Browse > Article
http://dx.doi.org/10.5487/TR.2016.32.3.225

Cytotoxicity Evaluation of Essential Oil and its Component from Zingiber officinale Roscoe  

Lee, Yongkyu (Department of Energy and Bio Engineering, Dongseo University)
Publication Information
Toxicological Research / v.32, no.3, 2016 , pp. 225-230 More about this Journal
Abstract
Zingiber officinale Roscoe has been widely used as a folk medicine to treat various diseases, including cancer. This study aims to re-examine the therapeutic potential of co-administration of natural products and cancer chemotherapeutics. Candidate material for this project, ${\alpha}$-zingiberene, was extracted from Zingiber officinale Roscoe, and ${\alpha}$-zingiberene makes up $35.02{\pm}0.30%$ of its total essential oil. ${\alpha}$-Zingiberene showed low $IC_{50}$ values, $60.6{\pm}3.6$, $46.2{\pm}0.6$, $172.0{\pm}6.6$, $80.3{\pm}6.6$ (${\mu}g/mL$) in HeLa, SiHa, MCF-7 and HL-60 cells each. These values are a little bit higher than $IC_{50}$ values of general essential oil in those cells. The treatment of ${\alpha}$-zingiberene produced nucleosomal DNA fragmentation in SiHa cells, and the percentage of sub-diploid cells increased in a concentration-dependent manner in SiHa cells, hallmark features of apoptosis. Mitochondrial cytochrome c activation and an in vitro caspase-3 activity assay demonstrated that the activation of caspases accompanies the apoptotic effect of ${\alpha}$-zingiberene, which mediates cell death. These results suggest that the apoptotic effect of ${\alpha}$-zingiberene on SiHa cells may converge caspase-3 activation through the release of mitochondrial cytochrome c into cytoplasm. It is considered that anti-proliferative effect of ${\alpha}$-zingiberene is a result of apoptotic effects, and ${\alpha}$-zingiberene is worth furthermore study to develop it as cancer chemotherapeutics.
Keywords
Cytotoxic activity; ${\alpha}$-Zingiberene; Anti-proliferation; General essential oil; Cervix cancer cells;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Fabian, D., Sabol, M., Domaracka, K. and Bujnakova, D. (2006) Essential oils - their antimicrobial activity against Escherichia coli and effect on intestinal cell viability. Toxicol. In Vitro, 20, 1435-1445.   DOI
2 Barnes, J. (2003) Quality, efficacy and safety of complementary medicines: fashions, facts and the future. Part I. regulation and quality. Br. J. Clin. Pharmacol., 55, 226-233.   DOI
3 Ali, B.H., Blunden, G., Tanira, M.O. and Nemmar, A. (2008) Some phytochemical, pharmacological and toxicological properties of ginger (Zingiber officinale Roscoe): a review of recent research. Food Chem. Toxicol., 46, 409-420.   DOI
4 Shukla, Y. and Singh, M. (2007) Cancer preventive properties of ginger: a brief review. Food Chem. Toxicol., 45, 683-690.   DOI
5 Vendruscolo, A., Takaki, I., Bersani-Amado, L.E., Dantas, J.A., Bersani-Amado, C.A. and Cuman, R.K. (2006) Antiinflammatory and antinociceptive activities of Zingiber officinale Roscoe essential oil in experimental animal models. Indian J. Pharmacol., 38, 58-59.   DOI
6 Carrasco, F.R., Schmidt, G., Romero, A.L., Sartoretto, J.L., Caparroz-Assef, S.M., Bersani-Amado, C.A. and Cuman, R.K. (2009) Immunomodulatory activity of Zingiber officinale Roscoe, Salvia officinalis L. and Syzygium aromaticum L. essential oils; evidence for humor- and cell-mediated responses. J. Pharm. Pharmacol., 61, 961-967.   DOI
7 Nogueira de Melo, G.A., Grespan, R., Fonseca, J.P., Farinha, T.O., da Silva, E.L., Romero, A.L., Bersani-Amado, C.A. and Cuman, R.K. (2011) Inhibitory effects of ginger (Zingiber officinale Roscoe) essential oil on leukocyte migration in vivo and in vitro. J. Nat. Med., 65, 241-246.   DOI
8 Lee, E. and Surh, Y.J. (1998) Induction of apoptosis in HL-60 cells by pungent vanilloids, [6]-gingerol and [6]-paradol. Cancer Lett., 134, 163-168.   DOI
9 Wei, Q.Y., Ma, J.P., Cai, Y.J., Yang, L. and Liu, Z.L. (2005) Cytotoxic and apoptopic activities of diarylheptanoids and gingerol-related compounds from the rhizomes of Chinese ginger. J. Ethnopharmacol., 102, 177-184.   DOI
10 Ishiguro, K., Ando, T., Maeda, O., Ohmiya, N., Niwa, Y., Kadomatsu, K. and Goto, H. (2007) Ginger ingredients reduce viability of gastric cancer cells via distinct mechanisms. Biochem. Biophys. Res. Commun., 362, 218-223.   DOI
11 Norajit, K., Laohakunjit, N. and Kerdchoechuen, O. (2007) Antibacterial effect of Zingiberaceae essential oils. Molecules, 23, 2047-2060.
12 Moon, H.I., Cho, S.B. and Kim, S.K. (2011) Composition and immunotoxicity activity of essential oils from leaves of Zingiber officinale Roscoe against Aedes aegypti L. Immunopharmacol. Immunotoxicol., 33, 201-204.   DOI
13 Sasidharan, I., Venugopal, V.V. and Menon, A.N. (2012) Essential oil composition of two unique ginger (Zingiber officinale Roscoe) cultivars from Sikkim. Nat. Prod. Res., 26, 1759-1764.   DOI
14 Yamamoto-Ribeiro, M.M., Grespan, R., Kohiyama, C.Y., Ferreira, F.D., Mossini, S.A., Silva, E.L., Filho, B.A., Mikcha, J.M. and Machinski, M. (2013) Effect of Zingiber officinale essential oil on Fusarium verticillioides and fumonisin production. Food Chem., 141, 3147-3152.   DOI
15 Khrimian, A., Shirali, S. and Guzman, F. (2015) Absolute configurations of zingiberenols isolated from Ginger (Zingiber officinale) rhizomes. J. Nat. Prod., 78, 3071-3074.   DOI
16 Adams, R.P. (2001) Identification of essential oil components by gas chromatography/quadrupole mass spectroscopy, Allured Publishing Corporation, Carol Stream, pp. 456-460.
17 Ji, M., Choi, J., Lee, J. and Lee, Y. (2004) Induction of apoptosis by ar-turmerone on various cell lines. Int. J. Mol. Med., 14, 253-256.
18 Kluck, R.M., Bossy-Wetzel, E., Green, D.R. and Newmeyer, D.D. (1997) The release of cytochrome c from mitochondria : a primary site for Bcl-2 regulation of apoptosis. Science, 275, 1132-1136.   DOI
19 Mohammad, A.M., Razieh, Y. and Mohammad, H.S. (2005) The cytotoxic and anti-proliferative effects of 3-hydrogenkwadaphin in K562 and Jurkat cells is reduced by guanosine. J. Biochem. Mol. Biol., 38, 391-398.   DOI
20 Finucanne, D.M., Bossy-Wetzel, E., Waterhouse, N.J., Cotter, T.G. and Green, D.R. (1999) Bax-induced caspase activation and apoptosis via cytochrome c release from mitochondria is inhibitable by Bcl-XL. J. Biol. Chem., 274, 2225-2233.   DOI
21 Kroemer, G., Dallaporta, B. and Resche-Rigon, M. (1998) The mitochondrial death/life regulator in apoptosis and necrosis. Annu. Rev. Physiol., 60, 619-642.   DOI
22 Agarwal, M., Walia, S., Dhingra, S. and Khambay, B.P. (2001) Insect growth inhibition, antifeedant and antifungal activity of compounds isolated/derived from Zingiber officinale Roscoe (ginger) rhizomes. Pest Manag. Sci., 57, 289-300.   DOI
23 Nogueira de Melo, G.A., Grespan, R., Fonseca, J.P., Farinha, T.O., da Silva, E.L., Romero, A.L., Bersani-Amado, C.A. and Cuman, R.K. (2011) Inhibitory effects of ginger (Zingiber officinale Roscoe) essential oil on leukocyte migration in vivo and in vitro. J. Nat. Med., 65, 241-246.   DOI
24 Bayala, B., Bassole, I.H., Gnoula, C., Nebie, R., Yonli, A., Morel, L., Figueredo, G., Nikiema, J.B., Lobaccaro, J.M. and Simpore, J. (2014) Chemical composition, antioxidant, antiinflammatory and anti-proliferative activities of essential oils of plants from Burkina Faso. PLoS ONE, 9, e92122.   DOI
25 Bartley, J.P. and Foley, P. (1994) Supercritical fluid extraction of australian-grown ginger (Zingiber officinale). J. Sci. Food Agric., 66, 365-371.   DOI
26 Hoferl, M., Stoilova, I., Wanner, J., Schmidt, E., Jirovetz, L., Trifonova, D., Stanchev, V. and Krastanov, A. (2015) Composition and comprehensive antioxidant activity of ginger (Zingiber officinale) essential oil from Ecuador. Nat. Prod. Commun., 10, 1085-1090.
27 Blair, J., Aichinger, T., Hackal, G., Hueber, K. and Dachler, M. (2001) Essential oil content and composition in commercially available dill cultivars in comparison to caraway. Ind. Crops Prod., 14, 229-239.   DOI
28 Galambosi, B. and Peura, P. (1996) Agrobotanical features and oil content of wild and cultivated forms of caraway (Carum carvi L). J. Essent. Oil Res., 8, 389-397.   DOI
29 Chen, W., Lu, Y., Gao, M., Wu, J., Wang, A. and Shi, R. (2011) Anti-angiogenesis effect of essential oil from Curcuma zedoaria in vitro and in vivo. J. Ethnopharmacol., 133, 220-226.   DOI
30 Charriaut-Marlangue, C., Margaill, I., Represa, A., Popovici, T., Plotkine, M. and Ben-Ari, Y. (1996) Apoptosis and necrosis after reversible focal ischemia: an in situ DNA fragmentation analysis. J. Cereb. Blood Flow Metab., 16, 186-194.   DOI
31 Ioannou, Y.A. and Chen, F.W. (1996) Quantitation of DNA fragmentation in apoptosis. Nucleic Acids Res., 24, 992-993.   DOI
32 Collins, J.A., Schandi, C.A., Young, K.K., Vesely, J. and Willingham, M.C. (1997) Major DNA fragmentation is a late event in apoptosis. J. Histochem. Cytochem., 45, 923-934.   DOI
33 Chen, S., Dobrovolsky, V.N., Liu, F., Wu, Y., Zhang, Z., Mei, N. and Guo, L. (2014) The role of autophagy in usnic acidinduced toxicity in hepatic cells. Toxicol. Sci., 142, 33-44.   DOI
34 Komada, Y., Zhang, X.L., Zhou, Y.W., Ido, M. and Azumam E. (1997) Apoptotic cell death of human T lymphoblastoid cells induced by arginine deiminase. Int. J. Hematol., 65, 129-141.   DOI
35 Rubio, V., Calvino, E., Garcia-Perez, A., Herraez, A. and Diez, J.C. (2014) Human acute promyelocytic leukemia NB4 cells are sensitive to esculetin through induction of an apoptotic mechanism. Chem. Biol. Interact., 220, 129-139.   DOI
36 Semisch, A., Ohle, J., Witt, B. and Hartwig, A. (2014) Cytotoxicity and genotoxicity of nano - and microparticulate copper oxide: role of solubility and intracellular bioavailability. Part Fibre Toxicol., 11, 10.   DOI
37 Sun, L.K., Yoshii, Y., Hyodo, A., Tsurushima, H., Saito, A., Harakuni, T., Li, Y.P., Kariva, K., Nozaki, M. and Morine, N. (2003) Apoptotic effect in the glioma cells induced by specific protein extracted from Okinawa Habu (Trimeresurus flavoviridis) venom in relation to oxidative stress. Toxicol. In Vitro, 17, 169-177.   DOI
38 Zong, B., Ma, Y., Fu, D. and Zhang, C. (2013) Induction of apoptosis in osteosarcoma s180 cells by polysaccharide from Dictyophora indusiata. Cell Biochem. Funct., 31, 719-723.   DOI
39 Ashkenazi, A. (2008) Directing cancer cells to self-destruct with pro-apoptotic receptor agonists. Nat. Rev. Drug Discov., 7, 1001-1012.   DOI
40 Elmore, S. (2007) Apoptosis: a review of programmed cell death. Toxicol. Pathol., 35, 495-516.   DOI
41 Rosse, T., Olivier, R., Monney, L., Rager, M., Conus, S., Fellay, I., Jansen, B. and Borner, C. (1998) Bcl-2 prolongs cell survival after Bax-induced release of cytochrome c. Nature 391, 496-499.   DOI
42 Earnshaw, W.C., Martins, L.M. and Kaufmann, S.H. (1999) Mammalian caspases: structure, activation, substrates, and functions during apoptosis. Annu. Rev. Biochem., 68, 383-424.   DOI
43 Decaudin, D., Marzo, I., Brenner, C. and Kroemer, G. (1998) Mitochondria in chemotherapy-induced apoptosis: a prospective novel target of cancer therapy. Int. J. Oncol., 12, 141-152.