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Biocatalytic Preparation of Chiral Epichlorohydrins Using Recombinant Pichia pastoris Expressing Epoxide Hydrolase of Rhodotorula glutinis  

Kim, Hee-Sook (Department of Food Science and Technology, Kyungsung University)
Lee, Jae-Hwa (Department of Bioscience and Biotechnology, Silla University)
Park, Sunghoon (Department of Chemical and Biochemical Engineering, Pusan National University)
Lee, Eun-Yeol (Department of Food Science and Technology, Kyungsung University)
Publication Information
Biotechnology and Bioprocess Engineering:BBE / v.9, no.1, 2004 , pp. 62-64 More about this Journal
Abstract
The use of enantioselective hydrolysis for preparing chiral epichlorohydrins was investigated using recombinant Pichia pastoris with the enantioselective epoxide hydrolase of Rhodotorula glutinis. The rate of the recombinant epoxide hydrolase-catalyzed enantioselective hydrolysis of racemic epichlorohydrins was enhanced by the addition of 5%(v/v) Tween 20. Enantiopure (R)-epichlorohydrins with an enantiopurity of 100% ee and a yield of 26% were obtained within 5min from 50mM racemates.
Keywords
enantioselective hydrolysis; epichlorohydrin; epoxide hydrolase; Rhodotorula glutinis; Pichia pastoris;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
Times Cited By Web Of Science : 16  (Related Records In Web of Science)
Times Cited By SCOPUS : 14
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