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Assessment for the Role of Serotonin Receptor Subtype 3 for the Analgesic Action of Morphine at the Spinal Level  

Yoon, Myung Ha (Department of Anesthesiology and Pain Medicine, Medical School, Chonnam National University)
Bae, Hong Buem (Department of Anesthesiology and Pain Medicine, Medical School, Chonnam National University)
Choi, Jeong Il (Department of Anesthesiology and Pain Medicine, Medical School, Chonnam National University)
Kim, Seok Jae (Department of Anesthesiology and Pain Medicine, Medical School, Chonnam National University)
Kim, Chang Mo (Department of Anesthesiology and Pain Medicine, Medical School, Chonnam National University)
Jeong, Sung Tae (Department of Anesthesiology and Pain Medicine, Medical School, Chonnam National University)
Kim, Kwang Su (Department of Anesthesiology and Pain Medicine, Medical School, Chonnam National University)
Jin, Won Jong (Department of Anesthesiology and Pain Medicine, Medical School, Chonnam National University)
Kim, Jong Pil (Department of Anesthesiology and Pain Medicine, Medical School, Chonnam National University)
Kim, Jong Sik (Department of Anesthesiology and Pain Medicine, Medical School, Chonnam National University)
Kim, Se Yeol (Department of Anesthesiology and Pain Medicine, Medical School, Chonnam National University)
Jeong, Chang Young (Department of Anesthesiology and Pain Medicine, Medical School, Chonnam National University)
Publication Information
The Korean Journal of Pain / v.18, no.2, 2005 , pp. 113-117 More about this Journal
Abstract
Background: Serotonin 3 receptor is involved in the modulation of nociceptive transmission in the spinal cord. The serotonin 3 receptor antagonist has been used for the management of opioid-induced nausea and vomiting. The aim of this study was to examine whether the analgesic effect of morphine is antagonized by serotonin 3 receptor antagonists at the spinal level. Methods: Rats were implanted with lumbar intrathecal catheters. For nociception, a formalin solution (5%, $50{\mu}l$) was injected into the hind paw of male Sprague-Dawley rats. To determine whether the effect of intrathecal morphine was mediated via serotonin 3 receptors, serotonin 3 receptor antagonists were intrathecally administered 10 min prior to the morphine delivery. Following the formalin injection, formalin-induced nociceptive behavior (flinching response) was observed for 60 min. Results: Intrathecal morphine produced a dose-dependent suppression of the flinches in both phases during the formalin test. The analgesic action of morphine was not reversed by serotonin 3 receptor antagonists (LY-278,584, ondansetron), which had little per se effect on the formalin-induced nociception. Conclusions: Spinal serotonin 3 receptors may not be involved in the analgesia of morphine on a nociceptive state evoked by a formalin stimulus.
Keywords
analgesia; formalin test; morphine; rat; serotonin 3 receptor; spinal cord;
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