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Recent Advances in DNA Sequencing by End-labeled Free-Solution Electrophoresis (ELFSE)  

Won, Jong-In (Department of Chemical Engineering, Hongik University)
Publication Information
Biotechnology and Bioprocess Engineering:BBE / v.11, no.3, 2006 , pp. 179-186 More about this Journal
Abstract
End-Labeled Free-Solution Electrophoresis (ELFSE) is a new technique that is a promising bioconjugate method for DNA sequencing (or separation) and genotyping by both capillary and microfluidic device electrophoresis. Because ELFSE enables high-resolution electrophoretic separation in aqueous buffer alone (i.e., without a polymer matrix), it eliminates the need to load viscous polymer networks into electrophoresis microchannels. To achieve microchannel DNA separations with high performance, ELFSE requires monodisperse perturbing entities (i.e., drag-tags), which create a large amount of frictional drag when pulled behind DNA during free-solution electrophoresis, and which have other properties suitable for microchannel electrophoresis. In this article, the theoretical concepts of ELFSE and the required characteristics of the drag-tag molecules for the ultimate performance of ELFSE are reviewed. Additionally, the merits and limitations of current drag-tags are also discussed in the context of recent experimental data of ELFSE separation (or sequencing).
Keywords
DNA sequencing; capillary electrophoresis (CE); microfluidic device; ELFSE; drag-tag; microchannel electrophoresis;
Citations & Related Records

Times Cited By Web Of Science : 4  (Related Records In Web of Science)
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