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Temporal and Spatial Expression of Homeotic Genes Is Important for Segment-specific Neuroblast 6-4 Lineage Formation in Drosophila  

Kang, Sun-Young (Department of Biology Education, Seoul National University)
Kim, Su-Na (Department of Biology Education, Seoul National University)
Kim, Sang Hee (Department of Chemistry, Konkuk University)
Jeon, Sang-Hak (Department of Biology Education, Seoul National University)
Publication Information
Molecules and Cells / v.21, no.3, 2006 , pp. 436-442 More about this Journal
Abstract
Different proliferation of neuroblast 6-4 (NB6-4) in the thorax and abdomen produces segmental specific expression pattern of several neuroblast marker genes. NB6-4 is divided to form four medialmost cell body glia (MM-CBG) per segment in thorax and two MM-CBG per segment in abdomen. As homeotic genes determine the identities of embryonic segments along the A/P axis, we investigated if temporal and specific expression of homeotic genes affects MM-CBG patterns in thorax and abdomen. A Ubx loss-of-function mutation was found to hardly affect MM-CBG formation, whereas abd-A and Abd-B caused the transformation of abdominal MM-CBG to their thoracic counterparts. On the other hand, gain-of-function mutants of Ubx, abd-A and Abd-B genes reduced the number of thoracic MM-CBG, indicating that thoracic MM-CBG resembled abdominal MM-CBG. However, mutations in Polycomb group (PcG) genes, which are negative transregulators of homeotic genes, did not cause the thoracic to abdominal MM-CBG pattern transformation although the number of MM-CBG in a few percent of embryos were partially reduced or abnormally patterned. Our results indicate that temporal and spatial expression of the homeotic genes is important to determine segmental-specificity of NB6-4 daughter cells along the anterior-posterior (A/P) axis.
Keywords
abd-A; Abd-B; MM-CBG; Neuroblast; PcG Genes; Ubx; *;
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Times Cited By Web Of Science : 4  (Related Records In Web of Science)
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