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Inhibition of Telomerase Activity in U937 Human Monocytic Leukemia Cells by Compound K, a Ginseng Saponin Metabolite  

Kang Kyoung-Ah (Department of Biochemistry, College of Medicine and Applied Radiological Science Research Institute, Cheju National University)
Lee Kyoung-Hwa (Department of Biochemistry, College of Medicine and Applied Radiological Science Research Institute, Cheju National University)
Chae Sung-Wook (Department of Biochemistry, College of Medicine and Applied Radiological Science Research Institute, Cheju National University)
Kim Jeong-Ki (Department of Biochemistry, College of Medicine and Applied Radiological Science Research Institute, Cheju National University)
Seo Jung-Yeon (Department of Biochemistry, College of Medicine and Applied Radiological Science Research Institute, Cheju National University)
Ham Yong-Ho (Laboratory of Molecular Oncology, Korea Institute of Radiological & Medical Sciences)
Lee Kee-Ho (Laboratory of Molecular Oncology, Korea Institute of Radiological & Medical Sciences)
Kim Bum-Joon (Department of Microbiology and Cancer Research Institute, College of Medicine, Seoul National University)
Kim Hee-Sun (Department of Neuroscience, College of Medicine, Ewha Womans University)
Kim Dong-Hyun (Department of Microbial Chemistry, College of Pharmacy, Kyung Hee University)
Hyun Jin Won (Department of Biochemistry, College of Medicine and Applied Radiological Science Research Institute, Cheju National University)
Publication Information
Biotechnology and Bioprocess Engineering:BBE / v.11, no.1, 2006 , pp. 7-12 More about this Journal
Abstract
Telomerase activation is detected in most cancerous cells; hence, telomerase is a highly selective target for cancer therapy, which plays an important role in the apoptotic process. We have previously reported that the ginseng saponin metabolite, Compound K (20-O-D-glucopyranosyl-20(S)-protopanaxadiol, IH901), inhibits cell proliferation by inducing apoptosis and cell cycle arrest at the $G_1$ phase. The present study investigated the regulation of telomerase activity in Compound K treated U937 cells. Compound K treatment caused a reduction in telomerase activity and down-regulated the human telomerase reverse transcriptase (hTERT) gene, resulting in the decreased expressions of its protein, and of the c-Myc and Spl proteins (transcription factors of hTERT). These results indicate that the anticancer activity of Compound K could be mediated by inhibition of the telomerase activity.
Keywords
Compound K; telomerase; human telomerase reverse transcriptase;
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