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Polyethyleneimine Derivative for Nucleic Acid Model  

Lee, Chan-Woo (Department of Innovative Industrial Technology, Hoseo University)
Chae, Hee-Jeong (Department of Innovative Industrial Technology, Hoseo University, Department of Food and Biotechnology, Hoseo University)
Kwon, Young-Jin (Department of Disaster prevention and Technology)
Publication Information
Biotechnology and Bioprocess Engineering:BBE / v.10, no.3, 2005 , pp. 205-211 More about this Journal
Abstract
Water-soluble polyethyleneimine (PE) derivatives containing nucleic acid bases and hydrophilic amino acids such as homoserine (Hse) and serine were prepared by the activated ester method as nucleic acid models. From spectroscopic measurements, the polymers were found to interact with DNA accompanied by an induction of conformational change. Hypochromicity in UV spectra indicated that a stable polymer complex was formed between poly (A) with PEI­Hse-Ura by complementary hydrogen bonding with equimolar nucleic base units (adenine:uracil=1:1). The induced conformation of DNA by the interaction with the polymer containing uracil and homoserine (PEI-Hse-Ura) was concluded to be a super triple helical structure. The formation of the polymer complex, DNA: PEI-Hse-Ura, was found to be affected by the presence of metal ions such as $Ca^{2+}\;and\;Cu^{2+}$.
Keywords
nucleic acid model; polyethyleneimine; homoserine; interaction; metal ions;
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