Browse > Article

Application of Principal Component Analysis and Self-organizing Map to the Analysis of 2D Fluorescence Spectra and the Monitoring of Fermentation Processes  

Rhee, Jong-Il (School of Applied Chemical Engineering, Chonnam National University)
Kang, Tae-Hyoung (Department of Industrial Engineering, Chonnam National University)
Lee, Kum-Il (Department of Industrial Engineering, Chonnam National University)
Sohn, Ok-Jae (Laboratory of BioProcess Technology, Chonnam National University)
Kim, Sun-Yong (Laboratory of BioProcess Technology, Chonnam National University)
Chung, Sang-Wook (Department of Industrial Engineering, Chonnam National University)
Publication Information
Biotechnology and Bioprocess Engineering:BBE / v.11, no.5, 2006 , pp. 432-441 More about this Journal
Abstract
2D fluorescence sensors produce a great deal of spectral data during fermentation processes, which can be analyzed using a variety of statistical techniques. Principal component analysis (PCA) and a self-organizing map (SOM) were used to analyze these 2D fluorescence spectra and to extract useful information from them. PCA resulted in scores and loadings that were visualized in the score-loading plots and used to monitor various fermentation processes with recombinant Escherichia coli and Saccharomyces cerevisiae. The SOM was found to be a useful and interpretative method of classifying the entire gamut of 2D fluorescence spectra and of selecting some significant combinations of excitation and emission wavelengths. The results, including the normalized weights and variances, indicated that the SOM network is capable of being used to interpret the fermentation processes monitored by a 2D fluorescence sensor.
Keywords
fermentation processes; monitoring; principal component analysis; self-organizing map; 2D fluorescence sensor;
Citations & Related Records

Times Cited By Web Of Science : 4  (Related Records In Web of Science)
Times Cited By SCOPUS : 4
연도 인용수 순위
1 Sonnleitner, B. (2000) Instrumentation of biotechnologi-cal processes. pp. 1-64. In: K. Schugerl (ed.). Advances in Biochemical Engineering and Biotechnology. Springer, Berlin, Germany
2 Harms, P., Y. Rostov, and G. Rao (2002) Bioprocess monitoring. Curr. Opin. Biotechnol. 13: 124-127   DOI   ScienceOn
3 Schugerl, K., C. Lindemann, S. Marose, and T. Scheper (1998) Two-dimensional fluorescence spectroscopy for on-line bioprocess monitoring. pp. 1-27. Course Material for the Bioprocess Engineering Course. Supertar, Island of Brae, Croatia
4 Wolf, G., J. S. Almeida, C. Pinheiro, V. Correia, C. Rodri-gues, M. A. M. Reis, and J. G. Crespo (2001) Two-dimensional fluorometry coupled with artificial neural networks: a novel method for on-line monitoring of complex biological processes. Biotechnol. Bioeng. 72: 297-306   DOI   ScienceOn
5 Skibsted, E., C. Lindemann, C. Roca, and L. Olsson (2001) On-line bioprocess monitoring with a multi-wavelength fluorescence sensor using multivariate calibration. J. Biotechnol. 88: 47-57   DOI   ScienceOn
6 Jolliffe, I. T. (1986) Principal Component Analysis. Springer, New York, NY, USA
7 Bro, R. (2003) Multivariate calibration. What is in chemometrics for the analytical chemist? Anal. Chim. Acta 500: 185-194   DOI   ScienceOn
8 Guimet, F, J. Ferre, R. Boque, and F. X. Rius (2004) Application of unfold principal component analysis and parallel factor analysis to the extrapolatory analysis of olive oils by means of excitation-emission matrix fluorescence spectroscopy. Anal. Chim. Acta 515: 75-85   DOI   ScienceOn
9 Tartakovsky, B., L. A. Lishman, and R. L. Legge (1996) Application of multi-wavelength fluorometry for monitoring wastewater treatment process dynamics. Water Res. 30:2941-2948   DOI   ScienceOn
10 Dow, L. K., S. Kalelkar, and E. R. Dow (2004) Self-organizing maps for the analysis of NMR spectra. BioSilico 2: 157-163
11 Rhee, J. I., A. Ritzka, and T. Scheper (2004) On-line monitoring and control of substrate concentrations in biological processes by flow injection analysis systems. Biotechnol. Bioprocess Eng. 9: 156-165   DOI   ScienceOn
12 Lee, K.-I., Y.-S. Yim, S.-W. Chung, J. Wei, and J. I. Rhee (2005) Application of artificial neural networks to the analysis of two-dimensional fluorescence spectra in recombinant E. coli fermentation processes. J. Chem. Technol. Biotechnol. 80: 1036-1045   DOI   ScienceOn
13 Hantelmann, K., M. Kollecker, D. Hull, B. Hitzmann, and T. Scheper (2006) Two-dimensional fluorescence spectroscopy: a novel approach for controlling fed-batch cultivations. J. Biotechnol. 121: 410-417   DOI   ScienceOn
14 Marose, S., C. Lindemann, and T. Scheper (1998) Two-dimensional fluorescence spectroscopy: a new tool for online bioprocess monitoring. Biotechnol. Prog. 14: 63-74   DOI   ScienceOn
15 Cimander, C. and C. F. Mandenius (2002) Online monitoring of a bioprocess based on a multi-analyser system and multivariate statistical process modelling. J. Chem. Technol. Biotechnol. 77: 1157-1168   DOI   ScienceOn
16 Munoz de la Pena, A., N. Mora Diez, D. B. Gil, A. C. Olivieri, and G. M. Escandar (2006) Simultaneous determination of flufenamic and meclofenamic acids in human urine samples by second-order multivariate parallel factor analysis (PARAFAC) calibration of micellar-enhanced excitation-emission fluorescence data. Anal. Chim. Acta 569: 250-259   DOI   ScienceOn
17 Boehl, D., D. Solle, B. Hitzmann, and T. Scheper (2003) Chemometric modelling with two-dimensional fluorescence data for Claviceps purpurea bioprocess characterization. J. Biotechnol. 105: 179-188   DOI   ScienceOn
18 Haack, M. B., A. Eliasson, and L. Olsson (2004) On-line cell mass monitoring of Saccharomyces cerevisiae cultivations by multi-wavelength fluorescence. J. Biotechnol. 114: 199-208   DOI   ScienceOn
19 Kolehmainen, M., P. Ronkko, and O. Raatikainen (2003) Monitoring of yeast fermentation by ion mobility spectrometry measurement and data visualization with self-organizing maps. Anal. Chim. Acta 484: 93-100   DOI   ScienceOn
20 Chung, S.-Y., K.-H. Seo, and J. I. Rhee (2005) Influence of culture conditions on the production of extra-cellular 5-aminolevulinic acid (ALA) by recombinant E. coli. Process Biochem. 40: 385-394   DOI   ScienceOn
21 Teshima, N., H. Katsumate, M. Kurihara, T. Sakai, and T. Kawashima (1999) Flow-injection determination of copper (II) based on its catalysis on the redox reaction of cysteine with iron (III) in the presence of 1,10-phenanthroline. Talanta 50: 41-47   DOI   ScienceOn
22 Hisiger, S. and M. Jolicoeur (2005) A multiwavelength fluorescence probe: is one probe capable for on-line monitoring of recombinant protein production and biomass activity? J. Biotechnol. 117: 325-336   DOI   ScienceOn
23 Eliasson Lantz, A., P. forgensen, E. Poulsen, C. Lindemann, and L. Olsson (2006) Determination of cell mass and polymyxin using multi-wavelength fluorescence. J. Biotechnol. 121: 544-554   DOI   ScienceOn
24 Kim, J. E., E. J. Kim, W. J. Rhee, and T. H. Park (2005) Enhanced production of recombinant protein in Escherichia coli using silkworm hemolymph. Biotechnol. Bioprocess Eng. 10: 353-356   과학기술학회마을   DOI   ScienceOn
25 Tartakovsky, B., M. Scheintuch, J.-M. Hilmer, and T. Scheper (1996) Application of scanning fluorometry for monitoring of a fermentation process. Biotechnol. Prog. 12: 126-131   DOI   ScienceOn
26 Dufour, E. and A. Riaublanc (1997) Potentiality of spectroscopic methods for the characterization of dairy products. I. Front-face fluorescence study of raw, heated and homogenized milks. Lait 11: 657-670
27 Rhee, J. I., K.-I. Lee, C.-K. Kim, Y.-S. Yim, S.-W. Chung, J. Wei, and K.-H. Bellgardt (2005) Classification of two-dimensional fluorescence spectra using self-organizing maps. Biochem. Eng. J. 22: 135-144   DOI   ScienceOn
28 Tietze, F. (1969) Enzymic method for quantitative determination of nanogram amounts of total and oxidized glutathione: applications to mammalian blood and other tissues. Anal. Biochem. 27: 502-522   DOI   ScienceOn
29 Geladi, P., B. Sthson, J. Nystrom, T. Lillhinga, T. Lestander, and J. Burger (2004) Chemometrics in Spectroscopy. Spectrochim. Acta Part B 59: 1347-1357
30 Debeljak, Z., M. Strapac, and M. Medic-Saric (2001) Application of self-organizing maps for the classification of chromatographic systems and prediction of values of chro-matographic quantities. J. Chromatogr. A 925: 31-40   DOI   ScienceOn
31 Mukherjee, J., C. Lindermann, and T. Scheper (1999) Fluorescence monitoring during cultivation of Enterobac-ter aerogenes at different oxygen levels. Appl. Microbiol. Biotechnol. 52: 489-494   DOI
32 Hur, W and Y.-K. Chung (2005) On-line monitoring of IPTG induction for recombinant protein production using an automatic pH control signal. Biotechnol. Bioprocess Eng. 10: 304-308   과학기술학회마을   DOI   ScienceOn
33 Shimizu, H., K. Araki, S. Shioya, and K.-I. Suga (1991) Optimal production of glutathione by controlling the specific growth rate of yeast in fed-batch culture. Biotechnol. Bioeng. 38: 196-205   DOI