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Evaluation of the Manual Method of Liquid-Based Uterine Cervicovaginal Cytology - By The Manual Method Based on $SurePath^{TM}$ Methodology  

Park, Jong-Myoung (Department of Cytopathology, Sung-Yoon Reference Laboratory)
Jang, Jin-Wook (Department of Cytopathology, Sung-Yoon Reference Laboratory)
Lim, So-Yeo (Department of Cytopathology, Sung-Yoon Reference Laboratory)
Suh, In-Soo (Department of Pathology, Kyungpook University School of Medicine)
Lee, Jong-Gi (Department of Cytopathology, Sung-Yoon Reference Laboratory)
Publication Information
The Korean Journal of Cytopathology / v.15, no.2, 2004 , pp. 86-91 More about this Journal
Abstract
Liquid-Based Uterine Cervicovaginal Cytology is known to be a sensitive and effective screening method for cervical neoplasm $MonoPrep^{TM},\;ThinPrep^{TM},\;and\;SurePath^{TM}$ methods have been recently used as Liquid-Based Uterine Cervicovaginal Cytology techniques, and the $SurePath^{TM}$ method has been used in Sung-Yoon Reference Laboratory since 2003. The goal of Liquid-Based Uterine Cervicovaginal Cytology is to separate cervical epithelial cells from non-target cells, red blood cells and neutrophils. This report describes a study which evaluated cellularity, stainablilty, and cellular changes of epithelial cels in samples processed using a manual technique as compared to samples processed using $SurePath^{TM}$ automated method. The samples processed by means of a manual technique contained a cellularity of epithelial cells similar to that of the samples processed using the $SurePath^{TM}$ automated method. In addition, we compared variable density gradient reagents, including dextran, dextrose, and sucrose, to $SurePath^{TM}$ gradient media in order to evaluate cell fractionation and cellularity of epithelial cells. 10% dextran of gradient media shows good fractionation. The samples processed with 10% dextran demonstrated sufficient cellularity of epithelial cells and shows the fewest cellular changes. In conclusion, using a manual technique on these samples is easier to read than those results obtained using the $SurePath^{TM}$ automated method.
Keywords
Liquid-Based cytology; Uterine cerrix; $SurePath^{TM}$ method; Manual technique; Gradient media;
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