In Vitro Combinatorial Mutagenesis of the 65th and 222nd Positions of the Green Fluorescent Protein of Aequarea victoria |
Nakano, Hideo
(Laboratory of Molecular Biotechnology, Graduate School of Biological and Agricultural Sciences, Nagoya University)
Okumura, Reiko (Laboratory of Molecular Biotechnology, Graduate School of Biological and Agricultural Sciences, Nagoya University) Goto, Chinatsu (Laboratory of Molecular Biotechnology, Graduate School of Biological and Agricultural Sciences, Nagoya University) Yamane, Tsuneo (Laboratory of Molecular Biotechnology, Graduate School of Biological and Agricultural Sciences, Nagoya University) |
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Efficient coupled transcription/translation for PCR template by a hollow fiber membrane bioreactor
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DOI ScienceOn |
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Modulator-mediated synthesis of active lipase of Pseudomonas sp. 109 by Escherichia coli cell-free coupled transcription/translation system
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DOI ScienceOn |
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In vitro method for the generation of protein libraries using PCR amplification of a single DNA molecule and coupled transcription/translation
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Green fluorescent protein
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In vitro analysis of roles of a disulfide bridge and a calcium binding site in activation of Pseudomonas sp. strain KWI-56 lipase
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DOI ScienceOn |
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Reduction of protein degradation by use of protease-deficient mutants in cell-free protein synthesis system of Escherichia coli
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DOI ScienceOn |
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Dosage effect of minor arginyl-and isoleucyl-tRNA on protein synthesis in an E.coli in vitro coulped transcription/translation system
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DOI ScienceOn |
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In vitro construction and screening of a Burkholderia cepacia lipase library using single-molecule PCR and cell-free protein synthesis
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DOI ScienceOn |
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Importance of disulfide bridge formation on folding of phospholipase D from Streptomyces antibioticus
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High-throughput, cloning-independent protein library construction by combining single-molecule DNA amplification in vitro expression
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Single-step single-molecule PCR of DNA with a homo-priming sequence using a single primer and hot-startable DNA polymerase
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DOI |
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Modifying the chain-length selectivity of the lipase from Burkholderia cepacia KWI-56 through in vitro combinatorial mutagenesis in the substrate binding site
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DOI |
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Functional antibody production using cell-free translation;Effects of protein disulfide isomerase and chaperones
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DOI ScienceOn |
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In vitro scanning saturation mutagenesis of an antibody binding pocket
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Expression of Fab fragment of catalytic antibody 6D9 in an Escherichia coli in vitro coulped transcription/translation system
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DOI |