Browse > Article

Controlled Release of Cyclosporin A from Liposomes-in-Microspheres as an Oral Delivery System  

Park, Hee-Jung (Department of Material and Biochemical Engineering, Chonnam National University)
Lee, Chang-Moon (Department of Nuclear Medicine and Research Institute of Clinical Medicine, Chonbuk National University School of Medicine)
Lee, Yong-Bok (Department of Pharmacy, Chonnam National University)
Lee, Ki-Young (Faculty of Applied Chemical Engineering and The Research Institute for Catalysis, Chonnam National University,Center for Nano Fine Chemicals (post BK21 Program), Chonnam National University)
Publication Information
Biotechnology and Bioprocess Engineering:BBE / v.11, no.6, 2006 , pp. 526-529 More about this Journal
Abstract
The aim of this study was to prepare cyclosporin A-loaded liposome (CyA-Lip) as an oral delivery carrier, with their encapsulation into microspheres based on alginate or extracellular polysaccharide (EPS) p-m10356. The main advantage of liposomes in the microspheres (LIMs) is to improve the restricted drug release property from liposomes and their stability in the stomach environment. Alginate microspheres containing CyA-Lip were prepared with a spray nozzle; CyA-Liploaded EPS microspheres were also prepared using a w/o emulsion method. The shape of the LIMs was spherical and uniform, and the particle size of the alginate-LIMs ranged from 5 to $10\;{\mu}m$, and that of the EPS-LIMs was about $100\;{\mu}m$. In a release test, release rate of CyA in simulated intestinal fluid (SIF) from the LIMs was significantly enhanced compared to that in simulated gastric fluid (SGF). In addition, the CyA release rates were slower from formulations containing the liposomes compared to the microspheres without the liposome. Therefore, alginate-and EPS-LIMs have the potential for the controlled release of CyA and as an oral delivery system.
Keywords
cyclosporin; liposome; polysaccharide microsphere; intestinal lymphatics;
Citations & Related Records

Times Cited By Web Of Science : 7  (Related Records In Web of Science)
Times Cited By SCOPUS : 5
연도 인용수 순위
1 Lee, C. M., S. Lim, G. Y. Kim, D. Kim, D. W. Kim, H. C. Lee, and K. Y. Lee (2004) Rosin microparticles as drug carriers: influence of various solvents on the formation of particles and sustained-release of indomethacin. Biotechnol. Bioprocess Eng. 9: 476-481   DOI   ScienceOn
2 Feng, S. S., G. Ruan, and Q. T. Li (2004) Fabrication and characterization of a novel drug delivery device liposomesin- microsphere (LIM). Biomaterials 25: 5181-5189   DOI   ScienceOn
3 Rothkopf, C., A. Fahr, G. Fricker, G. L. Scherphof, and J. A. A. M. Kamps (2005) Uptake of phosphatidylserinecontaining liposomes by liver sinusoidal endothelial cells in the serum-free perfused rat liver. Biochim. Biophys. Acta 1668: 10-16   DOI   ScienceOn
4 Kim, H. J., C. M. Lee, Y. B. Lee, and K. Y. Lee (2005) Preparation and mucoadhesive test of CSA-loaded liposomes with different characteristics for the intestinal lymphatic delivery. Biotechnol. Bioprocess Eng. 10: 516-521   과학기술학회마을   DOI   ScienceOn
5 O'Driscoll, C. M. (2002) Lipid-based formulations for intestinal lymphatic delivery. Eur. J. Pharm. Sci. 15: 405-415   DOI   ScienceOn
6 Yoshikawa, H. (1997) Lymphatic delivery in 'rectal drug delivery systems'. Adv. Drug Deliv. Rev. 28: 239-251   DOI   ScienceOn
7 Crosasso, P., M. Ceruti, P. Brusa, S. Arpicco, F. Dosio, and L. Cattel (2000) Preparation, characterization and properties of sterically stabilized paclitaxel-containing liposomes. J. Control. Release 63: 19-30   DOI   ScienceOn
8 Murdan, S., T. Andrysek, and D. Son (2005) Novel gels and their dispersions-oral drug delivery systems for ciclosporin. Int. J. Pharm. 300: 113-124   DOI   ScienceOn
9 Porter, C. J. H. and W. N. Charman (1997) Uptake of drugs into the intestinal lymphatics after oral administration. Adv. Drug Deliv. Rev. 25: 71-89   DOI   ScienceOn
10 Ferreira Almeida, P. and A. J. Almeida (2004) Cross-linked alginate-gelatine beads: a new matrix for controlled release of pindolol. J. Control. Release 97: 431-439
11 Odeberg, J. M., P. Kaufmann, K. G. Kroon, and P. Hoglund (2003) Lipid drug delivery and rational formulation design for lipophilic drugs with low oral bioavailability, applied to cyclosporine. Eur. J. Pharm. Sci. 20: 375-382   DOI   ScienceOn
12 Lee, C. M., D. W. Kim, H. C. Lee, and K. Y. Lee (2004) Pectin microspheres for oral colon delivery: preparation using spray drying method and in vitro release of indomethacin. Biotechnol. Bioprocess Eng. 9: 191-195   DOI   ScienceOn
13 Charman, W. N. and C. J. H. Porter (1996) Lipophilic prodrugs designed for intestinal lymphatic transport. Adv. Drug Deliv. Rev. 19: 149-169   DOI   ScienceOn
14 Dai, C., B. Wang, H. Zhao, B. Li, and J. Wang (2006) Preparation and characterization of liposomes-in-alginate (LIA) for protein delivery system. Colloids Surf. B Biointerfaces 47: 205-210   DOI   ScienceOn
15 Lee, C. M., H. C. Lee, and K. Y. Lee (2005) O-palmitoylcurdlan sulfate (OPCurS)-coated liposomes for oral drug delivery. J. Biosci. Bioeng. 100: 255-259   DOI   ScienceOn
16 Moribe, K., E. Tanaka, K. Maruyama, and M. Iwatsuru (1998) Enhanced encapsulation of amphotericin B into liposomes by complex formation with polyethylene glycol derivatives. Pharm. Res. 15: 1737-1742   DOI   ScienceOn
17 Fielding, R. M. (1991) Liposomal drug delivery. Advantages and limitations from a clinical pharmacokinetic and therapeutic perspective. Clin. Pharmacokinet. 21: 155-164   DOI   ScienceOn
18 Iwanaga, K., S. Ono, K. Narioka, M. Kakemi, K. Morimoto, S. Yamashita, Y. Namba, and N. Oku (1999) Application of surface-coated liposomes for oral delivery of peptide: Effects of coating the liposome's surface on the GI transit of insulin. J. Pharm. Sci. 88: 248-252
19 Ribeiro, A. J., R. J. Neufeld, P. Arnaud, and J. C. Chaumeil (1999) Microencapsulation of lipophilic drugs in chitosancoated alginate microspheres. Int. J. Pharm. 187: 115-123   DOI   ScienceOn
20 Zheng, C. H., J. Q. Gao, Y. P. Zhang, and W. Q. Liang (2004) A protein delivery system: biodegradable alginatechitosan- poly(lactic-co-glycolic acid) composite microspheres. Biochem. Biophys. Res. Commun. 323: 1321- 1327   DOI   ScienceOn
21 Boinpally, R. R., S. L. Zhou, G. Devraj, P. K. Anne, S. Poondru, and B. R. Jasti (2004) Iontophoresis of lecithin vesicles of cyclosporin A. Int. J. Pharm. 274: 185-190   DOI   ScienceOn