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Cloning and Characterization of Mannheimia succiniciproducens MBEL55E Phosphoenolpyruvate Carboxykinase (pckA) Gene  

Lee, Sang-Yup (Metabolic and Biomolecular Engineering, National Research Laboratory, Department of Chemical & Biomolecular Engineering and BioProcess Engineering, Research Center, Korea Advanced Institute of Science and Technology)
Lee, Pyung-Cheon (Metabolic and Biomolecular Engineering, National Research Laboratory, Department of Chemical & Biomolecular Engineering and BioProcess Engineering, Research Center, Korea Advanced Institute of Science and Technology)
Hong, Soon-Ho (Metabolic and Biomolecular Engineering National Research Laboratory)
Chang, Ho-Nam (Department of Chemical & Biomoleculr Engineering and BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology)
Publication Information
Biotechnology and Bioprocess Engineering:BBE / v.7, no.2, 2002 , pp. 95-99 More about this Journal
Abstract
A pckA gene encoding phosphoenolpyruvate carboxykinase (PEPCK) was cloned and sequenced from the succinic acid producing bacterium Mannheimia succiniciproducens MBEL55E. The gene encoded a 538 residue polypeptide with a calculated molecular mass of 58.8 kDa and a calculated pI of 5.03. The deduced amino acid sequence of the M. succiniciprodutens MBEL55E PEPCK was similar to those of all known ATP-dependent PEPCKS.
Keywords
phosphoenolpyruvate carboxykinase; pckA; Mannheimia succiniciproducens MBEL55E;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
Times Cited By SCOPUS : 4
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