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http://dx.doi.org/10.3344/kjp.2019.32.3.160

The antinociceptive effect of artemisinin on the inflammatory pain and role of GABAergic and opioidergic systems  

Dehkordi, Faraz Mahdian (Department of Basic Sciences, Faculty of Veterinary Medicine, Shahrekord University)
Kaboutari, Jahangir (Department of Basic Sciences, Faculty of Veterinary Medicine, Shahrekord University)
Zendehdel, Morteza (Department of Physiology, Faculty of Veterinary Medicine, University of Tehran)
Javdani, Moosa (Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahrekord University)
Publication Information
The Korean Journal of Pain / v.32, no.3, 2019 , pp. 160-167 More about this Journal
Abstract
Background: Pain is a complex mechanism which involves different systems, including the opioidergic and GABAergic systems. Due to the side effects of chemical analgesic agents, attention toward natural agents have been increased. Artemisinin is an herbal compound with widespread modern and traditional therapeutic indications, which its interaction with the GABAergic system and antinoniceptive effects on neuropathic pain have shown. Therefore, this study was designed to evaluate the antinociceptive effects of artemisinin during inflammatory pain and interaction with the GABAergic and opioidergic systems by using a writhing response test. Methods: On the whole, 198 adult male albino mice were used in 4 experiments, including 9 groups (n = 6) each with three replicates, by intraperitoneal (i.p.) administration of artemisinin (2.5, 5, and 10 mg/kg), naloxone (2 mg/kg), bicuculline (2 mg/kg), saclofen (2 mg/kg), indomethacin (5 mg/kg), and ethanol (10 mL/kg). Writhing test responses were induced by i.p. injection of 10 mL/kg of 0.6% acetic acid, and the percentage of writhing inhibition was recorded. Results: Results showed significant dose dependent anti-nociceptive effects from artemisinin which, at a 10 mg/kg dose, was statistically similar to indomethacin. Neither saclofen nor naloxone had antinociceptive effects and did not antagonize antinociceptive effects of artemisinin, whereas bicuculline significantly inhibited the antinocicptive effect of artemisinin. Conclusions: It seems that antinocicptive effects of artemisinin are mediated by $GABA_A$ receptors.
Keywords
Analgesics, Opioid; Animals; Artemisinin; Gamma-Aminobutyric Acid; Inflammation; Mice; Pain; Receptors, GABA; Writhing Test;
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