jeong, Seo Young (College of Pharmacy, Kyung Hee University)
Publication Information
Molecules and Cells / v.22, no.2, 2006 , pp. 175-181 More about this Journal
Abstract
Delivery of DNA vaccines to airway mucosa would be an ideal method for mucosal immunization. However, there have been few reports of a suitable gene delivery system. In this study we used a cationic emulsion to immunize mice via the intranasal route with pCMV-S coding for Hepatitis B virus surface antigen (HBsAg). Complexing pCMV-S with a cationic emulsion dramatically enhanced HBsAg expression in both nasal tissue and lung, and was associated with increases in the levels of HBs-specific Abs in serum and mucosal fluids, of cytotoxic T lymphocytes (CTL) in the spleen and cervical and iliac lymph nodes, and of delayed-type hypersensitivity (DTH) against HBsAg. In contrast, very weak humoral and cellular immunities were observed following immunization with naked DNA. In support of these observations, a higher proliferative response of spleenocytes was detected in the group immunized with the emulsion/pCMV-S complex than in the group immunized with naked pCMV-S. These findings may facilitate development of an emulsion-mediated gene vaccination technique for use against intracellular pathogens that invade mucosal surfaces.
Keywords
Cationic Lipid Emulsion; Hepatitis B Virus; Mucosal DNA Vaccine;
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