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http://dx.doi.org/10.5487/TR.2016.32.3.239

Propolis Inhibits Neurite Outgrowth in Differentiating SH-SY5Y Human Neuroblastoma Cells  

Kim, Han Bit (Dept. of Life Science, Kyonggi University)
Yoo, Byung Sun (Dept. of Life Science, Kyonggi University)
Publication Information
Toxicological Research / v.32, no.3, 2016 , pp. 239-243 More about this Journal
Abstract
Propolis is a multicomponent, active, complex resinous substance collected by honeybees from a variety of plant sources. We have studied the effect of propolis on neurite outgrowth of SH-SY5Y human neuroblastoma cells induced to differentiate by all-trans-retinoic acid (RA). Propolis, at a concentration of $3{\mu}g/mL$, had no significant effect on the viability of differentiating SH-SY5Y cells. However, the neurite outgrowth of the differentiating SH-SY5Y cells treated with propolis ($0.3{\sim}3{\mu}g/mL$) for 48 hr was significantly inhibited in a dose-dependent manner. Treatment of RA-stimulated differentiating SH-SY5Y cells with 0.3 to $3{\mu}g/mL$ propolis resulted in decreased level of transglutaminase and 43-kDa growth-associated protein (GAP-43) in a dose-dependent manner. The results indicate that propolis is able to inhibit neurite outgrowth of differentiating SH-SY5Y cells.
Keywords
Propolis; SH-SY5Y neuroblastoma cell; Neurite outgrowth; TGase; GAP-43;
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