[KSCI] Korea Science Citation Index Service

Importance of the Degree of Antigen Polymerization by Detoxification in Modulating the Immunogenicity of Acellular Pertussis Vaccine

Bae Cheon-Soon (Vaccine Institute, GreenCross Vaccine Corp.,)
Hong Sung-Sang (Vaccine Institute, GreenCross Vaccine Corp.,)
Ahn Sang-Jeom (Vaccine Institute, GreenCross Vaccine Corp.,)
Jang Yang-Suk (Vaccine Institute, GreenCross Vaccine Corp.,)
Hur Byung-Ki (Department of Biotechnology and Bioengineering, Inha University)

Publication Information

Biotechnology and Bioprocess Engineering:BBE / v.10, no.3, 2005 , pp. 230-235 More about this Journal

Abstract

For the acellular pertussis vaccine with a high immunogenicity, the concentration, composition and characteristics of acellular pertussis antigens are the crucial points to be considered. Nevertheless, it has not been proved yet whether or not the polymerization degree, one of the characteristics of formalin-detoxified acellular pertussis antigens, has an influence on vaccine potency. Thus, in the present study, the correlations among detoxification conditions of acellular pertussis bulks, their polymerization degrees and their immunogenicities were examined. In addition, the relative importance of pertussis toxoid in vaccine immunogenicity was also investigated. Results show that a lower lysine concentration during detoxification induces highly-polymerized antigens, the immunogenicity has a great dependency on the polymerization degree of antigens, and also pertussis toxoid has a relatively stronger influence on the immunogenicity than other antigens. Accordingly, in the aspect of the potency of detoxified acellular pertussis vaccine, it can be demonstrated that the polymerization of antigens and its degree are the major factors affecting the immunogenicity along with a relatively high content of pertussis toxoid

Keywords

acellular pertussis vaccine; detoxification; immunogenicity; pertussis toxin; polymerization degree;

Citations & Related Records

Times Cited By KSCI : 2 (Citation Analysis)
Times Cited By Web Of Science : 1 (Related Records In Web of Science)
Times Cited By SCOPUS : 1

1	Cherry, J. D., J. Gornbein, U. Heininger, and K. Stehr (1998) A search for serologic correlates of immunity to Bordetella pertussis cough illnesses. Vaccine 16: 1901-1906 DOI ScienceOn
2	Guan, Y. X., H. X. Pan, Y. G. Gao, S. J. Yao, and M. G. Cho (2005) Refolding and purification of recombinant human interferon- $\gamma$ expressed as inclusion bodies in Escherichia coli using size exclusion chromatography. Biotechnol. Bioprocess Eng. 10: 122-127 DOI ScienceOn
3	Linggood, F. V., F. S. Muriel, A. J. Fulthorpe, A. J. Woiwod, and C. G. Pope (1962) The toxoiding of purified diphtheria toxin. Brit. J. exp. Path.14: 177-188
4	Scheibel, I. and C. P. Elo (1965) Irreversible detoxification of purified diphtheria toxin. Acta Pathol. Micrbiol. Scand. 65: 117-128 DOI
5	Goldsby, R. A., T. J. Kindt, and B. A. Osborne (2000) Kuby Immunology. 4th ed., W. H. Freeman and Company, NY, USA
6	Cooper, P. D. (1994) The selective induction of different immune responses by vaccine adjuvants. pp. 125-158. In: G. L. Ada (ed.). Strategies in Vaccine Design. Landes Company, Austin, USA
7	Gupta, R. K. and B. E. Rost (2000) Aluminum compounds as vaccine adjuvants. pp. 73-74. In: D. T. O'Hagan (ed.). Vaccine Adjuvants: Preparation Methods and Research Protocols. Humana Press, New Jersey, USA
8	Huet, M. E., E. Relyveld, and S. Camps (1992) Simplified activity evaluation of several tetanus vaccines. Biologicals 20: 35-43 DOI ScienceOn
9	Bae, C. S., G. Y. Lim, J. S. Kim, and B. K. Hur (2003) Quadrivalent combined vaccine, including diphtheria toxoid, tetanus toxoid, detoxified whole cell pertussis, and hepatitis B surface antigen. J. Microbiol. Biotechnol. 13: 338-343
10	Seiji, S. O., O. S. Tamotsu, K. Satoru, and M. Tyoku (1984) Effect of pH and lysine during detoxification of a hemorrhagic principle of HABU snake venom (Trimeresurus, Flavoviridis) with formalin on the immunogenicity of the toxoid. Japan J. Med. Sci. Biol. 37: 225-231 DOI
11	Gupta, R. K. and G. R. Siber (1995) Adjuvants for human vaccines-current status, problems and future prospects. Vaccine 13: 1263-1276 DOI ScienceOn
12	Yasuo, M. and T. Atsuko (1996) Pertussis vaccine. pp. 39- 51. In: O. Akira (ed.). Vaccine Handbook. Maruzen Publisher, Tokyo, Japan
13	Jenson, O. M. and C. Koch (1988) On the effect of Al(OH)3 as an immunological adjuvant. Acta Pathol. Microbiol. Immun. Scand. 96: 257-264 DOI
14	Edwards, K. M., M. D. Decker, and E. A. Mortimer Jr. (1999) Pertussis vaccine. pp. 293-344. In: S. A. Plotkin and W. A. Orenstein (eds.). Vaccines. W. B. Saunders company, Philadelphia, USA
15	Hewlett, E. L. and S. A. Halperin (1998) Serological correlates of immunity to Bordetella pertussis. Vaccine 16: 1899-1900 DOI ScienceOn
16	Lim, I. H., K. J. Lee, E. K. Lee, M. R. Choi, G. W. Lee, Y. Yoon, D. H. Park, and K. H. Jung (2004) High-yield purification and chracterization of recombinant human leukotactin- 1 in Pichia pastoris. Biotechnol. Bioprocess Eng. 9: 1-6 DOI ScienceOn
17	Relyveld, E. H., B. Bizzini, and R. K. Gupta (1998) Rational approaches to reduce adverse reactions in man to vaccines containing tetanus and diphtheria toxoids. Vaccine 16: 1016-1023 DOI ScienceOn
18	Global programme on vaccines (1996) State of the World's Vaccines and Immunization. World Health Organization, Geneva
19	Gupta, R. K., E. H. Relyveld, E. B. Lindbald, B. Bizzini, S. Benefraim, and C. K. Gupta (1993) Adjuvants: A balance between toxicity and adjuvanticity. Vaccine 11: 293-306 DOI ScienceOn
20	Shirodkar, S., R. L. Hutchinson, D. L. Perry, J. L. White, and S. L. Hem (1990) Aluminum compounds used as adjuvants in vaccines. Pharm. Res. 7: 1282-1288 DOI ScienceOn
21	Storsaeter, J., H. O. Hallander, L. Gustafsson, and P. Olin (1998) Levels of anti-pertussis antibodies related to protection after household exposure to Bordetella pertussis. Vaccine 16: 1907-1916 DOI ScienceOn
22	Bae, C. S., K. N. Park, S. J. Ahn, J. S. Kim, and B. K. Hur (2002) Novel formulation of quadrivalent combined vaccine including diphtheria toxoid, tetanus toxoid, acellular pertussis antigens and hepatitis B surface antigen. J. Microbiol. Biotechnol. 12: 787-792