Browse > Article

Mouse Transthyretin-related Protein Is a Hydrolase which Degrades 5-Hydroxyisourate, the End Product of the Uricase Reaction  

Lee, Youra (Systemic Proteomics Research Center, Korea Research Institute of Bioscience and Biotechnology)
Park, Byoung Chul (Systemic Proteomics Research Center, Korea Research Institute of Bioscience and Biotechnology)
Lee, Do Hee (Systemic Proteomics Research Center, Korea Research Institute of Bioscience and Biotechnology)
Bae, Kwang-Hee (Systemic Proteomics Research Center, Korea Research Institute of Bioscience and Biotechnology)
Cho, Sayeon (College of Pharmacy, Chung-Ang University)
Lee, Choong Hwan (Division of Bioscience and Biotechnology, IBST, Konkuk University)
Lee, Jong Suk (Division of Bioscience and Biotechnology, IBST, Konkuk University)
Myung, Pyung Keun (College of Pharmacy, Chungnam National University)
Park, Sung Goo (Systemic Proteomics Research Center, Korea Research Institute of Bioscience and Biotechnology)
Publication Information
Molecules and Cells / v.22, no.2, 2006 , pp. 141-145 More about this Journal
Abstract
Uric acid is the end product of the purine degradation pathway in humans. It is catabolized to allantoin by urate oxidase or uricase (E.C. 1.7.3.3.) in most vertebrates except humans, some primates, birds, and certain species of reptiles. Here we provide evidence that mouse transthyretin-related protein facilitates the hydrolysis of 5-hydroxyisourate, the end product of the uricase reaction. Mutagenesis experiments showed that the residues that are absolutely conserved across the TRP family, including His11, Arg51, His102, and the C-terminal Tyr-Arg-Gly-Ser, may constitute the active site of mTRP. Based on these results, we propose that the transthyretin-related proteins present in diverse organisms are not functionally related to transthyretin but actually function as hydroxyisourate hydrolases.
Keywords
Hydroxyisourate; Transthyretin-related Proteins; Uric Acid; Uricase;
Citations & Related Records

Times Cited By Web Of Science : 11  (Related Records In Web of Science)
연도 인용수 순위
  • Reference
1 Bongaerts, G. P. A., Uitzetter, J., Brouns, R., and Vogels, G. D. (1978) Uricase of Bacillus fastidiosus: properties and regulation of synthesis. Biochem. Biophys. Acta 527, 348-358   DOI   ScienceOn
2 Kahn, K. and Tipton, P. A. (1997) Kinetic mechanism and cofactor content of soybean root noodle urate oxidase. Biochemistry 36, 4731-4738   DOI   ScienceOn
3 Lee, K. W. and Roush, A. H. (1964) Allantoinase assays and their application to yeast and soybean allantoinases. Arch. Biochem. Biophys. 108, 460-467   DOI   ScienceOn
4 Schultz, A. C., Nygaard, P., and Saxild, H. H. (2001) Functional analysis of 14 genes that constitute the purine catabolic pathway in Bacillus subtilis and evidence for a novel regulon controlled by the PucR transcription activator. J. Bacteriol. 183, 3293-3302   DOI   ScienceOn
5 Pitts, O. M., Priest, D. G., and Fish, W. W. (1974) Uricase. Subunit composition and resistance to denaturants. Biochemistry 13, 888-892   DOI   ScienceOn
6 Wu, X., Muzny, D. M., Lee, C. C., and Caskey, C. T. (1992) Two independent mutational events in the loss of urate oxidase during hominoid evolution. J. Mol. Evol. 34, 78-84   DOI
7 Eneqvist, T., Lundberg, E., Nilsson, L., Abagyan, R., and Sauer- Eriksson, A. E. (2003) The transthyretin-related protein family. Eur. J. Biochem. 270, 518-532   DOI   ScienceOn
8 Lee, Y., Lee, D. H., Kho, C. W., Lee, A. Y., Jang, M., et al. (2005) Transthyretin-related proteins function to facilitate the hydrolysis of 5-hydroxyisourate, the end product of the uricase reaction. FEBS Lett. 579, 4769-4774   DOI   ScienceOn
9 Ramazzina, I., Folli, C., Secchi, A., Berni, R., and Percudani, R. (2006) Completing the uric acid degradation pathway through phylogenetic comparison of whole genomes. Nat. Chem. Biol. 2, 144-148   DOI   ScienceOn
10 Sarma, A. D., Serfozo, P., Kahn, K., and Tipton, P. A. (1999) Identification and purification of hydroxyisourate hydrolase, a novel ureide-metabolizing enzyme. J. Biol. Chem. 274, 33863-33865   DOI
11 Wu, X., Lee, C. C., Muzny, D. M., and Caskey, C. T. (1989) Urate oxidase: primary structure and evolutionary implications. Proc. Natl. Acad. Sci. USA 86, 9412-9416
12 Kahn, K. and Tipton, P. A. (1998) Spectroscopic characterization of intermediates in the urate oxidase reaction. Biochemistry 37, 11651-11659   DOI   ScienceOn
13 Lee, H., Hur, C.-G., Oh, C. J., Kim, H. B., Park, S-Y., et al. (2004) Analysis of the root nodule-enhanced transcriptome in soybean. Mol. Cells 18, 53-62
14 Nygaard, P. (1983) Utilization of preformed purine bases and nucleotides; in Metabolism of Nucleotides, Nucleosides and Nucleobases in Microorganisms, Muchch-Petersen, A. (ed.), pp. 27-93, Academic Press, London, UK
15 Kahn, K., Serfozo, P., and Tipton, P. A. (1997) Identification of the true product of the urate oxidase reaction. J. Am. Chem. Soc. 119, 5435-5224   DOI   ScienceOn
16 Keilin, J. (1959) Biological significance of uric acid on guanine excretion. Biol. Rev. 34, 265-296