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The Anti-Inflammatory Effect of Pegmatite by in Vivo and in Vitro Study  

Lee, Min-Hyuk (Department of Plastic & Reconstructive Surgery, College of Medicine, Dong-A University)
Kim, Seok-Kwun (Department of Plastic & Reconstructive Surgery, College of Medicine, Dong-A University)
Kwon, Yong-Seok (Department of Plastic & Reconstructive Surgery, College of Medicine, Dong-A University)
Lee, Jang-Ho (Department of Plastic & Reconstructive Surgery, College of Medicine, Dong-A University)
Lee, Keun-Cheol (Department of Plastic & Reconstructive Surgery, College of Medicine, Dong-A University)
Publication Information
Archives of Plastic Surgery / v.37, no.1, 2010 , pp. 12-21 More about this Journal
Abstract
Purpose: This work aimed to elucidate the anti-inflammatory effect of pegmatite in vitro and in vivo. Methods: Author evaluated the suppressive effects of pegmatite on lipopolysaccharide (LPS)-stimulated nitric oxide (NO) production, TNF-${\alpha}$ and IL-6 release in the RAW 264.7 murinemacrophages. Results: Treatment of RAW 264.7 cells with pegmatite significantly reduced LPS-stimulated NO production and inflammatory cytokine such as TNF-${\alpha}$ and IL-6 secretion in a concentration-dependent manner. Also pegmatite showed topical anti-inflammatory activity in the arachidonic acid (AA)-induced ear edema and acetic acid-induced increase in capillary permeability assessment in mice. It was also found that pegmatite (10 mg per ear in DW) inhibited arachidonic acid induced edema at 24 h more profoundly than 1 h by topical application. Furthermore, the vascular permeability increase induced by acetic acid was significantly reduced in mice that received pegmatite in 50 mg per mouse. Conclusion: Therefore the results of the present study suggest that pegmatite is a potent inhibitor of the LPS-induced NO and inflammatory cytokine in RAW 264.7 macrophages and showed anti-inflammatory activities in vivo animal model.
Keywords
Pegmatite; Anti-inflammatory activity; Ear edema; Vascular permeability; NO; LOX;
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