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http://dx.doi.org/10.5487/TR.2012.28.3.151

Effects of Oxidized Tallow on the Rabbit Serum Lipids and Antioxidant Activity of the In-vitro Lipids  

Zeb, Alam (Department of Biotechnology, University of Malakand)
Rahman, Waheed Ur (Department of Biotechnology, University of Malakand)
Publication Information
Toxicological Research / v.28, no.3, 2012 , pp. 151-157 More about this Journal
Abstract
This paper describes the effects of thermally oxidized tallow on the serum lipids profile and radical scavenging activity (RSA) of the lipids extracted from the different tissues of the rabbits. Tallow was thermally oxidized at $130^{\circ}C$ for 9, 18, 27, 36 and 45 h respectively. Thermally oxidized tallow was fed to the local strain of Himalayan rabbits for one week. Results show that oxidation increases the formation of hydroperoxides and decrease the level of radical scavenging activity of the tallow. The rabbit serum lipids profile showed a dose dependent increase in triglyceride, total cholesterol and LDL-cholesterol. However, no statistically significant increase was observed in the HDL-cholesterol with an increase of oxidation time. Serum glucose and rabbits body weight decrease significantly (p < 0.05) and was highly correlated with the serum lipids profile. The percent RSA of the lipids extracted from the liver, brain and muscles tissues showed a significant decrease with respect to 0.5, 1.0 and 1.5 g/body weight as well as oxidation time. Data suggests that thermal oxidation and use of thermally oxidized beef tallow is harmful and therefore an alternative way of cooking should be used.
Keywords
Tallow; Thermal oxidation; Rabbit; Serum lipids profile; Radical scavenging activity;
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1 Jurek, D., Udilova, N., Jozkowicz, A., Nohl, H., Marian, B. and Schulte-Hermann, R. (2005). Dietary lipid hydroperoxides induce expression of vascular endothelial growth factor (VEGF) in human colorectal tumor cells. FASEB J., 19, 97-99.   DOI
2 Zullo, B.A. and Ciafardini, G. (2008). The olive oil oxygen radical absorbance capacity (DPPH assay) as a quality indicator. Eur. J. Lipid Sci. Technol., 110, 428-434.   DOI   ScienceOn
3 Ramadan, M.F., Amer, M.M.A. and Sulieman, A.R.M. (2006). Correlation between physicochemical analysis and radical-scavenging activity of vegetable oil blends as affected by frying of French fries. Eur. J. Lipid Sci. Technol., 108, 670-678.   DOI   ScienceOn
4 Yang, C.M., Kendall, C.W., Stamp, D., Medline, A., Archer, M.C. and Bruce, W.R. (1998). Thermally oxidized dietary fat and colon carcinogenesis in rodents. Nutr. Cancer, 30, 69-73.   DOI   ScienceOn
5 Ryan, T.C. and Gray, J.I. (1984). Distribution of cholesterol in fractionated beef tallow. J. Food Sci., 49, 1390-1391.   DOI
6 Sabir, S.M., Hayat, I. and Gardezi, S.D.A. (2003). Estimation of sterols in edible fats and oils. Pak. J. Nutr., 2, 178-181.   DOI
7 Segura, N., Silva, R.C., Soares, F.A.S.M., Gioielli, L.A. and Jachmanián, I. (2011). Valorization of beef tallow by lipase-catalyzed interesterification with high oleic sunflower oil. J. Am. Oil Chem. Soc., 88, 1945-1954.   DOI   ScienceOn
8 Yuan, Y.V., Kitts, D.D. and Godin, D.V. (1999). Influence of increased saturated fatty acid intake from beef tallow on antioxidant status and plasma lipids in atherosclerosis-susceptible Japanese quail. Nutr. Res., 19, 461-481.   DOI   ScienceOn
9 Zeb, A. (2012). Thermal degradation of $\beta$-carotene in food oils. (Preedy, V.R., Ed). Royal Society of Chemistry, London, England.
10 Zeb, A. and Ali, M. (2008). Thermal stability of animal tallow used in kebab preparation. J. Chem. Soc. Pak., 30, 750-755.
11 Zeb, A. and Mehmood, A. (2012). Effects of oxidized vanaspati ghee on the serum lipids and radical scavenging activity of the in-vitro lipids of liver, brain and muscles. Turk. J. Biochem., (In Press).
12 Zeb, A. and Murkovic, M. (2010). Characterization of the effects of $\beta$-carotene on the thermal oxidation of triacylglycerols using HPLC-ESI-MS. Eur. J. Lipid Sci. Technol., 112, 1218-1228.   DOI   ScienceOn
13 Lechowski, R., Bielecki, W., Sawosz, E., Krawiec, M. and Klucinski, W. (1999). The effect of lecithin supplementation on the biochemical profile and morphological changes in the liver of rats fed different animal fats. Vet. Res. Commun., 23, 1-14.   DOI   ScienceOn
14 Kamal-Eldin, A., Makinen, M. and Lampi, A. (2003). The challenging contribution of hydroperoxides to the lipid oxidation mechanism (Kamal-Eldin, A., Ed). AOCS Press, Champaign, Illinois, pp. 1-35.
15 Karabulut, I. (2010). Effects of $\alpha$-tocopherol, $\beta$-carotene and ascorbyl palmitate on oxidative stability of butter oil triacylglycerols. Food Chem., 123, 622-627.   DOI   ScienceOn
16 Kehrer, J.P. (1993). Free radicals as mediator of tissue injury and disease. Crit. Rev. Toxicol., 23, 21-48.   DOI   ScienceOn
17 Lee, J.M., Chung, H., Chang, P.S. and Lee, J.H. (2007). Development of a method predicting the oxidative stability of edible oils using 2,2-diphenyl-1-picrylhydrazyl (DPPH). Food Chem., 103, 662-669.   DOI   ScienceOn
18 Leplaix-Charlat, L., Durand, D. and Bauchart, D. (1996). Effects of diets containing tallow and soybean oil with and without cholesterol on hepatic metabolism of lipids and lipoproteins in the pre ruminant calf. J. Dairy Sci., 79, 1826-1835.   DOI   ScienceOn
19 Mai, J., Tsai, C.H., Armbruster, G., Chu, P. and Kinsella, J.E. (1980). Effects of microwave cooking on food fatty acids: No evidence of chemical alteration or isomerization. J. Food Sci., 45, 1753-1755.   DOI
20 Pour-Reza, J. and Edriss, M.A. (1997). Effects of dietary sorghum of different tannin concentrations and tallow supplementation on the performance of broiler chicks. Br. Poult. Sci., 38, 512- 517.   DOI   ScienceOn
21 Berger, J.J. and Barnard, R.J. (1999). Effect of diet on fat cell size and hormone-sensitive lipase activity. J. Appl. Physiol., 87, 227-232.   DOI
22 Achir, N., Randrianatoandro, V.A., Bohuon, P., Laffargue, A. and Avallone, S. (2010). Kinetic study of $\beta$-carotene and lutein degradation in oils during heat treatment. Eur. J. Lipid Sci. Technol., 112, 349-361.
23 Ali, M., Ullah, I., Ahmad, S., Khan, H. and Akbar, H. (2009). Effect of commercial kebab frying on physico-chemical parameters of the tallow. Pak. J. Nutr., 8, 891-895.   DOI
24 AOCS. (1998). Official methods and recommended practices of the American oil chemists' society, D. Firestone (5th ed.). Champaign: AOCS. Method Cd 8b-90.
25 Folch, J., Lees, M. and Sloane Stanley, G.H. (1957). A simple method for isolation and purification of total lipids from animal tissues. J. Biol. Chem., 226, 497-509.
26 Guderian, Jr., D.M., Rasmussen, H.E., Wray, C.A., Dussault, P.H. and Carr, T.P. (2007). Cholesterol-lowering properties of plant sterols esterified with beef tallow fatty acids in hamsters. Nutr. Res., 27, 283-288.   DOI   ScienceOn