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http://dx.doi.org/10.3344/kjp.2007.20.2.100

The Effect of Treatment with Intrathecal Ginsenosides in a Rat Model of Postoperative Pain  

Shin, Dong Jin (Department of Anesthesiology and Pain Medicine, Chonnam National University Medical School)
Yoon, Myung Ha (Department of Anesthesiology and Pain Medicine, Chonnam National University Medical School)
Lee, Hyung Gon (Department of Anesthesiology and Pain Medicine, Chonnam National University Medical School)
Kim, Woong Mo (Department of Anesthesiology and Pain Medicine, Chonnam National University Medical School)
Park, Byung Yun (Department of Anesthesiology and Pain Medicine, Chonnam National University Medical School)
Kim, Yeo Ok (Department of Anesthesiology and Pain Medicine, Chonnam National University Medical School)
Huang, Lan Ji (Brain Korea 21 Project, Center for Biomedical Human Resources at Chonnam National University)
Cui, Jin Hua (Brain Korea 21 Project, Center for Biomedical Human Resources at Chonnam National University)
Publication Information
The Korean Journal of Pain / v.20, no.2, 2007 , pp. 100-105 More about this Journal
Abstract
Background: Ginseng has been used to manage various types of pain in folk medicine. This study characterized the effect of treatment with intrathecal ginsenosides, the active components of ginseng in a postoperative pain model. Methods: Male Sprague-Dawley rats were implanted with lumbar intrathecal catheters. An incision was made in the plantar surface of the hindpaw. Withdrawal thresholds following the application of a von Frey filament to the wound site were measured. To determine the role of the opioid or GABA receptors following treatment with the ginsenosides, naloxone, bicuculline (a $GABA_A$ receptor antagonist), and saclofen (a $GABA_B$ receptor antagonist) were administered intrathecally 10 min before the delivery of the ginsenosides and the changes of the withdrawal thresholds after application of the von Frey filament were Observed. Results: Treatment with the intrathecal ginsenosides increased the withdrawal threshold in a dose dependent manner. Pre-treatment with intrathecal naloxone reversed the antinociceptive effect of the ginsenosides. However, pre-treatment with intrathecal bicuculline and saclofen failed to have an effect on the activity of the ginsenosides. Conclusions: These results suggest that ginsenosides are effective to alleviate the postoperative pain evoked by paw incision. The opioid receptor, but not GABA receptors, may be involved in the antinociceptive action of the ginsenosides at the spinal level.
Keywords
analgesia; GABA receptors; ginsenosides; opioid receptor; postoperative pain; spinal cord;
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1 Nemmani KV, Ramarao P: Ginsenoside Rf potentiates U-50,488H-induced analgesia and inhibits tolerance to its analgesia in mice. Life Sci 2003; 72: 759-68   DOI   ScienceOn
2 Pertovaara A: Antinociceptivc properties of fadohnidine (MPV-2426). a novel alpha2-adrenoceptor agonist. CNS Drug Rev 2004; 10: 117-26   DOI   ScienceOn
3 Roelants F. Lavand'hommc PM: Epidural neostigmine combined with sufcntanil provides balanced and selective analgesia in early labor. Anesthesiology 2004; 101: 439-44   DOI   ScienceOn
4 Scott NB. Kehlet H: Regional anaesthcssia and surgical morbidity. Sr J Surg 1988; 75: 299-304
5 Yaksh TL, Rudy TA: Chronic catheterization of the spinal subarachnoid space. Physiol Behav 1976; 17: 1031-6   DOI   ScienceOn
6 Zahn PK, Gysbers D, Brennan TJ: Effect of systemic and intrathecal morphine in a rat model of postoperative pain. Anesthesiology 1997; 86: 1066-77   DOI   ScienceOn
7 Nah SY, Park HJ, McCleskey EW: A trace component of ginseng that inhibits $Ca^{2+}$ channels through a pertussis toxin-sensitive G protein. Proc Natl Acad Sci USA 1995; 92: 8739-43
8 Yang JC . Pang CS. Tsang SF, Ng KF: Effect of American ginseng extract (Pana s quinquefolius) on formalin-induced nociception in mice. Am J Chin Med 2001; 29: 149-54   DOI   ScienceOn
9 Yeager MP. Glass DD, Neff RK, Brinck-Johnscn T: Epidural anesthesia and analgesia in high-risk surgical patients. Anesthesiology 1987; 66: 729-36   DOI   ScienceOn
10 Grond S. Hall J, Spacek A. Hoppenbrouwers M, Richarz U. Bonnet F: Iontophoretic transderr nal system using fentanyl compared with patient-controlled intravenous analgesia using morphine for postoperative pain management. Br J Anaesth 2007; 98: 806-15   DOI   ScienceOn
11 White MJ, Berghausen EJ, Dumont SW, Tsueda K, Schroeder JA, Vogel RL, et al: Side effects during continuous epidural infusion of morphine and fentanyl. Can J Anaesth 1992; 39: 576-82   DOI
12 Mogil JS. Shin YH. McCleskey EW. Kim SC, Nah SY: Ginsenosidc Rf, a trace component of ginseng root, produces antinociccption in mice. Brain Res 1998; 792: 218-28   DOI   ScienceOn
13 Nah SY, McCleskey EW: Ginseng root extract inhibits calcium channels in rat sensory neurons through a similar path, but different receptor, as mu-type opioids. J Ethnopharmacol 1994; 42: 45-51   DOI   ScienceOn
14 Chaplan SR, Bach FW, Pogrel JW, Chung JM, Yaksh TL: Quantitative assessment of tactile allodynia in the rat paw. J Neurosci Methods 1994; 53: 55-63   DOI   ScienceOn
15 Liu CX, Xiao PG: Recent advances on ginseng research in China. J Ethncpharmacol 1992;36:27-38   DOI   ScienceOn
16 Kaku T, Miyata T, Uruno T, Sako I, Kinoshita A: Chemico-pharmacological studies on saponins of Panax ginseng C. A. Meyer. II. Pharmacological part. Arzneimittelforschung 1975; 25: 539-47
17 Choi SS. Han EJ. Han KJ. Lee HK. Suh HW: Antinociccpt ive effects of ginscnosidcs injected intraccrebrovcmricularly or intralhccally in substance P.induced pain model. Planta Med 2003; 69: 1001-4   DOI   ScienceOn
18 Brennan TJ, Vandcnnculcn EP. Gchhan GF: Characlerization of a rat model of incisional pain. Pain 1996; 64: 493-501   DOI   ScienceOn
19 Richebe P, Rivat C, Laulin JP, Maurette P, Simonnct G: Ketamine improves the management of exaggerated postoperative pain observed in perioperative fentanyl-treated rats. Anesthesiology 2005; 102: 421-8   DOI   ScienceOn
20 Yamamoto T, Sakashita Y: The role of the spinal opioid receptor like1 receptor, the NK-1 receptor, and cyclooxygenase- 2 in maintaining postoperative pain in the rat. Anesth Analg 1999; 89: 1203-8   DOI   ScienceOn
21 Nah JJ. Hahn JH. Chung S, Choi S. Kim YI, Nah SY: Effect of ginsenosides, active components of ginseng, on capsaicin-induced pain-related behavior. Neuropharmacology 2000; 39: 2180-4   DOI   ScienceOn
22 Rhim H, Kim H, Lee DY, Oh TH, Nah SY: Ginseng and ginsenoside Rg3, a newly identified active ingredient of ginseng, modulate $Ca^{2+}$ channel currents in rat sensory neurons. Eur J Pharmacol 2002; 436: 151-8   DOI   ScienceOn
23 Yoon SR. Nah JJ. Shin YH, Kim SK. Nam KY, Choi HS. et al: Ginscnosides induce differential antinociccption and inhibit substance P induced-nociceptive response in mice. Life Sci 1998; 62: PL 319-25   DOI   ScienceOn
24 Furst S: Transmitters involved in antinociception in the spinal cord. Brain Res Bull 1999; 48: 129-41   DOI   ScienceOn
25 Shin YH. Jung OM, Nah JJ. Nam KY. Kim CY. Nah SY: Glnscnosides thai produce differential antinociception in mice. Gen Phannacol 1999; 32: 653-9   DOI   ScienceOn
26 Zahn PK, Brennan TJ: Lack of effect of intrathecally administered N-methyl-D-aspartate receptor antagonists in a rat model for postoperative pain. Anesthesiology 1998; 88: 143-56   DOI   ScienceOn
27 Woolf CJ, Chong MS: Preemptive analgesia treating postoperative pain by preventing the establishment of central sensitization. Anesth Analg 1993; 77: 362-79   DOI