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Enantioselective Hydrolysis of Racemic Styrene Oxide by Epoxide Hydrolase of Rhodosporidium kratochvilovae SYU-08  

Lee, Ji-Won (Department of Food Science and Technology, Kyungsung University)
Lee, Eun-Jung (Department of Food Science and Technology, Kyungsung University)
Yoo, Seung-Sik (Department of Chemical Engineering, Pusan National University)
Park, Sung-Hoon (Department of Chemical Engineering, Pusan National University)
Kim, Hee-Sook (Department of Food Science and Technology, Kyungsung University)
Lee, Eun-Yeol (Department of Food Science and Technology, Kyungsung University)
Publication Information
Biotechnology and Bioprocess Engineering:BBE / v.8, no.5, 2003 , pp. 306-308 More about this Journal
Abstract
Enantioselective hydrolysis for the production of chiral styrene oxide was investigated using the epoxide hydrolase activity of a newly isolated Rhodosporidium kratochvilovae SYU-08. The effects of reaction parameters - buffer type, pH, temperature, initial substrate concentrations, phenyl-1,2-ethanediol concentrations on hydrolysis rate, and enantioselectivity - were analyzed. Optically active (S)-styrene oxide with an enantiomeric excess higher than 99 % was obtained from its racemate. with a yield of 38 % (theoretically 50% maximum yield) from an initial concentration of 80 mM.
Keywords
enantioselective hydrolysis; epoxide hydrolase; Rhodosporidium kratochvilovae; chiral styrene oxide;
Citations & Related Records

Times Cited By Web Of Science : 8  (Related Records In Web of Science)
Times Cited By SCOPUS : 6
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