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Cleaved Amplified Polymorphic Sequence and Amplified Fragment Length Polymorphism Markers Linked to the Fertility Restorer Gene in Chili Pepper (Capsicum annuum L.)  

Kim, Dong Sun (Center for Plant Molecular Genetics and Breeding Research, Seoul National University)
Kim, Dong Hwan (Center for Plant Molecular Genetics and Breeding Research, Seoul National University)
Yoo, Jae Hyoung (Joongbu Breeding and Research Station, SeminisKorea)
Kim, Byung-Dong (Center for Plant Molecular Genetics and Breeding Research, Seoul National University)
Publication Information
Molecules and Cells / v.21, no.1, 2006 , pp. 135-140 More about this Journal
Abstract
Cytoplasmic male sterility (CMS) in plants, which is due to failure to produce functional pollen, is a maternally inherited trait. Specific nuclear genes that suppress CMS, termed fertility restorer (Rf) genes, have been identified in several plants. In this study, Rfl-inked molecular markers in pepper (Capsicum annuum L.) were detected by bulked segregant analysis of eight amplified fragment length polymorphisms (AFLPs). Only AFRF8 was successfully converted to a cleaved amplified polymorphic sequence (CAPS) marker. This was named AFRF8CAPS and genotype determination using it agreed with that obtained with the original AFRF8. A linkage map with a total size of 54.1 cM was constructed with AFRF8CAPS and the seven AFLP markers using the Kosambi function. The AFRF8CAPS marker was shown to be closest to Rf with a genetic distance of 1.8 cM. These markers will be useful for fast and reliable detection of restorer lines during $F_1$ hybrid seed production and breeding programs in pepper.
Keywords
Amplified Fragment Length Polymorphism (AFLP); Bulked Segregant Analysis (BSA); Capsicum annuum L.; Cleaved Amplified Polymorphic Sequence (CAPS); Cytoplasmic Male Sterility (CMS); Fertility Restorer (Rf) Gene;
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Times Cited By KSCI : 4  (Citation Analysis)
Times Cited By Web Of Science : 19  (Related Records In Web of Science)
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