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Identification and Characterization of Thermoplasma acidophilum 2-Keto-3-Deoxy-D-Gluconate Kinase: A New Class of Sugar Kinases  

Jung, Jin-Hwa (Department of Chemical Engineering and Division of Molecular Life and Sciences, Pohang University of Science and Technology)
Lee, Sun-Bok (Department of Chemical Engineering and Division of Molecular Life and Sciences, Pohang University of Science and Technology)
Publication Information
Biotechnology and Bioprocess Engineering:BBE / v.10, no.6, 2005 , pp. 535-539 More about this Journal
Abstract
The thermoacidophilic archaeon Thermoplasma acidophilum has long been known to utilize D-glucose via the non-phosphorylated Entner-Doudoroff (nED) pathway. We now report the identification of a gene encoding 2-keto-3-deoxy-D-gluconate (KDG) kinase. The discovery of this gene implies the presence of a glycolysis pathway, other than the nED pathway. It was found that Ta0122 in the T. acidophilum genome corresponded to KDG kinase. This enzyme shares no similarity with known KDG kinases, and belongs to a novel class of sugar kinases. Of the five sugars tested only KDG was utilized as a substrate.
Keywords
2-keto-3-deoxy-D-gluconate kinase; Thermoplasma acidophilum; glycolysis; non-phosphorylated Enter-Doudoroff pathway; phosphoprotein; Euryarchaeota;
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