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Effect of Polymer Shielding on Elution of G3PDH Bound to Dye-ligand Adsorbent  

Ling Tau Chuan (Biochemical Recovery Group, Centre for Formulation Engineering, Department of Chemical Engineering, University of Birmingham, Department of Process and Food Engineering, Faculty of Engineering, Universiti Putra Malaysia)
Lyddiatt Andrew (Biochemical Recovery Group, Centre for Formulation Engineering, Department of Chemical Engineering, University of Birmingham, Millipore Biopharmaceutical Division, Bioprocessing Ltd)
Publication Information
Biotechnology and Bioprocess Engineering:BBE / v.11, no.1, 2006 , pp. 84-87 More about this Journal
Abstract
Batch binding experiments were performed to assess the recovery performance of glyceraldehyde 3-phosphate dehydrogenase (G3PDH) bound to the unshielded and polymer (polyvinyl pyrrolidone. PVP)-shielded dye-ligand (Cibacron Blue 3GA) adsorbent. The adoption of a polymer-shielded, dye-ligand technique facilitated the elution efficiency of bound G3PDH. It was demonstrated that the recovery of G3PDH using polymer-shielded dye-ligand adsorption yielded higher elution efficiency, at 60.5% and a specific activity of 42.3 IU/mg, after a low ionic strength elution (0.15 M NaCl). The unshielded dye-ligand yielded lower elution efficiency. at 6.5% and a specific activity of 10.2 IU/mg.
Keywords
dye-ligand; Cibacron Blue 3GA; G3PDH; elution yield; low ionic strength elution;
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