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Screening of Exiguobacterium acetylicum from Soil Samples Showing Enantioselective and Alkalotolerant Esterase Activity  

Hwang Bum-Yeol (Institute for Molecular Biology and Genetics, and School of Chemical Engineering, Seoul National University)
Kim Ji-Hyun (Institute for Molecular Biology and Genetics, and School of Chemical Engineering, Seoul National University)
Kim Juhan (Institute for Molecular Biology and Genetics, and School of Chemical Engineering, Seoul National University)
Kim Byung-Gee (Institute for Molecular Biology and Genetics, and School of Chemical Engineering, Seoul National University)
Publication Information
Biotechnology and Bioprocess Engineering:BBE / v.10, no.4, 2005 , pp. 367-371 More about this Journal
Abstract
About 3,000 bacterial colonies with esterase activities were isolated from soil samples by enrichment culture and halo-size on Luria broth-tributyrin (LT) plates. The colonies were assayed for esterase activity in microtiter plates using enantiomerically pure (R)- and (S)-2-phenylbutyric acid resorufin ester (2PB-O-res) as substrates. Two enantioselective strains (JH2 and JH13) were selected by the ratio of initial rate of hydrolysis of enantiomerically pure (R)- and (S)-2-PB-O-res. When cell pellets were used, both strains showed high apparent enantioselectivity ($E_{app}>100$) for (R)-2PB-O-res and were identified as Exiguobacterium acetylicum. The JH13 strain showed high esterase activity on p-nitrophenyl acetate (pNPA), but showed low lipase activity on p-nitrophenyl palmitate (pNPP). The esterase was located in the soluble fraction of the cell extract. The crude intracellular enzyme preparation was stable at a pH range from 6.0 to 11.0.
Keywords
alkalotolerant; enantioselective; esterase; Exiguobacterium acetylicum; screening;
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