Browse > Article

Immobilization of Proteins on Magnetic Nanoparticles  

Wang, Tzu-Hsien (Department of Chemical Engineering, National Chung Cheng University)
Lee, Wen-Chien (Department of Chemical Engineering, National Chung Cheng University)
Publication Information
Biotechnology and Bioprocess Engineering:BBE / v.8, no.4, 2003 , pp. 263-267 More about this Journal
Abstract
Magnetic nanoparticles prepared from an alkaline solution of divalent and trivalent iron ions could covalently bind protein via the activation of Nethyl-N-(3-dimethylaminopropyl) carbodiimide (EDC). Trypsin and avidin were taken as the model proteins for the formation of protein-nanoparticle conjugates. The immobilized yield of protein increased with molar ratio of EDC/nanoparticie. Higher concentrations of added protein could yield higher immobilized protein densities on the particles. In contrast to EDC, the yields of protein immobilization via the a ctivation of cyanamide were relatively lower. Nanoparticles bound with avidin could attach a single-stranded DNA through the avidin-biotin interaction and hybridize with a DNA probe. The DNA hybridization was confirmed by fluorescence microscopy observations. Immobilized DNA on nanoparticles by this technique may have widespread applicability to the detection of specific nucleic acid sequence and targeting of DNA to particular cells.
Keywords
magnetic nanoparticle; protein immobilization; covalent binding; DNA hybridization;
Citations & Related Records

Times Cited By Web Of Science : 16  (Related Records In Web of Science)
Times Cited By SCOPUS : 14
연도 인용수 순위
1 Immobilization of protein and enzymes to fine magnetic particles /
[ Koneracka M;P.Kopcansky;M.Antalmk;M.Timko;C.N.Ramchand;D.Lobo;R.V.Mehta;R.V.Upadhyay ] / J.Magn.Magn.Mater   DOI
2 Assessment of methods for covalent binding of nucleic acids to magnetic beads,Dynabeads,and the characteris-tics of the bound nucleic acid in hybridization reactions /
[ Lund,V;R.Schmid;D.Rickwood;E.Hornes ] / Nucleic Acids Res   DOI   ScienceOn
3 Biological and biomedical aspects of magnetic fluid technology /
[ Roath,S ] / J.Magn.Magn.Mater   DOI
4 Immobilization of yeast alcohol dehydrigenase on magnetic nanoparticles for improving its stability /
[ Liao,M.-H;D.-H.Chen ] / Biotechnol.Lett   DOI   ScienceOn
5 Preparation and characterization of YADH-bound magnetic nanoparticles /
[ D.-H.Chen;M.-H.Liao ] / J.Mol.Catal.B:Enzymatic   DOI   ScienceOn
6 Direct binding of protein to magnetic particles /
[ Mehta R.V;R.V.Upadhyay;S.W.Charles;C.N.Ramchand ] / Biotechnol.Tech   DOI   ScienceOn
7 High-effciency intracellular magnetic labeling with novel superparamagnetic-tat peptide conjugates /
[ Josephson,L;C.-H.Tung;A.Moore;R.Weissleder ] / Bioconju-gate Chem   DOI   ScienceOn
8 Surface modification of superparamag-netic nanoparticles(Ferrofluid) studies with particle elec-trophoresis:Application to the specific targeting of cells /
[ Sestier C;M.F.Da-Silva;D.Sabolovic;J.Roger;J.N.Pons ] / Electrophoresis   DOI   ScienceOn
9 Amination of polystyrene mi-crowells:application to the covalent grafting of DNA probes for hybridization assays /
[ Zammatteo,N;C.Girardeaux;D.Delforge;J.J.Pireauz,J.Remacle ] / Anal.Biochem   DOI   ScienceOn
10 Preparation and properties of magnetite and poly-mer magnetite nanoparticles /
[ Dresco,P.A;V.S.Zaitsev;R.J.Gambino;B.Chu ] / Langmuir   DOI   ScienceOn
11 An-tibody-magnetite nanoparticles:In vitro characterization of a potential tumor-specific contrast agent for magnetic resonance imaging /
[ Tiefenauer,L.X;G.Kuhne;R.Y.Andres ] / Bioconjugate Chem   DOI   ScienceOn