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Fabrication of Protein A-Viologen Hetero Langmuir- Blodgett Film for Fluorescence Immunoassay  

Lee, Woochang (Department of Chemical and Biomolecular Engineering, Sogang University)
Chun, Bum-Suk (Department of Chemical and Biomolecular Engineering, Sogang University)
Oh, Byung-Keun (Department of Chemical and Biomolecular Engineering, Sogang University)
Lee, Won-Hong (Department of Chemical and Biomolecular Engineering, Sogang University)
Park, Jeong-Woo (Department of Chemical and Biomolecular Engineering, Sogang University)
Publication Information
Biotechnology and Bioprocess Engineering:BBE / v.9, no.4, 2004 , pp. 241-244 More about this Journal
Abstract
Protein A molecular thin film was fabricated as a platform of antibody-based biosensor. For the immobilization of the protein A thin film, a viologen multilayer was built up using the Langmuir-Blodgett (LB) technique, and then, protein A was adsorbed on the viologen LB film by an electrostatic interaction force, which was formed as a hetero-film structure. For the deposition of viologen, surface pressure area ($\pi$-A) isotherm was investigated. The fabricated protein A-viologen hetero LB film was investigated using atomic force microscopy (AFM). Using the developed molecular film, antibody immobilization and fluorescence measurement was carried out.
Keywords
Langmuir-Blodgett film; viologen; protein A; antibody immobilization;
Citations & Related Records

Times Cited By Web Of Science : 5  (Related Records In Web of Science)
Times Cited By SCOPUS : 5
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