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Animal Skin Pigmentation Model Using Full Thickness Skin Graft in C57BL/6 Mouse  

Lee, Hong-Ki (Image Plastic Surgery Clinic)
Park, Jong-Lim (Banobagi Plastic and Aesthetic Clinic)
Heo, Eun-Ju (Department of Plastic and Reconstructive Surgery, Seoul National University College of Medicine)
Kim, Suk-Wha (Department of Plastic and Reconstructive Surgery, Seoul National University College of Medicine)
Publication Information
Archives of Plastic Surgery / v.38, no.6, 2011 , pp. 725-732 More about this Journal
Abstract
Purpose: Skin grafting is one of the most commonly used methods in reconstructive plastic surgery field, but complications such as color change, contracture or hypertrophy are common problems. However, pathophysiology of the color change after skin graft is not yet determined and no animal model is established. Methods: Full thickness skin grafts were performed on the dorsum of C57BL/6 mice. Serial chronological gross inspection for color change and pigmentation were examined. Melanin pigments were traced by Fontana-Masson staining and semi-quantitative analysis was performed. In addition, immunohistochemical staining of S-100, Micropthalmia related Transcription Factor (MITF) and Melan-A antibodies were also performed to observe melanocytes and their changes. Results: After skin graft, color change and pigment spots were observed in the graft. Fontana-Masson staining showed melanin pigments in the epidermal and dermal layers in all mice. Immunohistochemistry staining to S-100, MITF, Melan-A antibodies showed melanocytes at the basal layer of epidermis and dermis. Conclusion: In conclusion, we have established an animal model for skin pigmentation after skin graft. We believe this study may be useful in understanding of the behavior of melanocytes after skin graft.
Keywords
C57BL/6 mouse; Skin graft; Pigmentation; Melanocyte; Premature melanocyte;
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