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Production of Extracellular Water Insoluble ${\beta}-1,3-Glucan$ (Curdlan) from Bacillus sp. SNC07  

Gummadi, Sathyanarayana N. (Department of Biotechnology, Indian Institute of Technology-Madras)
Kumar, Kislay (Department of Biotechnology, Indian Institute of Technology-Madras)
Publication Information
Biotechnology and Bioprocess Engineering:BBE / v.10, no.6, 2005 , pp. 546-551 More about this Journal
Abstract
[ ${\beta}-1,3-Glucan$ ] (curdlan) is a water-insoluble polysaccharide composed exclusively of ${\beta}-1,3\;linked$ glucose residues. Extracellular curdlan was mostly synthesized by Agrobacterium species and Alcaligenes faecalis under nitrogen-limiting conditions. In this study, we screened the microorganisms capable of producing extracellular curdlan from soil samples. For the first time, we reported Gram-positive bacterium Bacillus sp. SNC 107 capable of producing extracellular curdlan in appreciable amounts. The effect of different carbon sources on curdlan production was studied and found that the yield of curdlan was more when glucose was used as carbon source. It was also found that maximum production was achieved when the initial concentration of ammonium and phosphate in the medium was 0.5 and 1.9 g/L respectively. In this study the curdlan production was increased from 3 to 7g/L in shake flask cultures.
Keywords
extracellular polysaccharide; ${\beta}-1,3-glucan$; curdlan; Bacillus; nitrogen limitation;
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Times Cited By Web Of Science : 8  (Related Records In Web of Science)
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