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Bioelectrocatalyzed Signal Amplification for Affinity Interactions at Chemically Modified Electrodes  

Hyun C. Yoon (Department of Biotechnology, Ajou University)
Kim, Hak-Sung (Department of Biological Sciences, Korea Advanced Institute of Science and Technology)
Publication Information
Biotechnology and Bioprocess Engineering:BBE / v.9, no.2, 2004 , pp. 107-111 More about this Journal
Abstract
A comparative study was performed to evaluate the signal amplification strategies in electrochemical affinity sensing, which included the direct electron transfer and diffusible-group mediated electron transfer between label enzymes that were specifically bound to target proteins and chemically modified electrode surfaces. As a platform surface for affinity recognition reactions, a double functionalized poly(amidoamine) dendrimer monolayer that was modified with ferrocene and biotin groups was constructed on a gold surface. With the chemically modified electrode, a model affinity sensing with avidin was investigated. The advantages of adopting the diffusible-group mediated signaling strategy were demonstrated in terms of signal sensitivity and stability.
Keywords
biosensor; immunosensor; electrochemical; avidin; affinity;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
Times Cited By Web Of Science : 9  (Related Records In Web of Science)
Times Cited By SCOPUS : 7
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