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Partitioning of Recombinant Human Granulocyte-Macrophage Colony Stimulating Factor (hGM-CSF) from Plant Cell Suspension Culture in PEG/Sodium Phosphate Aqueous Two-phase Systems  

Lee, Jae-Hwa (Department of Bioscience and Biotechnology, Silla University)
Loc, Nguyen-Hoang (Faculty of Sciences, Hue University, Hue, Vietnam, Faculty of Biological Sciences, Chonbuk National University)
Kwon, Tae-Ho (Basic Science Research Institute, Chonbuk National University)
Yang, Moon-Sik (Faculty of Biological Sciences, Chonbuk National University)
Publication Information
Biotechnology and Bioprocess Engineering:BBE / v.9, no.1, 2004 , pp. 12-16 More about this Journal
Abstract
Partitioning of human granulocyte-macrophage colony stimulating factor (hGM-CSF) was achieved in the aqueous two-phase systems (ATPSs) using a crude extract of transgenic tobacco cell suspension culture. This study examined the effects of polyethylene glycol (PEG) molecular weight and concentration and the effects of sodium phosphate concentration in different PEG/sodium phosphate systems on the partition coefficient, K. The best ATPS system was 5% PEG 8,000/1.6 M sodium phosphate after 2 h of incubation at room temperature. In this system, hGM-CSF was partitioned in the PEG-rich phase with a yield of 57.99% and K$\_$hGM-CSF/ of 8.12. In another system, 3% PEG 10,000/1.6 M sodium phosphate, hGM-CSF was also partitioned primarily in the top phase with a yield of 45.66% and K$\_$hGM-CSF/ of 7.64 after 2 h of incubation at room temperature.
Keywords
aqueous two-phase system; hGM-CSF; purification; plant cell culture; recombinant protein;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
Times Cited By Web Of Science : 16  (Related Records In Web of Science)
Times Cited By SCOPUS : 13
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