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Characterization of Homologous Defective Interfering RNA during Persistent Infection of Vero Cells with Japanese Encephalitis Virus  

Yoon, Sung Wook (Department of Biology and Research Institute of Basic Sciences, Kyung Hee University)
Lee, Sang-Yong (Department of Biology and Research Institute of Basic Sciences, Kyung Hee University)
Won, Sung-Yong (Department of Biology and Research Institute of Basic Sciences, Kyung Hee University)
Park, Sun-Hee (Department of Biology and Research Institute of Basic Sciences, Kyung Hee University)
Park, Soo-Young (Department of Biology and Research Institute of Basic Sciences, Kyung Hee University)
Jeong, Yong Seok (Department of Biology and Research Institute of Basic Sciences, Kyung Hee University)
Publication Information
Molecules and Cells / v.21, no.1, 2006 , pp. 112-120 More about this Journal
Abstract
It has been suggested that defective interfering (DI) RNA contributes to the persistence of Japanese encephalitis virus (JEV). In this study, we characterized molecular and biological aspects of the DI RNA and its relation to viral persistence. We identified a homologous DI virus intimately associated with JEV persistence in Vero cells. The production of DI RNA during undiluted serial passages of JEV coincided with the appearance of cells refractory to acute infection with JEV. We also established a Vero cell clone with a persistent JEV infection in which the DI RNA coreplicated efficiently at the expense of helper virus. The infectious virus yield of the clone fluctuated during its growth depending upon the amount of DI RNA accumulated in the previous replication cycle. Identification of the corresponding negative-sense RNA of the DI RNA indicated that the DI RNA functioned as a replication unit. Most of the DI RNA molecules retained their open reading frames despite a large deletion, encompassing most of the prM, the entire E, and the 5' half of the NS1 gene. Taken together, these observations suggest that the generation of homologous DI RNA during successive JEV acute infections in Vero cells probably participates actively in persistent JEV infection.
Keywords
Defective Interfering RNA; Japanese Encephalitis Virus; Persistent Infection;
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