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Purification During Crossflow Electromicrofiltration of Fermentation Broth  

Park Young G. (Prundle Envitech. LTD, Departmet of Chemical Engineering, Daejin University)
Publication Information
Biotechnology and Bioprocess Engineering:BBE / v.9, no.6, 2004 , pp. 500-505 More about this Journal
Abstract
The present study was to investigate the purification of a fermentation broth by an electromicrofiltration membrane. Microfiltration runs with a crude and a centrifuged broth, with solution of particles recovered from centrifugation and with permeates from microfiltration experiments were thus compared. Microfiltration performances were governed by colloids and small particles that induced sharp initial flux declines. For these results, the evolution of the overall membrane resistance was increased by $80\%$ in comparison with the electromicrofiltration membrane. The main focus of this study was set on the enhancement of the filtrate flux by an electric field. This pressure electrofiltration leads to a drastic improvement of the filtration by $100\%$ and the filtration time was thereby reduced. Pressure electrofiltration serves as an inter­esting alternative to the cross-flow filtration and it effectively separates advantageous constitu­ents such as amino acids and biopolymers from a fermentation broth. They were equally main­tained during the microelectrofiltration, although they were significantly reduced by $45\%$ by the microfiltration without the application of an electric field. Accordingly, since the electrofiltration membrane was provided more permeability, this study experimentally demonstrates that the permeability inside a membrane can be controlled using an electric field.
Keywords
fermentation; microfiltration; broth; centrifugation; electric field; electrofiltration;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
Times Cited By Web Of Science : 5  (Related Records In Web of Science)
Times Cited By SCOPUS : 5
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1 Engler, J. and M. Wiesner (1999) Particle fouling of a rotating membrane disk. Wat. Res. 34: 557-565   DOI   ScienceOn
2 Serra, C., M. Wiesner, and J. Laine (1999) Rotating membrane disk filters: Design evaluation using computational fluid dynamics. Chem. Eng. Sci. 72: 1-17   DOI
3 Holeschovsky, U. and C. Cooney (1991) Quantitative description of ultrafiltration in a rotating filtration device. AIChE J. 37: 1219-1226   DOI
4 Krstic, D., S. Markov, and M. Tekic (2001) Membrane fouling during cross-flow microfiltration of polyporous squamosus fermentation broth. Biochem. Eng. J. 9: 103-109   DOI   ScienceOn
5 Byers, C. and A. Amarnath (1995) Understand the potential of electro-separation. Chem. Eng. Progress Feburary: 63-69
6 Park, Y. (2000) Separation of $\Phi$X Hae III DNA with electrochromatography. Biotechnol. Bioprocess Eng. 5: 332-338   DOI   ScienceOn
7 Lararova, Z. and W. Serro (2002) Electromembrane separation of mineral suspensions: Influence of process parameters. Sep. Sci. Tech. 37: 515-534   DOI   ScienceOn
8 Galier, S. and H. Balmann (2001) Study of the mass transfer phenomena involved in an electrophoretic membrane contactor. J. Mem. Sci. 194: 117-133   DOI   ScienceOn
9 Park, Y. (2002) The effect of porosity of sieving particles on the removal efficiency of organic substances via biofilter in the fixed bed. Biotechnol. Bioprocess Eng. 7: 31-37   DOI   ScienceOn
10 Park, Y. (2003) Residence time distribution in the chro-matographic column: Applications in the separation engi-neering of DNA. Biotechnol. Bioprocess Eng. 8: 117-125   DOI   ScienceOn
11 Tanaka, T., Y. Yamagiwa, T. Nagano, M. Taniguchi, and K. Nakanishi (2001) Relationship between cake structure and membrane pore size in crossflow filtration of microbial cell suspension containing fine particles. J Chem. Eng. Japan 34: 1524-1531   DOI   ScienceOn
12 Park, Y. and H. Byun (2002) Application of the cake-filtration theory to analyze the permeate performance in poly(vinylbenzyl chloride)-filled microfiltration membrane. J. Ind. Eng. Chem. 8: 537-545