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Enhanced Production of Recombinant Protein in Escherichia coli Using Silkworm Hemolymph  

Kim Ji Eun (School of Chemical and Biological Engineering, Seoul National University)
Kim Eun Jeong (School of Chemical and Biological Engineering, Seoul National University)
Rhee Won Jong (School of Chemical and Biological Engineering, Seoul National University)
Park Tai Hyun (School of Chemical and Biological Engineering, Seoul National University)
Publication Information
Biotechnology and Bioprocess Engineering:BBE / v.10, no.4, 2005 , pp. 353-356 More about this Journal
Abstract
The effect of silkworm hemolymph on the expression of recombinant protein in Escherichia coli was investigated. The addition of silkworm hemolymph to the culture medium in­creased the production of recombinant $\beta$-galactosidase in E. coli. The production was dependent on the concentration of the added silkworm hemolymph, which increased 2-, 5-, and 8-fold in media supplemented with 1, 3, and $5\%$ silkworm hemolymph, respectively. To identify the effective component, the silkworm hemolymph was fractionated by gel filtration column chromatography. A fraction, with a molecular weight of about 30 K was identified as the effective component.
Keywords
E. coli; recombinant protein; silkworm hemolymph;
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Times Cited By Web Of Science : 14  (Related Records In Web of Science)
Times Cited By SCOPUS : 13
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