Browse > Article

Effect of the Urease Accessory Genes on Activation of the Helicobacter pylori Urease Apoprotein  

Park, Jeong-Uck (Department of Microbiology, Gyeongsang National University College of Medicine)
Song, Jae-Young (Department of Microbiology, Gyeongsang National University College of Medicine)
Kwon, Young-Cheol (Department of Microbiology, Gyeongsang National University College of Medicine)
Chung, Mi-Ja (Department of Microbiology, Gyeongsang National University College of Medicine)
Jun, Jin-Su (Department of Pediatrics, Gyeongsang National University College of Medicine)
Park, Jeong-Won (Department of Microbiology, Gyeongsang National University College of Medicine)
Park, Seung-Gyu (Department of Microbiology, Gyeongsang National University College of Medicine)
Hwang, Hyang-Ran (Department of Microbiology, Gyeongsang National University College of Medicine)
Choi, Sang-Haeng (Department of Microbiology, Gyeongsang National University College of Medicine)
Baik, Seung-Chul (Department of Microbiology, Gyeongsang National University College of Medicine)
Kang, Hyung-Lyun (Department of Microbiology, Gyeongsang National University College of Medicine)
Youn, Hee-Shang (Department of Pediatrics, Gyeongsang National University College of Medicine)
Lee, Woo-Kon (Department of Microbiology, Gyeongsang National University College of Medicine)
Cho, Myung-Je (Department of Microbiology, Gyeongsang National University College of Medicine)
Rhee, Kwang-Ho (Department of Microbiology, Gyeongsang National University College of Medicine)
Publication Information
Molecules and Cells / v.20, no.3, 2005 , pp. 371-377 More about this Journal
Abstract
The roles that accessory gene products play in activating the Helicobacter pylori urease apoprotein were examined. The activity of the urease apoprotein increased in the following order when it was expressed with the accessory genes: ureG < ureGH < ureFGH < ureEFGH < ureIEFGH. Moreover, stepwise additions of ureE and ureI to ureFGH significantly increased urease activity. Urease apoproteins coexpressed with ureFGH, ureEFGH, and ureIEFGH had similar low chymotrypsin susceptibilities. In vivo and in vitro activation studies showed that the cooperative effect of the accessory proteins involved processes in which the UreFGH complex, UreE, and UreI were implicated. Thus, the UreFGH complex may serve to alter the conformation of the apoprotein into one that is more competent to assemble a stable metallocenter, and that facilitates cooperative effects.
Keywords
Chymotrypsin Susceptibility; Conformation; Helicobacter pylori; Urease Accessory Gene;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
Times Cited By Web Of Science : 5  (Related Records In Web of Science)
연도 인용수 순위
1 Cho, M. J., Jeon, B. S., Park, J. W., Jung, T. S., Song, J. Y., et al. (2002) Identifying the major proteome components of Helicobacter pylori strain 26695. Electrophoresis 23, 1161-1173   DOI   ScienceOn
2 Clayton, C. L., Pallen, M. J., Kleanthous, H., Wren, B. W., and Tabaqchali, S. (1990) Nucleotide sequence of two genes from Helicobacter pylori encoding for urease subunits. Nucleic Acids Res. 18, 362   DOI
3 Cussac, V., Ferrero, R L., and Labigne, A. (1992) Expression of Helicobacter pylori urease genes in Escherichia coli grown under nitrogen-limiting conditions. J. Bacteriol. 174, 2466-2473
4 Hu, L. T. and Mobley, H. L. (1993) Expression of catalytically active recombinant Helicobacter pylori urease at wild-type levels in Escherichia coli. Infect. Immun. 61, 2563-2569
5 Mobley, H. L., Island, M. D., and Hausinger, R. P. (1995) Molecular biology of microbial ureases. Microbiol. Rev. 59, 451-480
6 Park, I. S. and Hausinger, R. P. (1995a) Requirement of carbon dioxide for in vitro assembly of the urease nickel metallocenter. Science 267, 1156-1158   DOI   ScienceOn
7 Rektorschek, M., Buhmann, A., Weeks, D., Schwan, D., Bensch, K. W., et al (2000) Acid resistance of Helicobacter pylori depends on the UreI membrane protein and an inner membrane proton barrier. Mol. Microbiol. 36, 141-152   DOI   ScienceOn
8 Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
9 Labigne, A., Cussac, V., and Courcoux, P. (1991) Shuttle cloning and nucleotide sequences of Helicobacter pylori genes responsible for urease activity. J. Bacteriol. 173, 1920-1931
10 Hu, L. T. and Mobley, H. L. (1990) Purification and N-terminal analysis of urease from Helicobacter pylori. Infect. Immun. 58, 992-998
11 Towbin, H., Staehelin, T., and Gordon, J. (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA 76, 4350-4354
12 Kim, J. I., Baik, S. C., Cho, M. J., Lee, W. K., and Rhee, K. H. (1991) Purification of the urease of Helicobacter pylori and production of monoclonal antibody to the urease of Helicobacter pylori. J. Korean Soc. Microbiol. 26, 531-540
13 Park, I. S. and Hausinger, R. P. (1995b) Evidence for the presence of urease apoprotein complexes containing UreD, UreF, and UreG in cells that are competent for in vivo enzyme activation. J. Bacteriol. 177, 1947-1951
14 Ercan, A. and Grossman, S. H. (2003) Proteolytic susceptibility of creatin kinase isozymes and arginine kinase. Biochem. Biophys. Res. Commun. 306, 1014-1018   DOI   ScienceOn
15 Kim, T.-H., Lee, J.-Y., Kang, B.-S., and Bae, Y.-S. (2005) In Vitro characterization of protein kinase CKII ${\beta}$ mutants defective in ${\beta}$-${\beta}$ dimerization. Mol. Cells 19, 124-130
16 Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685   DOI   ScienceOn
17 Mobley, H. L., Hu, L. T., and Foxal, P. A. (1991) Helicobacter pylori urease: properties and role in pathogenesis. Scand. J. Gastroenterol. Suppl. 187, 39-46
18 Shizuya, H., Birren, B., Kim, U. J., Mancino, V., Slepak, T., et al. (1992) Cloning and stable maintenance of 300-kilobasepair fragments of human DNA in Escherichia coli using an F-factor-based vector. Proc. Natl. Acad. Sci. USA 89, 8794-8797
19 Benoit, S. and Maier, R. J. (2003) Dependence of Helicobacter pylori urease activity on the nickel-sequestering ability of the UreE accessory protein. J. Bacteriol. 185, 4787-4795   DOI
20 Dunn, B. E., Campbell, G. P., Perez-Perez, G. I., and Blaser, M. J. (1990) Purification and characterization of urease from Helicobacter pylori. J. Biol. Chem. 265, 9464-9469
21 Moffatt, B. A. and Studier, F. W. (1987) T7 lysozyme inhibits transcription by T7 RNA polymerase. Cell 49, 221-227   DOI   ScienceOn
22 Kim, C.-H. (2003) A Salmonella typhimurium rfaE mutant recovers invasiveness for human epithelial cells when complemented by wild type rfaE (controlling biosynthesis of ADP-Lglycero- D-manno-heptose-containing lipopolysaccharide). Mol. Cells 15, 226-232
23 Weatherburn, M. W. (1967) Phenol-hypochlorite reaction for determination of ammonia. Anal. Chem. 39, 971-974   DOI
24 Lee, M. H., Pankratz, H. S., Wang, S., Scott, R. A., Finnegan, M. G., et al. (1993) Purification and characterization of Klebsiella aerogenes UreE protein: a nickel-binding protein that functions in urease metallocenter assembly. Protein Sci. 2, 1042-1052   DOI   ScienceOn
25 Lee, W. K., An, Y. S., Cho, M. J., Baik, S. C., Choi, Y. J., et al. (1998) Control mechanism for production and activation of Helicobacter pylori urease. J. Korean Soc. Microbiol. 33, 1-13
26 Voland, P., Weeks, D. L., Marcus, E. A., Prinz, C., Sachs, G., et al. (2003) Interactions among the seven Helicobacter pylori proteins encoded by the urease gene cluster. Am. J. Physiol. Gastrointest. Liver Physiol. 284, G96-106
27 Akada, J. K., Shirai, M., Takeuchi, H., Tsuda, M., and Nakazawa, T. (2000) Identification of the urease operon in Helicobacter pylori and its control by mRNA decay in response to pH. Mol. Microbiol. 36, 1071-1084   DOI   ScienceOn
28 Hazell, S. L., Lee, A., Brady, L., and Hennessy, W. (1986) Campylobacter pyloridis and gastritis: association with intercellular spaces and adaptation to an environment of mucus as important factors in colonization of the gastric epithelium. J. Infect. Dis. 153, 658-663   DOI   ScienceOn
29 Chang, A. C. and Cohen, S. N. (1978) Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. J. Bacteriol. 134, 1141-1156