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http://dx.doi.org/10.3344/kjp.2007.20.2.106

GABAB Receptor Modulation on the Antinociception of Intrathecal Sildenafil in the Rat Formalin Test  

Kim, Woong Mo (Department of Anesthesiology and Pain Medicine, Chonnam National University Medical School)
Yoon, Myung Ha (Department of Anesthesiology and Pain Medicine, Chonnam National University Medical School)
Lee, Hyung Gon (Department of Anesthesiology and Pain Medicine, Chonnam National University Medical School)
Han, Yong Gu (Department of Anesthesiology and Pain Medicine, Chonnam National University Medical School)
Kim, Yeo Ok (Department of Anesthesiology and Pain Medicine, Chonnam National University Medical School)
Huang, Lan Ji (Brain Korea 21 Project, Center for Biomedical Human Resources at Chonnam National University)
Cui, Jin Hua (Brain Korea 21 Project, Center for Biomedical Human Resources at Chonnam National University)
Publication Information
The Korean Journal of Pain / v.20, no.2, 2007 , pp. 106-110 More about this Journal
Abstract
Background: A phosphodiesterase 5 inhibitor, sildenafil, has been effective against nociception. Several lines of evidence have demonstrated the role of the GABAergic pathway in the modulation of nociception. The impact of the GABA receptors on sildenafil was studied using the formalin test at the spinal level. Methods: Male SD rats were prepared for intrathecal catheterization. The formalin test was induced by subcutaneous injection of formalin solution. The change in the activity of sildenafil was examined after pre-treatment with GABA receptor antagonists ($GABA_A$ receptor antagonist, bicuculline; $GABA_B$ receptor antagonist, saclofen). Results: Intrathecal sildenafil dose-dependently attenuated the flinching observed during phase 1 and 2 in the formalin test. The antinociceptive effect of sildenafil was reversed by the $GABA_B$ receptor antagonist (saclofen) but not by the $GABA_A$ receptor antagonist (bicuculline) in both phases. Conclusions: Intrathecal sildenafil suppressed acute pain and the facilitated pain state. The antinociception of sildenafil is mediated via the $GABA_B$ receptor, but not the $GABA_A$ receptor, at the spinal level.
Keywords
antinociception; formalin test; GABA receptors; intrathecal; rat; sildenafil;
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Times Cited By KSCI : 2  (Citation Analysis)
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