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DNA Microarray Analysis of Immediate Response to EGF Treatment in Rat Schwannoma Cells  

OH, Min-Kyu (Department of Chemical and Biological Engineering, Korea University)
Scoles, Daniel R. (Rose Moss Neurogenetics Laboratory for Parkinson and Related Diseases, CSMS Burns and Allen Research Institute, Division of Neurology, Cedars-Sinai Medical Center)
Pulst, Stefan-M. (Rose Moss Neurogenetics Laboratory for Parkinson and Related Diseases, CSMS Burns and Allen Research Institute, Division of Neurology, Cedars-Sinai Medical Center)
Publication Information
Biotechnology and Bioprocess Engineering:BBE / v.10, no.5, 2005 , pp. 444-450 More about this Journal
Abstract
Epidermal growth factor (EGF) activates many intracellular effector molecules, which subsequently influence the expression levels of many genes involved in cell growth, apoptosis and signal transduction, etc. In this study, the early response of gene expressions due to EGF treatment was monitored using oligonucleotide DNA microarrays in rat schwannoma cell lines. An immunoblotting experiment showed the successful activation of EGF receptors and an effector protein, STAT5, due to EGF treatment. The microarray study showed that 35 genes were significantly induced and 2 were repressed within 60 min after the treatment. The list of induced genes included early growth response 1, suppressor of cytokine signaling 3, c-fos, interferon regulatory factor 1 and early growth response 2, etc. According to the microarray data, six of these were induced by more than 10-fold, and showed at least two different induction patterns, indicating complicated regulatory mechanisms in the EGF signal transduction.
Keywords
oligonucleotide microarray; expression profiling; immediate response; epidermal growth factor (EGF); schwannoma cell line;
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1 Schlessinger, J. (2000) Cell signaling by receptor tyrosine kinases. Cell 103: 211-225.   DOI   PUBMED   ScienceOn
2 Wiley, H. S., S. Y. Shvartsman, and D. A. Lauffenburger (2003) Computational modeling of the EGF-receptor system: a paradigm for systems biology. Trends Cell Biol. 13: 43-50   DOI   ScienceOn
3 Chen, B. K. and W. C. Chang (2000) Functional interaction between c-Jun and promoter factor Sp1 in epidermal growth factor-induced gene expression of human 12(S)-lipoxygenase. Proc. Natl. Acad. Sci. USA 97: 10406-10411
4 Wang, Y. N. and W. C. Chang (2003) Induction of disease- associated keratin 16 gene expression by epidermal growth factor is regulated through cooperation of transcription factors Sp1 and c-Jun. J. Biol. Chem. 278: 45848-45857.   DOI   ScienceOn
5 Taniguchi, T., K. Ogasawara, A. Takaoka, and N. Tanaka (2001) IRF family of transcription factors as regulators of host defense. Annu. Rev. Immunol. 19: 623-655   DOI   ScienceOn
6 Schlessinger, J. (2004) Common and distinct elements in cellular signaling via EGF and FGF receptors. Science 306: 1506-1507   DOI   PUBMED   ScienceOn
7 Khachigian, L. M. (2004) Early growth response-1: blocking angiogenesis by shooting the messenger. Cell Cycle 3: 10-11
8 Blagoev, B., S. E. Ong, I. Kratchmarova, and M. Mann (2004) Temporal analysis of phosphotyrosine-dependent signaling networks by quantitative proteomics. Nat. Biotechnol. 22: 1139-1145   DOI   ScienceOn
9 Irizarry, R. A., B. M. Bolstad, F. Collin, L. M. Cope, B. Hobbs, and T. P. Speed (2003) Summaries of Affymetrix GeneChip probe level data. Nucleic Acids Res. 31: e15   DOI   ScienceOn
10 Gutmann, D. H., A. C. Hirbe, and C. A. Haipek (2001) Functional analysis of neurofibromatosis 2 (NF2) missense mutations. Hum. Mol. Genet. 10: 1519-1529   DOI   ScienceOn
11 Parkinson, D. B., S. Dickinson, A. Bhaskaran, M. T. Kinsella, P. J. Brophy, D. L. Sherman, S. Sharghi-Namini, M. B. Duran Alonso, R. Mirsky, and K. R. Jessen (2003) Regulation of the myelin gene periaxin provides evidence for Krox-20-independent myelin-related signalling in Schwann cells. Mol. Cell. Neurosci. 23: 13-27   DOI   ScienceOn
12 Tonko-Geymayer, S., O. Goupille, M. Tonko, C. Soratroi, A. Yoshimura, C. Streuli, A. Ziemiecki, R. Kofler, and W. Doppler (2002) Regulation and function of the cytokineinducible SH-2 domain proteins, CIS and SOCS3, in mammary epithelial cells. Mol. Endocrinol. 16: 1680-1695   DOI   ScienceOn
13 Schadt, E. E., C. Li, C. Su, and W. H. Wong (2000) Analyzing high-density oligonucleotide gene expression array data. J. Cell. Biochem. 80: 192-202   DOI   ScienceOn
14 Farivar, R., S. Zangenehpour, and A. Chaudhuri (2004) Cellular-resolution activity mapping of the brain using immediate-early gene expression. Front. Biosci. 9: 104-109   DOI
15 Li, C. and W. H. Wong (2001) Model-based analysis of oligonucleotide arrays: expression index computation and outlier detection. Proc. Natl. Acad. Sci. USA 98: 31-36   DOI   ScienceOn
16 Mandl, M., D. N. Slack, and S. M. Keyse (2005) Specific inactivation and nuclear anchoring of extracellular signalregulated kinase 2 by the inducible dual-specificity protein phosphatase DUSP5. Mol. Cell. Biol. 25: 1830-1845   DOI   ScienceOn
17 Pawson, T. and P. Nash (2003) Assembly of cell regulatory systems through protein interaction domains. Science 300: 445-452   DOI   PUBMED   ScienceOn
18 Paukku, K. and O. Silvennoinen (2004) STATs as critical mediators of signal transduction and transcription: lessons learned from STAT5. Cytokine Growth Factor Rev. 15: 435-455   DOI   ScienceOn