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Characterization and Transcriptional Expression of the α-Expansin Gene Family in Rice  

Shin, Jun-Hye (National Research Laboratory of Plant Functional Genomics, Pohang University of Science and Technology Biotech Center, Division of Molecular and Life Sciences, Pohang University of Science and Technology)
Jeong, Dong-Hoon (National Research Laboratory of Plant Functional Genomics, Pohang University of Science and Technology Biotech Center, Division of Molecular and Life Sciences, Pohang University of Science and Technology)
Park, Min Chul (Department of Life Sciences, The Catholic University of Korea)
An, Gynheung (National Research Laboratory of Plant Functional Genomics, Pohang University of Science and Technology Biotech Center, Division of Molecular and Life Sciences, Pohang University of Science and Technology)
Publication Information
Molecules and Cells / v.20, no.2, 2005 , pp. 210-218 More about this Journal
Abstract
The rice genome contains at least 28 EXPA (${\alpha}$-expansin) genes. We have obtained near full-length cDNAs from the previously uncharacterized genes. Analysis of these newly identified clones together with the 12 identified earlier showed that the EXPA genes contain up to two introns and encode proteins of 240 to 291 amino acid residues. The EXPA proteins contain three conserved motifs: eight cysteine residues at the N-terminus, four tryptophan residues at the C-terminus, and a histidine-phenylalanine-aspartate motif in the central region. EXPA proteins could be divided into six groups based on their sequence similarity. Most were strongly induced in two-day-old seedlings and in the roots of one-week-old plants. However, only 14 genes were expressed in the aboveground organs, and their patterns were quite diverse. Transcript levels of EXPA7, 14, 15, 18, 21, and 29 were greater in stems, while EXPA2, 4, 5, 6, and 16 were highly expressed in both stem and sheath but not in leaf blade. EXPA1 is leaf blade-preferential, and EXP9 is leaf sheath-preferential. Most of the root-expressed genes were more strongly expressed in the dividing zone. However, the Group 2 EXPA genes were also strongly expressed in both mature and dividing zones, while EXPA9 was preferentially expressed in the elongation zone. Fourteen EXPA genes were expressed in developing panicles, with some being expressed during most developmental stages, others only as the panicles matured. These diverse expression patterns of EXPA genes suggest that in general they have distinct roles in plant growth and development.
Keywords
Actin; Development; Expression; Reproductive Stage; Vegetative Stage;
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1 Cho, H. T. and Kende, H. (1997b) Expansins and internodal growth of deepwater rice. Plant Physiol. 113, 1145-1151
2 Cho, H. T. and Kende, H. (1997c) Expression of expansin genes is correlated with growth in deepwater rice. Plant Cell 9, 1661-1671   DOI   ScienceOn
3 Choi, D., Lee, Y., Cho, H. T., and Kende, H. (2003) Regulation of expansin gene expression affects growth and development in transgenic rice plants. Plant Cell 14, 1386-1398
4 Cosgrove, D. J., Bedinger, P., and Durachko, D. M. (1997) Group l allergens of grass pollen as cell wall-loosening agents. Proc. Natl. Acad. Sci. USA 94, 6559-6564
5 Dong, X. (1998) SA, JA, ethylene and disease resistance in plants. Curr. Opin. Plant Biol. 1, 316-322   DOI   ScienceOn
6 Fleming, A. J., McQueen-Mason, S., Mandel, T., and Kuhlemeier, C. (1997) Induction of leaf primordia by the cell wall protein expansin. Science 276, 1415-1418   DOI   ScienceOn
7 Hiwasa, K., Rose, J. K. C., Nakano, R., Inaba, A., and Kubo, Y. (2003) Differential expression of seven ${alpha}$-expansin genes during growth and ripening of pear fruit. Physiol. Plant. 117, 564-572   DOI   ScienceOn
8 Huang, J., Takano, T., and Akita, S. (2000) Expression of ${\alpha}$- expansin genes in young seedlings of rice (Oryza sativa L.). Planta 211, 467-473   DOI
9 Lee, Y. and Kende, H. (2001) Expression of ${alpha}$-expansins is correlated with internodal elongation in deepwater rice. Plant Physiol. 127, 645-654   DOI
10 Lopez-Millan, A. F., Ellis, D. R., and Crusak, M. A. (2004) Identification and characterization of several new members of the zip family of metal ion transporters in Medicago truncatula. Plant Mol. Biol. 54, 583-596   DOI   ScienceOn
11 Okamoto, A. and Okamoto, H. (1995) Two proteins regulate the cell-wall extensibility and the yield threshold in glycerinated hollow cylinders of cowpea hypocotyl. Plant Cell Environ. 18, 827-830   DOI   ScienceOn
12 Pien, S., Wyrzykowska, J., McQueen-Mason, S., Smart, C., and Fleming, A. (2001) Local expression of expansin induces the entire process of leaf development and modifies leaf shape. Proc. Natl. Acad. Sci. USA 98, 11812-11817
13 Oh, C. J., Kim, H. B., and An, C. S. (2003) Molecular cloning and complementation analysis of nifV gene from Frankia EulK1 strain. Mol. Cells 15, 27-33
14 Reidy, B., McQueen-Mason, S., Nosberger, J., and Fleming, A. (2001) Differential expression of ${alpha}$- and ${beta}$-expansin genes in the elongating leaf of Festuca patensis. Plant Mol. Biol. 46, 491-504   DOI   ScienceOn
15 Hayashi, T., Wong, Y. S., and MacLachlan, G. (1984) Pea xyHayashi, T., Wong, Y. S., and MacLachlan, G. (1984) Pea xyloglucan and cellulose. 2. Hydrolysis by pea endo-1,4-${\beta}$- glucanases. Plant Physiol. 75, 605-610   DOI   ScienceOn
16 Rey, L., Imperial, J., Paracious, J. M., and Ruiz-Argueso, T. (1994) Purification of Rhizobium leguminosarum HypB, a nickel-binding protein required for hydrogenase synthesis. J. Bacteriol. 176, 6066-6073
17 Rose, A. B. and Last, R. L. (1997) Introns act post-transcriptionally to increase expression of the Arabidopsis thaliana tryptophan pathway gene PAT1. Plant J. 11, 455-464   DOI   ScienceOn
18 Vasil, V., Clancy, M., Ferl, R. J., Vasil, I., and Hannah, L. C. (1989) Increased gene expression by the first intron of maize Shrunken-1 locus in grass species. Plant Physiol. 91, 1575-1579   DOI   ScienceOn
19 Christensen, A. H., Sharrock, R. A., and Quail, P. H. (1992) Maize polyubiquitin genes: structure, thermal perturbation of expression and transcript splicing, and promoter activity following transfer to protoplasts by electroporation. Plant Mol. Biol. 18, 675-689   DOI
20 McQueen-Mason, S., Durachko, D. M., and Cosgrove, D. J. (1992) Two endogenous proteins that induce cell wall expansion in plants. Plant Cell 4, 1425-1433   DOI
21 Reinhardt, D., Wittwer, F., Mandel, T., and Kuhlemeier, C. (1998) Localized upregulation of a new expansin gene predicts the site of leaf formation in the tomato meristem. Plant Cell 10, 1427-1437   DOI
22 Pieterse, C. M. J. and van Loon, L. C. (1999) Salicylic acid independent plant defense pathways. Curr. Rev. Plant Biol. 4, 52-58
23 Hoshikawa, K. (1989) The Growing Rice Plant, 1st ed., pp. 39-41, Nobunkyo, Japen
24 Cosgrove, D. J. (2000) Loosening of plant cell walls by expansins. Nature 407, 321-326   DOI   ScienceOn
25 Cosgrove, D. J., Li, L. C., Cho, H. T., Hoffmann-Benning, S., Moore, R. C., et al. (2002) The growing world of expansins. Plant Cell Physiol. 43, 1436-1444   DOI   ScienceOn
26 Jeon, J. S., Lee, S., Jung, K., Jun, S. H., Kim, C., et al. (2000) Tissue-preferential expression of a rice ${alpha}$-tubulin gene, Os- TubA1, mediated by the first intron. Plant Physiol. 123, 1005-1014   DOI
27 McQueen-Mason, S. and Cosgrove, D. J. (1994) Disruption of hydrogen bonding between plant cell wall polymers by proteins that induce wall extension. Proc. Natl. Acad. Sci. USA 91, 6574-6578
28 McElroy, D., Zhang, W., Cao, J., and Wu, R. (1990) Isolation of an efficient actin promoter for use in rice transformation. Plant Cell 2, 163-171   DOI   ScienceOn
29 Wu, Y. and Cosgrove, D. J. (1999) Expression of expansin genes in drought-stressed maize roots. Part II: expression pattern of 14 expansins in well-watered maize plants. Abstract 352 of Annu. Meeting Amer. Soc. Plant Physiol. July 24-28
30 Murashige, T. and Skoog, F. (1962) A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiol. Plant 15, 473-497   DOI
31 Sterky, F., Regan, S., Karlsson, J., Hertzberg, M., Rohde, A., et al. (1998) Gene discovery in the wood-forming tissues of poplar: analysis of 5,692 expressed sequence tags. Proc. Natl. Acad. Sci. USA 95, 13330-13335
32 Fry, S. C., Smith, R. C., Renwick, K. F., Martin, D. J., Hodge, S. K., et al. (1992) Xyloglucan endotransglycosylase, a new wall-loosening enzyme: activity from plants. Biochem. J. 282, 821-828
33 Maas, C., Laufs, J., Grant, S., Korfhage, C., and Werr, W. (1991) The combination of a novel stimulatory element in the first exon of the maize Shrunken-1 gene with the following intron 1 enhances reporter gene expression up to 1000-fold. Plant Mol. Biol. 16, 199-207   DOI
34 Cho, H. T. and Kende, H. (1997a) Expansins in deepwater rice internodes. Plant Physiol. 113, 1137-1143   DOI   ScienceOn
35 Shcherban, T. Y., Shi, J., Durachko, D. M., Guiltinan, M. J., McQueen-Mason, S., et al. (1995) Molecular cloning and sequence analysis of expansins, a highly conserved, multigene family of proteins that mediated cell wall extension in plants. Proc. Natl. Acad. Sci. USA 92, 9245-9249
36 Reymond, P. and Farmer, E. E. (1998) Jasmonate and salicylate as global signals for defense gene expression. Curr. Opin. Plant Biol. 1, 404-411   DOI   ScienceOn
37 Brummell, D. A., Harpster, M. H., and Dunsmuir, P. (1999) Differential expression of expansin gene family members during growth and ripening of tomato fruit. Plant Mol. Biol. 39, 161-169   DOI   ScienceOn
38 Park, C. J., Park, C. B., Hong, S. S., Lee, H. S., Lee, S. Y., et al. (2000) Characterization and cDNA cloning of two glycineand histidine-rich antimicrobial peptides from the roots of shepherd's purse, Capsella bursa-pastoris. Plant Mol. Biol. 44, 187-197   DOI   ScienceOn
39 Civello, P. M., Powell, A. L. T., Sabehat, A., and Bennett, A. B. (1999) An expansin gene expressed in ripening strawberry fruit. Plant Physiol. 121, 1273-1279   DOI
40 Lee, Y. and Kende, H. (2002) Expression of ${alpha}$-expansin and expansin-like genes in deepwater rice. Plant Physiol. 130, 1396-1405   DOI   ScienceOn
41 Lee, Y., Choi, D., and Kende, H. (2001) Expansins: everexpanding numbers and functions. Curr. Opin. Plant Biol. 4, 527-532   DOI   ScienceOn
42 Dempsey, D. A., Shah, J., and Klessig, D. F. (1999) Salicylic acid and disease resistance in plants. Crit. Rev. Plant Sci. 18, 547-575
43 Rose, J. K. C., Lee, H. H., and Bennett, A. B. (1997) Expression of a divergent expansin gene is fruit-specific and ripeningregulated. Proc. Natl. Acad. Sci. USA 94, 5955-5960
44 Harrison, E. P., McQueen-Mason, S., and Manning, K. (2001) Expression of six expansin genes in relation to extension activity in developing strawberry fruit. J. Exp. Biol. 52, 1437-1446
45 Xiong, L., Schumaker, K. S., and Zhu, J. K. (2002) Cell signaling during cold, drought, and salt stress. Plant Cell 14, S165-S183   DOI
46 Kende, H., Bradford, K. J., Brummell, D. A., Cho, H. T., Cosgrove, D. J., et al. (2004) Nomenclature for members of the expansin superfamily of genes and proteins. Plant Mol. Biol. 55, 311-314   DOI   ScienceOn
47 Callis, J., Fromm, M., and Walbot, V. (1987) Introns increase gene expression in cultured maize cells. Genes Dev. 1, 1183-1200   DOI
48 Luehrsen, K. R. and Walbot, V. (1991) Intron enhancement of gene expression and splicing efficiency of introns in maize cells. Mol. Gen. Genet. 225, 81-93