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The Effect of the Transcriptional Regulation of Sp1 for TGF-β1 and CTGF Expression in Scar Formation  

Park, Dong Man (Department of Plastic and Reconstructive Surgery, Keimyung University School of Medicine)
Sohn, Dae Gu (Department of Plastic and Reconstructive Surgery, Keimyung University School of Medicine)
Han, Ki Hwan (Department of Plastic and Reconstructive Surgery, Keimyung University School of Medicine)
Lee, Sun Young (Department of Medical Research Institute, Kyungpook National University)
Chae, Young Mi (Department of Pathology, College of Medicine, Catholic University of Daegu)
Chang, Young Chae (Department of Pathology, College of Medicine, Catholic University of Daegu)
Park, Kwan Kyu (Department of Pathology, College of Medicine, Catholic University of Daegu)
Publication Information
Archives of Plastic Surgery / v.33, no.1, 2006 , pp. 39-45 More about this Journal
Abstract
This study is to examine the relationship between TGF-b1 expression and CTGF expression, and to evaluate the effect of Sp1 blockade on the expression of TGF-b1, CTGF and extracellular genes, clones of fibroblasts stably transfected with Sp1 decoy ODN. R-Sp1 decoy ODN was highly resistant to degradation by nucleases or serum, compared to the linear or phosphorothioated-Sp1 decoy ODN. Skin wounds were created on the back of 36 anesthetized rats. They were divided into four groups-the rats with normal skin, with wounded skin without decoy, with wounded skin injected with R-Sp1 decoy, and with wounded skin injected with mismatched R-Sp1 decoy, respectively. Skins were collected at 3rd, 5th, 7th, 14th day after wounding. Cellular RNA was extracted by RT-PCR analysis. TGF-${\beta}1$ and CTGF were deeply related with skin fibrosis during scar formation and it appeared that TGF-${\beta}1$ may cause the induction of CTGF expression. R-Sp1 decoy ODN inhibited TGF-${\beta}1$ and CTGF expression both in cultured fibroblasts and in the skin of rats. These results indicate that targeting Sp1 with R-type decoy efficiently blocks extracellular matrix gene expression, and suggest an important new therapeutic approach to control the scarring in normal wound healing and fibrotic disorders.
Keywords
Scar Formation; Sp1 decoy oligodeoxynucleotide (ODN); Transforming growth factor(TGF)-${\beta}1$; Connective tissue growth factor(CTGF);
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