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Optimization of ${\gamma}-Polyglutamic$ Acid Production by Bacillus subtilis ZJU-7 Using a Surface-response Methodology  

Shi, Feng (Institute of Bioengineering, Department of Chemical Engineering and Bioengineering, Zhejiang University)
Xu, Zhinan (Institute of Bioengineering, Department of Chemical Engineering and Bioengineering, Zhejiang University)
Cen, Peilin (Institute of Bioengineering, Department of Chemical Engineering and Bioengineering, Zhejiang University)
Publication Information
Biotechnology and Bioprocess Engineering:BBE / v.11, no.3, 2006 , pp. 251-257 More about this Journal
Abstract
The components of the media used to elicit the biosynthesis of $poly-{\gamma}-glutamic$ acid $({\gamma}-PGA)$ by Bacillus subtilis ZJU-7 were investigated, particularly the carbon and nitrogen sources Of the 7 carbon sources investigated, sucrose induced the highest rate of ${\gamma}-PGA$ productivity; among the nitrogen sources, tryptone had the best effect for ${\gamma}-PGA$ production. A $2^{6-2}$ fractional factorial design was used to screen factors that influence ${\gamma}-PGA$ production significantly, and a central composite design was finally adopted to formulate the optimal medium. ${\gamma}-PGA$ productivity improved approximately 2-fold when the optimal medium was used compared with the original nonoptimized medium, and volumetric productivity reached a maximum of 58.2 g/L after a 24-h cultivation period.
Keywords
$poly-{\gamma}-glutamic$ acid; L-glutamic acid; Bacillus subtilis; response surface methodology;
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