Browse > Article
http://dx.doi.org/10.1007/s43188-020-00074-x

Teratogenic effects of ethanol extract of Curcuma mangga Val. rhizomes in wistar rats  

Yuandani, Yuandani (Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara)
Tarigan, Krisna Sandra Amalia (Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara)
Yuliasmi, Sri (Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Sumatera Utara)
Publication Information
Toxicological Research / v.37, no.4, 2021 , pp. 429-434 More about this Journal
Abstract
We have recently highlighted the immunomodulatory effect of ethanol extract of Curcuma mangga Val. rhizomes. The current study was performed to investigate the teratogenic effects of C. mangga extract in Wistar rats. The C. mangga extract at doses of 100, 500 and 1000 mg/kg bw were administered to pregnant rats on day 6-15 of gestation. The litter size, length and birth weight as well as body weight of pregnant rats were determined to evaluate the teratogenic effects of C. mangga extract. External and skeletal malformations were also examined. The extract reduced the litter length compared to normal control (p<0.05). The average body weight gain of the pregnant rats also decreased. Resorption was observed after treatment with extract at the dose of 1000 mg/kg bw. The extract at the doses of 500 and 1000 mg/kg bw reduced litter birth weight and induced external and skeletal malformations. This demonstrates that ethanol extract of C. mangga has teratogenic effects in Wistar rats and should be used with caution in pregnancy.
Keywords
C. mangga; Teratogenic effect; Pregnancy; Malformations;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Ruangsang P, Tewtrakul S, Reanmongkol W (2010) Evaluation of the analgesic and anti-inflammatory activities of Curcuma mangga Val and Zijp rhizomes. J Nat Med 64:36. https://doi.org/10.1007/s11418-009-0365-1   DOI
2 Yuandani, Yuliasmi S (2018) Curcuminoid analysis in Curcuma mangga rhizomes. Asian J Pharm Clin Res 11:129-131. https://doi.org/10.22159/ajpcr.2018.v11s1.26586   DOI
3 Uche-Nwachi EO, McEwen C (2010) Teratogenic effect of the water extract of bitter gourd (Momordica charantia) on the sprague dawley rats. Afr J Tradit Complement Altern Med 7:24-33. https://doi.org/10.4314/ajtcam.v7i1.57228   DOI
4 Van der Nat JM, Klerx JPAM, De Silva KTD, Labadie RP (1987) Immunomodulatory activity of an aqueous extract of Azadirachta indica stem bark. J Ethnopharmacol 19:125-213. https://doi.org/10.1016/0378-8741(87)90036-5   DOI
5 Hande MP, Veena K (1993) Teratogenic effect of hyperthermia during early organogenesis period in mice. Teratog Carcinog Mutagen 13:145-150. https://doi.org/10.1002/tcm.1770130305   DOI
6 Shu Y, Cao M, Yin ZQ, Li P, Li TQ, Long XF, Zhu LF, Jia RY, Dai SJ, Zhao J (2015) The reproductive toxicity of saponins isolated from Cortex Albiziae in female mice. Chin J Nat Med 13:119-126. https://doi.org/10.1016/S1875-5364(15)60015-2   DOI
7 Alafiatayo AA, Lai KS, Syahida A, Mahmood M, Shaharuddin NA (2019) Phytochemical evaluation, embryotoxicity, and teratogenic effects of Curcuma longa extract on zebrafish (Danio rerio). Evid Based Complement Altern Med. https://doi.org/10.1155/2019/3807207   DOI
8 Wilson JG, Warkany J (1993) Teratology-principles and techniques. University of Chicago Press, London. https://doi.org/10.1002/jps.2600540944   DOI
9 Rugh R (1968) The mouse: its reproduction and development. Burger Publishing Co, New York
10 Levi MM, Manahan J, Mandl I (1969) Specific resorption of the mouse fetus. Obstet Gynecol 33:11-19. https://doi.org/10.1097/00006250-196901000-00002   DOI
11 Olayaki LA, Olatunji-Bello I, Soladoye AO, Jimoh OR, Ghazal O, Ighodalo M (2009) Effects of aqueous leaf extract of Cajanus cajan on litter size and serum progesterone in pregnant rats. J Pharmacognosy Phytother 1:021-024. https://doi.org/10.5897/JPP.9000021   DOI
12 Posfai E, Banhidy F, Czeizel AE (2014) Teratogenic effect of hydroxyethylrutoside, a flavonoid derivate drug-a population-based e-control study. J Matern Fetal Neonatal Med 27:1093-1098. https://doi.org/10.3109/14767058.2013.850485   DOI
13 Wu JY, Lin CY, Lin TW, Ken CF (2007) Curcumin affects development of zebrafish embryo. Biol Pharm Bull 30:1336-13369. https://doi.org/10.1248/bpb.30.1336   DOI
14 Kim D, Kim S, Kang S, Jin E (2009) Curcumin inhibits cellular condensation and alters microfilamen organization during chondogenic differentiation of limb bud mesenchymal cells. Exp Mol Med 41:656-664. https://doi.org/10.3858/emm.2009.41.9.072   DOI
15 Yuandani, Yuliasmi S, Satria DF, Dongoran RS, Sinaga MHA, Marpaung N (2019) Correlation between the phytochemical constituents of Curcuma mangga and its immunomodulatory effect. Rasayan J Chem 12:1-6. https://doi.org/10.31788/RJC.2019.1215050   DOI
16 Cox PA (1994) The ethnobotanical approach to drug discovery: strengths and limitations. Ciba Found Symp 185:25-36. https://doi.org/10.1002/9780470514634   DOI
17 McQueen C (2010) Comprehensive toxicology. Elsevier Science, Burlington
18 Vargesson N (2015) Thalidomide-induced teratogenesis: history and mechanisms. Birth Defects Res C Embryo Today 105:140-156. https://doi.org/10.1002/bdrc.21096   DOI
19 Hyoun SC, Obican SG, Scialli AR (2012) Teratogen update: methotrexate. Birth Defects Res A Clin Mol Teratol 94:187-207. https://doi.org/10.1002/bdra.23003   DOI
20 Fath B (2008) Encyclopedia of ecology. Elsevier Science, Burlington
21 Chahoud I, Paumgartten FJR (2009) Influence of litter size on the postnatal growth of rat pups: is there a rationale for litter-size standardization in toxicity studies? Environ Res 109:1021-1027. https://doi.org/10.1016/j.envres.2009.07.015   DOI
22 Malek SNA, Lee GS, Hong SL, Yaacob H, Wahab NA, Weber JFF (2011) Phytochemical and cytotoxic investigations of Curcuma mangga rhizomes. Molecules 16:4539-4548. https://doi.org/10.3390/molecules16064539   DOI
23 Tuchman D (1975) Drug effect on the fetus. Adis Press, New York
24 Karsono AH, Tandrasasmita OM, Tjandrawinata RR (2014) Molecular effects of bioactive fraction of Curcuma mangga (DLBS4847) as a downregulator of 5α-reductase activity pathways in prostatic epithelial cells. Cancer Manag Res 6:267-278. https://doi.org/10.2147/CMAR.S61111   DOI