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http://dx.doi.org/10.1007/s43188-020-00084-9

Flavonoid fractions of diosmin and hesperidin mitigate lead acetate-induced biochemical, oxidative stress, and histopathological alterations in Wistar rats  

Lamidi, Ibrahim Yusuf (Department of Veterinary Pharmacology and Toxicology, University of Maiduguri)
Mikail, Hudu Garba (Department of Veterinary Pharmacology and Toxicology, University of Abuja)
Adamu, Sani (Department of Veterinary Pathology, Ahmadu Bello University Zaria)
Akefe, Isaac Oluwatobi (Department of Physiology, Biochemistry, and Pharmacology, Faculty of Veterinary Medicine, University of Jos)
Tijjani, Mohammed Bashir (Department of Veterinary Pharmacology and Toxicology, University of Maiduguri)
Salihu, Sabo Isa (Department of Veterinary Pharmacology and Toxicology, University of Maiduguri)
Olatunji, Aisha Omobolanle (Department of Veterinary Pharmacology and Toxicology, University of Ilorin)
Hassan, Abdussalam (Department of Veterinary Pathology, University of Maiduguri)
Daniel, Nubwa (Department of Veterinary Pharmacology and Toxicology, University of Maiduguri)
Adegoke, Victoria Aderonke (Department of Science and Laboratory Technology, Ekiti State University)
Publication Information
Toxicological Research / v.37, no.4, 2021 , pp. 473-484 More about this Journal
Abstract
This study aims at investigating the protective effects of flavonoid fractions of diosmin and hesperidin in mitigating sub-chronic lead acetate-induced biochemical, oxidative stress, and histopathological alterations in adult male Wistar rats. Forty animals were randomly assigned into five groups, each consisting of eight animals. Group I animals was treated with deionised water only, group II, IV, and V were administered lead acetate 90 mg/Kg body weight (1/20th of the LD50), groups III, and IV was administered Daflon (100 mg/Kg), while group V was administered Daflon (200 mg/Kg), 30 min prior treatment with lead acetate. All treatments lasted for 42 days. Blood lead levels, electrolyte parameters, zinc protoporphyrin (ZPP) levels, activities of antioxidant enzymes, and histopathology of vital organs, were evaluated following standard practice. Sub-chronic lead acetate exposure induced a decrease in levels of serum electrolytes, and activities of antioxidant enzymes, while blood lead levels, ZPP, and malondialdehyde levels were increased. Lead exposure also instigated marked variation in histopathology of vital organs. Conversely, co-treatment with graded doses of daflon improved the levels of blood lead, electrolytes, ZPP, activities of antioxidant enzymes, and histopathology of vital organs. Data obtained from the current study indicate that rats exposed to sub-chronic doses of lead acetate show increased blood lead levels, electrolyte imbalance, alongside impairment in ZPP levels, activities of antioxidant enzymes, and histopathology, while pretreatment using daflon mitigated the ensued perturbations. This, therefore, suggests that consumption of foods enriched with flavonoid fractions of diosmin and hesperidin may be beneficial for individuals inhabiting lead-polluted environments.
Keywords
Hesperidin; Flavonoids; Diosmin; Biochemical; Zinc protoporphyrin; Oxidative stress;
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1 Shalana M, Mostafab M, Hassounab M (2005) Amelioration of lead toxicity on rat liver with vitamin and silymarin supplements. Toxicology 206:1-15. https://doi.org/10.1016/j.tox.2004.07.006   DOI
2 Lawton LJ, Donaldson WE (1991) Lead-induced tissue fatty acid alterations and lipid peroxidation. Biol Trace Elem Res 28:83-97. https://doi.org/10.1007/BF02863075   DOI
3 Johar D, Roth JC, Bay GH, Walker JN, Kroczak TJ, Los M (2004) Inflammatory response, reactive oxygen species, programmed (necrotic-like and apoptotic) cell death and cancer. Roczniki Akademia Medyczna Bialymstoku 49:31-39
4 Burki T (2020) Report says 815 million children have high blood lead levels. Lancet 396:370. https://doi.org/10.1016/S0140-6736(20)31684-6   DOI
5 Hedayati A, Darabitabar F (2017) Lethal and sub-lethal impacts of lead on some hematological, biochemical and immunological indices in pian roach. Pollution 3:21-27. https://doi.org/10.22059/poll.2017.59567   DOI
6 Ameh MP, Mohammed M, Ofemile YP, Mohammed MG, Gabriel A, Isaac AO (2020) Detoxifying Action of Aqueous Extracts of Mucuna pruriens Seed and Mimosa pudica Root Against Venoms of Naja nigricollis and Bitis arietans. Recent Pat Biotechnol 14:134-144. https://doi.org/10.2174/1872208313666191025110019   DOI
7 Yabe J, Nakayama SM, Nakata H, Toyomaki H, Yohannes YB, Muzandu K, Kataba A, Zyambo G, Hiwatari M, Narita D, Yamada D, Hangoma P, Munyinda NS, Mufune T, Ikenaka Y, Choongo K, Ishizuka M (2020) Current trends of blood lead levels, distribution patterns and exposure variations among household members in Kabwe, Zambia. Chemosphere 243:125412. https://doi.org/10.1016/j.chemosphere.2019.125412   DOI
8 Wani AL, Ara A, Usmani JA (2015) Lead toxicity: a review. Interdiscip Toxicol 8:55-64. https://doi.org/10.1515/intox-2015-0009   DOI
9 Gems D, Patridge L (2008) Stress-response hormesis and aging: that which does not kill us makes us stronger. Cell Metab 7:200-203. https://doi.org/10.1016/j.cmet.2008.01.001   DOI
10 Wei W, Wu X, Bai Y, Li G, Feng Y, Meng H, Li H, Li M, Zhang X, He M, Guo H (2020) Lead exposure and its interactions with oxidative stress polymorphisms on lung function impairment: results from a longitudinal population-based study. Environ Res 187:109645. https://doi.org/10.1016/j.envres.2020.109645   DOI
11 Shubina VS, Shatalin YV (2017) Antioxidant and iron-chelating properties of taxifolin and its condensation product with glyoxylic acid. J Food Sci Technol 54:1467-1475. https://doi.org/10.1007/s13197-017-2573-0   DOI
12 Rendon-Ramirez A, Cerbon-Solorzano J, Maldonado-Vega M, Quintanar-Escorza MA, Calderon-Salinas JV (2007) Vitamin E reduces the oxidative damage on d-aminolevulinic dehydratase induced by lead intoxication in rat erythrocytes. Toxicol In Vitro 21:1121-1126. https://doi.org/10.1016/j.tiv.2007.04.019   DOI
13 Ayla O, Metin O (2015) Biochemistry of reactive oxygen and nitrogen species. Faculty of Veterinary Medicine, University of Kafkas, Turkey, Croatia, InTech, pp 37-58. https://doi.org/10.5772/61193   DOI
14 Adinortey MB, Sarfo JK, Kwarteng J, Adinortey CA, Ekloh W, Kuatsienu LE, Kwadwo Nyarko A (2018) The Ethnopharmacological and Nutraceutical Relevance of Launaea taraxacifolia (Willd.) Amin ex C. Jeffrey. Evid Based Complement Alternat Med 2018:7259146. https://doi.org/10.1155/2018/7259146   DOI
15 Agency for Toxic Substances and Disease Registry (ATSDR) (2020) Toxicological profile for Lead. U.S. Department of Health and Human Services, Public Health Service, Atlanta, GA. https://doi.org/10.15620/cdc:95222   DOI
16 Bokara KK, Brown E, McCormick R, Yallapragada PR, Rajanna S, Bettaiya R (2008) Lead-induced increase in antioxidant enzyme and lipid peroxidation products in developing rat brain. Biometals 21:9-16. https://doi.org/10.1007/s10534-007-9088-5   DOI
17 Isaac A, Ibrahim Y, Andrew A, Edward D, Solomon A (2017) The cortisol steroid levels as a determinant of health status in animals. J Proteomics Bioinform 10:277-283. https://doi.org/10.4172/jpb.1000452   DOI
18 Akefe IO, Ayo JO, Sinkalu VO (2020) Kaempferol and zinc gluconate mitigate neurobehavioral deficits and oxidative stress induced by noise exposure in Wistar rats. PLoS ONE 15:e0236251. https://doi.org/10.1371/journal.pone.0236251   DOI
19 Lamidi IY, Hudu MG, Akefe IO, Adamu S, Salihu SI (2020) Sub-chronic administration of flavonoid fraction Daflon improve lead-induced alterations in delta-aminolevulinic acid dehydratase activity, erythrocytic parameters, and erythrocyte osmotic fragility in Wistar rats. Comp Clin Pathol. https://doi.org/10.1007/s00580-020-03144-6   DOI
20 Lamidi IY, Akefe IO (2017) Mitigate effects of antioxidants in lead toxicity. Clin Pharmacol Toxicol J 1:1-9
21 Ramlet AA (2001) Clinical benefits of Daflon 500 mg in the most severe stages of chronic venous insufficiency. Angiology 52:49-56. https://doi.org/10.1177/000331970105200107   DOI
22 Erick H, Yong W, Ian DB (2016) A novel methodology for rapid digestion of rare earth element ores and determination by microwave plasma-atomic emission spectrometry and dynamic reaction cell-inductively coupled plasma-mass spectrometry. Talanta 160:521-527. https://doi.org/10.1016/j.talanta.2016.07.067   DOI
23 Grandjean P (1979) Occupational lead exposure in Denmark: screening with a haematofluorimeter. Br J Ind Med 36:52-58. https://doi.org/10.1136/oem.36.1.52   DOI
24 Garber JC, Barbee RW, Bielitzki JT, Clayton LA, Donovan JC, Kohn DF, Lipman NS et al (2011) Guide for the care and use of laboratory animals, 8th edn. National Academies Press, Washington, DC
25 Prabhu VV, Sathyamurthy D, Ramasamy A, Das S, Anuradha M, Pachiappan S (2016) Evaluation of protective effects of diosmin (a citrus flavonoid) in chemical-induced urolithiasis in experimental rats. Pharm Biol 54:1513-1521. https://doi.org/10.3109/13880209.2015.1107105   DOI
26 Palgia DE, Valentine WN (1967) Studies on qualitative and quantitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med 70:158-169
27 Beers FN, Sizer IW (1952) A spectrophotometric method for measuring the break-down of hydrogen peroxide by catalase. J Biol Chem 195:133-140   DOI
28 Brodowska MK (2017) Natural flavonoids: classification, potential role, and application of flavonoid analogues. Eur J Biol Res 7:108-123. https://doi.org/10.5281/zenodo.545778   DOI
29 Rizk SM, Sabri NA (2009) Evaluation of clinical activity and safety of Daflon 500 mg in type 2 diabetic female patients. Saudi Pharm J 17:199-207. https://doi.org/10.1016/j.jsps.2009.08.008   DOI
30 Patra RCD, Swarup SK, Dwivedi AS (2001) Trace minerals in blood of young calves during exposure to lead. Indian J Animal Sci 71:507-510. https://doi.org/10.4061/2011/457327   DOI
31 Sakai T (2000) Biomarkers of lead exposure. Ind Health 38:127-142. https://doi.org/10.2486/indhealth.38.127   DOI
32 Gajawat S, Sancheti G, Goyal P (2005) Vitamin C against concomitant exposure to heavy metal and radiation: a study on variations in hepatic cellular counts. Asian J Exposure Sci 19:53-58. https://doi.org/10.1007/s12291-013-0375-3   DOI
33 Patra RC, Rautray AK, Swarup D (2011) Oxidative stress in lead and cadmium toxicity and its amelioration. Vet Med Int 22:1-9. https://doi.org/10.4061/2011/457327   DOI
34 Kayode IMO, Olugbenga IE (2017) Lead acetate induced cerebral tissue damage; The effect of Phoenix dactylifera pits extract. Eur J Med Plants 21:1-9. https://doi.org/10.9734/EJMP/2017/37302   DOI
35 Julka D, Pal R, Gill KD (1992) Neurotoxicity of dichlorvos: effect on antioxidant system in the rat central nervous system. Exp Mol Pathol 56:144-152. https://doi.org/10.1016/0014-4800(92)90031-6   DOI
36 Yasim A, Ozbag D, Kilinc M, Ciralik H, Toru I (2011) The effects of diosmin-hesperidin combination treatment on the lipid profile and oxidative antioxidative systems in high-cholesterol diet-fed rats. Turkey Gorgus Kalp Damar Cerrahisi Dergisi 1:55-61. https://doi.org/10.5897/IJMMS.9000121   DOI
37 Akefe IO, Yusuf IL, Adegoke VA (2019) C-glycosyl flavonoid orientin alleviates learning and memory impairment by radiofrequency electromagnetic radiation in mice via improving antioxidant defence mechanism. Asian Pac J Trop Biomed 9:518-523. https://doi.org/10.4103/2221-1691.271725   DOI
38 Draper HH, Hadley M (1990) Malondialdehyde determination as index of lipid peroxidation. Methods Enzymol 186:421-431. https://doi.org/10.1016/0076-6879(90)86135-i   DOI
39 Kobo PI, Ayo JO, Aluwong T, Zezi AU, Maikai V, Ambali SF (2014) Flavonoid mixture ameliorates increase in erythrocyte osmotic fragility and malondialdehyde concentration induced by Trypanosoma brucei brucei-infection in Wistar rats. Res Vet Sci 96:139-142. https://doi.org/10.1016/j.rvsc.2013.10.005   DOI
40 Martin JP Jr, Dailey M, Sugarman E (1987) Negative and positive assays of superoxide dismutase based on haematoxylin autoxidation. Arch Biochem Biophys 225:329-336. https://doi.org/10.1016/0003-9861(87)90400-0   DOI
41 Nisar NA, Sultana M, Waiz HA, Para PA, Baba NA, Zargar FA, Raja WH (2013) Experimental study on the effect of vitamin C administration on lipid peroxidation and antioxidant enzyme activity in rats exposed to chlorpyriphos and lead acetate. Vet World 6:461-466. https://doi.org/10.5455/vetworld.2013.461-466   DOI
42 Jalali MS, Seyedeh NH, Mousavi M (2017) Comparative effect of silymarin and D-penicillamine on lead induced hemotoxicity and oxidative stress in rat. Iranian J Toxicol 11:12-18. https://doi.org/10.29252/arakmu.11.3.11   DOI
43 Eman G, Fawzi B (2020) Micronized flavonoid fraction Daflon 500 protects heart against ischemia-reperfusion injury: an old medicine for a new target. All Life 13:556-568. https://doi.org/10.1080/26895293.2020.1832921   DOI
44 Eze JI, Anene BM, Chukwu CC (2008) Determination of serum and organ malondialdehyde (MDA) concentration, a lipid peroxidation index in Trypanosoma bruceiinfected rats. Comp Clin Pathol 17:67-72. https://doi.org/10.1007/s00580-008-0722-6   DOI
45 Isaac AO, Joseph AO, Victor SO, Lamidi YI, Andrew AM (2017) Ameliorative effects of kaempferol and zinc gluconate on erythrocyte osmotic fragility and haematological parameters in Wistar rats exposed to noise stress. Insights Biomed 2:15. https://doi.org/10.21767/2572-5610.100031   DOI
46 Ozkaya A, Sahin Z, Kuzu M, Selim Y, Mustafa S, Mirac O, Ertan U, Veysel Y, Ramazan C, Yologlu S (2017) Role of geraniol against lead acetate-mediated hepatic damage and their interaction with liver carboxylesterase activity in rats. Arch Physiol Biochem 17:1-8. https://doi.org/10.1080/13813455.2017.1364772   DOI
47 Wang J, Zhu H, Yang Z, Liu Z (2013) Antioxidative effects of hesperetin against lead acetate-induced oxidative stress in rats. Indian J Toxicol 45:395-398. https://doi.org/10.4103/0253-7613.115015   DOI
48 Sidhu P, Nehru B (2004) Lead intoxication: histological and oxidative damage in rat cerebrum and cerebellum. J Trace Elements Exp Med 17:45-53. https://doi.org/10.1002/jtra.10052   DOI
49 Luna GH (1960) Manual of histologic staining method of armed forces institute of pathology, 35th edn. McGraw-Hill Book Company, New York, p 46
50 Suradkar SG, Ghodasara DJ, Vihol P, Patel J, Jaiswal V, Prajapati KS (2009) Haematobiochemical alterations induced by lead acetate toxicity in wistar rats. Vet World 2:429-439
51 Aksu DS, Didin M, Kayikci F (2012) The protective role of polyphenols on blood cells in rats exposed to lead. Rev Romana Med Lab 20:233-243
52 Okediran BS, Biobaku KT, Olaifa FH, Atata AJ (2017) Haematological and antioxidant enzyme response to lead toxicity in male Wistar rats. Ceylon J Sci 46:31-37. https://doi.org/10.4038/cjs.v46i2.7427   DOI
53 Flora SJ, Flora G, Saxena G, Mishra M (2007) Arsenic and lead induced free radical generation and their reversibility following chelation. Cell Mol Biol 53:26-47. https://doi.org/10.1170/T773   DOI
54 Bah H, Bandeira MJ, Gomes-Junior EA, Anjos A, Rodrigues Y, Dos Santos NR, Martinez VO, Rocha R, Costa RG, Adorno EV, Menezes-Filho JA (2020) Environmental exposure to lead and hematological parameters in Afro-Brazilian children living near artisanal glazed pottery workshops. J Environ Sci Health 55:964-974. https://doi.org/10.1080/10934529.2020.1761738   DOI
55 Ujowundu CO, Okwu GN, Achilike JJ, Nwaogu LA, Iheme CI (2017) Lead-induced oxidative stress and chemoprotective role of dietary supplements on Wistar albino rats. Annu Res Rev Biol 13:1-14. https://doi.org/10.9734/ARRB/2017/33167   DOI
56 Gurer H, Ozgunes H, Neal R, Spitzand DR, Ercal N (1998) Antioxidant effects of N-acetyl cysteine and succimer in red blood cells from lead-exposed rats. Toxicology 128:181-189. https://doi.org/10.1016/j.tox.2004.07.006   DOI
57 Altuntas I, Delibas N, Sutcu R (2002) The effects of organophosphate insecticide methidathion on lipid peroxidation and anti-oxidant enzymes in rat erythrocytes: role of vitamins E and C. Hum Exp Toxicol 21:681-685. https://doi.org/10.1191/0960327102ht304oa   DOI