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http://dx.doi.org/10.5487/TR.2019.35.4.371

Toxicological Evaluation of Saposhnikoviae Radix Water Extract and its Antihyperuricemic Potential  

Kim, Chang Won (Department of Oriental Medicine and Biotechnology, Kyung Hee University)
Sung, Jae Hyuck (Korea Conformity Laboratories)
Kwon, Jeong Eun (Department of Oriental Medicine and Biotechnology, Kyung Hee University)
Ryu, Hyeon Yeol (Korea Conformity Laboratories)
Song, Kyung Seuk (Korea Conformity Laboratories)
Lee, Jin Kyu (Korea Conformity Laboratories)
Lee, Sung Ryul (Department of Integrated Biomedical Science, Cardiovascular and Metabolic Disease Center, College of Medicine, Inje University)
Kang, Se Chan (Department of Oriental Medicine and Biotechnology, Kyung Hee University)
Publication Information
Toxicological Research / v.35, no.4, 2019 , pp. 371-387 More about this Journal
Abstract
Although the dried root of Saposhnikovia divaricata (Turcz.) Schischk. (Umbelliferae) is a popular medicinal plant in East Asia, there has been no systemic toxicological evaluation of a water extract of Saposhnikoviae Radix (SRE). In this experiment, an oral acute and 13-week subchronic toxicological evaluations of SRE (500-5,000 mg/kg body weight) were performed in both sexes of Crl:CD(SD) rats. Based on the results from mortality, clinical signs, effects on body weight and organ weight, clinical biochemistry, hematology, urinalysis, and histopathology, significant acute, 4-week repeated dose range finding (DRF) and 13-week subchronic toxicity of SRE was not observed in either sex of rats; thus, the no observed adverse effect level (NOAEL) was 5,000 mg (kg/day). To identify anti-hyperuricemia potential of SRE, the suppressive effect of SRE was determined in mice challenged with potassium oxonate (PO; 250 mg/kg) via intraperitoneal injection for 8 days (each group; n = 7). SRE supplementation suppressed the uric acid level in urine through significant xanthine oxidase (XO) inhibitory activity. Kidney dysfunctions were observed in PO-challenged mice as evidenced by an increase in serum creatinine level. Whereas, SRE supplementation suppressed it in a dose-dependent manner. Collectively, SRE was safe up to 5,000 mg (kg/day) based on NOAEL found from acute and 13-week subchronic toxicological evaluations. SRE had anti-hyperuricemia effect and lowered the excessive level of uric acid, a potential factor for gout and kidney failure.
Keywords
Saposhnikoviae radix; 13-week repeated toxicity; NOAEL; Potassium oxonate; Anti-hyperuricemia; Extract;
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