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The Effect of Various Methods of Cross-linking in Type I Collagen Scaffold on Cartilage Regeneration  

Son, Dae Gu (Department of Plastic and Reconstructive Surgery, Keimyung University, Dongsan Medical Center)
Lim, Joong Jae (Duo Plastic Surgery Clinic)
Sohn, Kyounghee (Department of Plastic and Reconstructive Surgery, Keimyung University, Dongsan Medical Center)
Yang, Eun Kyung (Bioland)
Publication Information
Archives of Plastic Surgery / v.33, no.6, 2006 , pp. 723-731 More about this Journal
Abstract
Purpose: Collagen is the principal structural biomolecule in cartilage extracellular matrix, which makes it a logical target for cartilage engineering. In this study, porous type I collagen scaffolds were cross-linked using dehydrothermal(DHT) treatment and/or 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide(EDC), in the presence and absence of chondroitin-6-sulfate(CS) for cartilage regeneration. Methods: Cartilage defects were created in the proximal part of the ear of New Zealand rabbits. Four types of scaffolds(n=4) were inserted. The types included DHT cross-linked(Group 1), DHT and EDC cross- linked(Group 2), CS added DHT cross-linked(Group 3), and CS added DHT and EDC cross-linked(Group 4). Histomorphometric analysis and cartilage-specific gene expression of the reconstructed tissues were evaluated respectively 4, 8, and 12 weeks after implantation. Results: The largest quantity of regenerated cartilage was found in DHT cross-linked groups 1 and 3 in the 8th week and then decreased in the 12th week, while calcification increased. Calcification was observed from the 8th week and the area increased in the 12th week. Group 4 was treated with EDC cross-linking and CS, and the matrix did not degrade in the 12th week. Cartilage-specific type II collagen mRNA expression increased with time in all groups. Conclusion: CS did not increase chondrogenesis in all groups. EDC cross-linking may prevent chondrocyte infiltration from the perichondrium into the collagen scaffold.
Keywords
Collagen type I; Ear cartilages; Chondroitin sulfates;
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1 JS Kang: Plastic surgery. 3rd ed, Seoul, Koonja, 2004, p 290
2 Rhie JW, Ahn TJ, Park JG, Sohn JY, Cho HS, Lim P: Culture of chondrocytes on scaffolds with different pore network of PLGA and PLLA. J Korean Soc Plast Reconstr Surg 30: 237, 2003
3 van Susante JL, Pieper J, Burna P, van Kuppevelt TH, van Beuningen H, van Der Kraan PM, Veerkamp JH, van den Berg WB, Veth RPH: Linkage of chondroitin-Sulfate to type I collagen scaffolds stimulates the bioactivity of seeded chondrocytes in vitro. Biomaterials 22: 2359, 2001   DOI   ScienceOn
4 Jeong JL, Shim HG, Park JG, Ju YM, Han DK, Rhie JW, Ahn ST: The effect of plasma treatment of Poly(lactic-coglycolic)( PLGA) scaffold on adhesion and bioactivity of cultured chondrocytes. J Korean Soc Plast Reconstr Surg 30: 491, 2003
5 Yang EK, Park JK, Ahn J II, Lee HG, Seo SJ, Son DC, Kim JC, Song KY: Tissue-engineered skin substitutes using collagen scaffold with amniotic membrane component. Key Engineering Materials 12: 277, 2005
6 Ruuskanen MM, Virtanen MK, Tuominen H, T6rrnalii P, Waris T: Generation of cartilage from auricular and rib free perichondrial grafts around a self-reinforced polyglycolic acid mould in rabbits. Scand J Plast Reconstr Surg Hand Surg 28: 81, 1994   DOI   ScienceOn
7 Grant WT, Wang GJ, Balian G: Type X collagen synthesis during endochondral ossification in fracture repair. J Biol Chem 262: 9844, 1987
8 Kim WS, Vacanti JP, Cima L, Mooney D, Upton J, Puelacher WC, Vacanti CA: Cartilage engineered in predetermined shapes employing cell transplantation on synthetic biodegradable polymers. Plast Reconstr Surg 94: 233, 1994   DOI   ScienceOn
9 Bean JK, Verwoerd-Verhoef HL, Verwoerd m: Reconstruction of the anterior laryngeal wall with a composite graft of demineralized bovine bone matrix and autogenous perichondrium; an experimental study in adult rabbits. ORL J Otorhinolaryngol Relat Spec 56: 224, 1994   DOI   ScienceOn
10 Lee HG, Son DC, Han KH, Kim JH, Lee SY: Chondrogenic effect of transplanted type I collagen scaffold within subperichondrial cartilage defect. J Korean Soc Plast Reconstr Surg 32: 521, 2005
11 Pachence JM: Collagen-based devices for soft tissue repair. J Biomed Mater Res 33: 35, 1996   DOI
12 Sohn SA, Ohlsen L: Growth of cartilage from a free perichondrial graft placed across a defect in a rabbit's trachea. Plast Reconstr Surg 53: 55, 1974   DOI   ScienceOn
13 Randolph MA, Anseth K, Yaremchuk MJ: Tissue engineering of cartilage. Clin piast Surg 30: 519, 2003   DOI   ScienceOn
14 Kim JS, Son DC, Han KH, Choi DW, Park KK: Histologic study of coral template wrapped with perichondrial flap. J Korean Soc Plast Reconstr Surg 26: 392, 1999
15 Pieper JS, Oosterhof A, Dijkstra PI, Veerkamp JH, van Kuppevelt TH: Preparation and characterization of porous crosslinked collagenous matrices containing bioavailable chondroitin sulphate. Biomaterials 20: 847, 1999
16 Shen G: The role of type X collagen in facilitating and regulating endochondral ossification of articular cartilage. Orthod Craniofac Res 8: 11, 2005   DOI   ScienceOn