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http://dx.doi.org/10.5487/TR.2011.27.4.191

Toxicological Characterization of Phthalic Acid  

Bang, Du-Yeon (Laboratory of Toxicology, College of Pharmacy, Sungkyunkwan University)
Lee, In-Kyung (Laboratory of Toxicology, College of Pharmacy, Sungkyunkwan University)
Lee, Byung-Mu (Laboratory of Toxicology, College of Pharmacy, Sungkyunkwan University)
Publication Information
Toxicological Research / v.27, no.4, 2011 , pp. 191-203 More about this Journal
Abstract
There has been growing concern about the toxicity of phthalate esters. Phthalate esters are being used widely for the production of perfume, nail varnish, hairsprays and other personal/cosmetic uses. Recently, exposure to phthalates has been assessed by analyzing urine for their metabolites. The parent phthalate is rapidly metabolized to its monoester (the active metabolite) and also glucuronidated, then excreted. The objective of this study is to evaluate the toxicity of phthalic acid (PA), which is the final common metabolic form of phthalic acid esters (PAEs). The individual PA isomers are extensively employed in the synthesis of synthetic agents, for example isophthalic acid (IPA), and terephthalic acid (TPA), which have very broad applications in the preparation of phthalate ester plasticizers and components of polyester fiber, film and fabricated items. There is a broad potential for exposure by industrial workers during the manufacturing process and by the general public (via vehicle exhausts, consumer products, etc). This review suggests that PA shows in vitro and in vivo toxicity (mutagenicity, developmental toxicity, reproductive toxicity, etc.). In addition, PA seems to be a useful biomarker for multiple exposure to PAEs in humans.
Keywords
Phthalic acid; Phthalates; Common metabolite; Plasticizer;
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1 Verrett, M.J., Mutchler, M.K., Scott, W.F., Reynaldo, E.F. and McLaughlin. (1969). Teratogenic effects of captan and related compounds in the developing chicken embryo. Ann. N. Y. Acad. Sci., 160, 334-343.   DOI
2 Williams, D.T. and Blanchfield, B.J. (1974). Retention, excretion, and metabolism of phthalic acid administered orally to the rat. Bull. Environ. Contam. Toxicol., 12, 109-112.   DOI
3 Wolkowski-Tyl, R., Chin, T.Y. and Heck, H.D. (1982). Chemical urolithiasis. III. Pharmacokinetics and transplacental transport of terephthalic acid in Fischer-344 rats. Drug. Metab. Dispos., 10, 486-490.
4 Wolkowski-Tyl, R. and Chin, T.Y. (1983). Effects of selected therapeutic agents on urolithiasis induced by terephthalic acid in the male weanling Fischer 344 rat. Fundam. Appl. Toxicol., 3, 552-558.   DOI   ScienceOn
5 Silva, M.J., Malek, N.A., Hodge, C.C., Reidy, J.A., Kato, K., Barr, D.B., Needham, L.L. and Brock, J.W. (2003). Improved quantitative detection of 11 urinary phthalate metabolites in humans using liquid chromatography-atmospheric pressure chemical ionization .tandem mass spectrometry. J. Chromatogr. B, 789, 393-404.   DOI   ScienceOn
6 Silva, M.J., Barr, D.B., Reidy, J.A., Malek, N.A., Hode, C.C., Caudill, S.P., Brock, J.W., Needham, L.L. and Calafat, A.M. (2004). Urinary levels of seven phthalate metabolites in the U.S. population from the National Health and Nutrition Examination Survey (NHANES) 1999-2000. Environ. Health Perspect., 112, 331-338.
7 Thomas, J.A. and Thomas, M.J. (1984). Biological effects of di-(2-ethylhexyl) phthalate and other phthalic acid esters. Crit. Rev. Toxicol., 13, 283-317.   DOI   ScienceOn
8 Silva, M.J., Reidy, J.A., Samandar, E., Herbert, A.R., Needham, L.L. and Calafat, A.M. (2005). Detection of phthalate metabolites in human saliva. Arch. Toxicol., 79, 647-652.   DOI
9 Staples, C.A., Peterson, D.R., Parkerton, T.F. and Adams, W.J. (1997). The environmental fate of phthalate esters: a literature review. Chemosphere, 35, 667-749.   DOI   ScienceOn
10 Tavares, I.A. and Vine, N.D. (1985). Phthalic acid esters inhibit arachidonate metabolism by rat peritoneal leucocytes. J. Pharm. Pharmacol., 37, 67-68.   DOI
11 TOLED5. (1997). (Elsevier Science Pub.B.V., POB211, 1000AE Amsterdam, Netherlands) V.1- 1977-. 93, 109.   DOI   ScienceOn
12 http://www.ccohs.ca/products/databases/samples/rtecs.html
13 Vermeulen, R., Jonsson, B.A., Lindh, C.H. and Kromhout, H. (2005). Biological monitoring of carbon disulphide and phthalate exposure in the contemporary rubber industry. Int. Arch. Occup. Environ. Health, 78, 663-669.   DOI
14 Price, K.E. and Zolli, Z. (1958). The influence of terephthalic acid on oxytetracycline serum levels in chicken studies on mode of action. I. Avian Diseases, 157, 157-169.
15 Raisz, L.G. (1995). Physiologic and pathologic roles of prostagladins and other eicosanoids in bone metabolism. J. Nutr., 125, 2024S-2027S.   DOI
16 Sharman, M., Read, W.A., Castle, L. and Gilbert, J. (1994). Levels of di-(2-ethylhexyl)phthalate and total phthalate esters in milk, cream, butter and cheese. Food Addit. Contam., 11, 375-385.   DOI   ScienceOn
17 Raisz, L.G., Fall, P.M., Gabbitas, B.Y., McCarthy, T.L., Kream, B.E. and Canalis, E. (1993). Effects of prostaglandin E2 on bone formation in cultured fetal rat calvariae: role of insulinlike growth factor-1. Endocrinology, 133, 1504-1510.   DOI   ScienceOn
18 Richburg, J.H. and Boekelheide, K. (1996). Mono-(2-ethylhexyl) phthalate rapidly alters both Sertoli cell vimentin filaments and germ cell apoptosis in young rat testes. Toxicol. Appl. Pharmacol., 137, 42-50.   DOI   ScienceOn
19 Roth, V.S. (1999). Rubber industry epidemiology. Occup. Med., 14, 849-856.
20 Sharpe, R.M. (1998). Natural and anthropogenic environmental oestrogens: the scientific basis for risk assessment. Pure Appl. Chem., 70, 1685-1701.   DOI   ScienceOn
21 Shemiakin, M.M. and Schukina, L.A. (1944). Experimental corroboration of the mechanism of biological action of quinones of the type of vitamin K. Nature, 154, 513.   DOI
22 Shintani, H. (1985). Determination of phthalic acid, mono-(2-ethylhexyl) phthalate and di-(2-ethylhexyl)phthalate in human plasma and in blood products. J. Chromatogr., 337, 279-290.   DOI   ScienceOn
23 Okubo, T., Suzuki, T., Yokoyama, Y., Kano, K. and Kano, I. (2003). Estimation of estrogenic and anti-estrogenic activities of some phthalate diesters and monoesters by MCF-7 cell proliferation assay in vitro. Biol. Pharm. Bull., 26, 1219-1224.   DOI   ScienceOn
24 Perteson, E.H., Hendrix, W.L. and Braddy.L.D. (1959). The potentiation of erythromycin and oxytetracycline activity in birds by means of terephthalic acid. Poultry Sci., 38, 235-237.   DOI
25 Parks, L.G., Ostby, J.S., Lambright, C.R., Abbott, B.D., Klinefelter, G.R., Barlow, N.J. and Gray, L.E. (2000). The plasticizer diethylhexyl phthalate induces malformations by decreasing fetal testosterone synthesis during sexual differentiation in the male rat. Toxicol. Sci., 58, 339-349.   DOI   ScienceOn
26 Pauli, G., Bessot, J.C., Kopferschmitt, M.C., Lingot, G., Wending, R., Ducos, P. and Limasset, J.C. (1980). Meat wrapper's asthma: identification of the causal agent. Clin. Allergy, 10, 263-269.   DOI
27 Pavan, B., Biondi, C., Ferretti, M.E., Lunghi, L. and Paganetto, G. (2001). Phthalic acid mimics 17beta-estradiol actions in WISH cells. Toxicol. Lett., 118, 157-164.   DOI   ScienceOn
28 Pfaffli, P. (1986). Phthalic acid excretion as an indicator of exposure to phthalic anhydride in the work atmosphere. Int. Arch. Occup. Environ. Health, 58, 209-216.   DOI
29 Phillips, B.J., James, T.E. and Gangolli, S.D. (1982). Genotoxicity studies of di(2-ethylhexyl)phthalate and its metabolites in CHO cells. Mutat. Res., 102, 297-304.   DOI   ScienceOn
30 Pilbeam, C.C., Raisz, L.G., Voznesensky, O., Alander, C.B., Delman, B.N. and Kawaguchi, H. (1995). Autoregulation of inducible prostaglandin G/H synthase in osteoblastic cells by prostaglandins. J. Bone Miner. Res., 10, 406-414.
31 Moffitt, A.E., Clary, J.J., Lewis, T.R., Blanck, M.D. and Perone, V.B. (1975). Absorption, distribution, and excretion of terePA and dimethylterephthalate. Amer. Ind. Hyg. Assoc. J., 36, 633-641.   DOI   ScienceOn
32 NIOSH, National Institute of Occupational Safety and Health. (1981). Occupational health guideline for phthalic anhydride. DHHS(NIOSH) Publication No 81-123, US Department of Labor. Occupational Safety and Health Administration.
33 Mylchreest, E., Cattley, R.C. and Foster, P.M.D. (1998). Male reproductive tract malformations in rats following gestational and lactational exposure to di(n-butyl) phthalate: an antiandrogenic mechanism? Toxicol. Sci., 43, 47-60.   DOI
34 Mylchreest, E., Sar, M., Cattley, R.C. and Foster, P.M.D. (1999). Disruption of androgen-regulated male reproductive development by di(n-butyl) phthalate during late gestation in rats is different from flutamide. Toxicol. Appl. Pharmacol., 156, 81-95.   DOI   ScienceOn
35 Mylchreest, E., Wallace, D.G., Cattley, R.C. and Foster, P.M.D. (2000). Dose-dependent alterations in androgen-regulated male reproductive development in rats exposed to di(n-butyl) phthalate during late gestation. Toxicol. Sci., 55, 143-151.   DOI   ScienceOn
36 Needham, L.L., Patterson, D.G., Barr, D.B., Grainger, J. and Calafat, A.M. (2005). Uses of speciation techniques in biomonitoring for assessing human exposure to organic environmental chemicals. Anal. Bioanal. Chem., 381, 397-404.   DOI
37 Oishi, S. and Hiraga, K. (1980). Testicular Atrophy Induced by phthalic acid Esters: Effect on Testosterone and Zinc Concentrations. Toxicol. Appl. Pharmacol., 53, 35-41.   DOI   ScienceOn
38 Oishi, S. and Hiraga, K. (1982). Effects of Monoesters of Ophthalic acid on serum lipid composition of rats. Toxicol. Lett., 14, 79-84.   DOI   ScienceOn
39 Kawaguchi, H., Pilbeam, C.C., Harrison, J.R. and Raisz, L.G. (1995). The role of prostaglandins in the regulation of bone metabolism. Clin. Orthop. Relat. Res., 313, 36-46.
40 Li, Z., Zhang, C., Wang, K., Gu, L., Shi, A., Gong, N., Xu, X., He, D., Xu, J. and Wang, X. (1999). [Changes in the pulmonary function of factory workers exposure to terephthalic acid]. Wei Sheng Yan Jiu, 28, 1-3.
41 Maccia, C.A., Bernstein, I.L., Emmet, E.A. and Broaks, S.M. (1976). In vitro demonstration of specific IgE in phthalic anhydride hypersensitivity. Am. Rev. Resp. Disease, 113, 701-704.
42 Marhold, J. (1986). Preheld Prumyslove Toxikologie; Organicke Latky. Prague, Czechoslovakia, Avecenum, 317.
43 Masui, T., Shirai, T., Imaida, K., Uwagawa, S. and Fukushima, S. (1988). Effects of urinary crystals induced by acetazolamide, uracil, and diethylene glycol on urinary bladder carcinogenesis in N-butyl-N-(4-hydroxybutyl)nitrosamine-initiated rats. Toxicol. Lett., 40, 119-126.   DOI   ScienceOn
44 Mayer, F.L., Stalling, D.L. and Johnson, J.L. (1972). Phthalate esters as environmental contaminants. Nature, 238, 411-413.   DOI   ScienceOn
45 McLachlan, J.A. (2001). Environmental signaling: what embryos and evolutionteach us about endocrine disrupting chemicals. Endocr. Rev., 22, 319-341.   DOI   ScienceOn
46 Mettang, T., Alscher, D.M., Pauli-Magnus, C., Dunst, R., Kuhlmann, U. and Rettenmeier, A.W. (1999). Phthalic acid is the main metabolite of the plasticizer di(2-ethylhexyl) phthalate in peritoneal dialysis patients. Adv. Perit. Dial., 15, 229-233.
47 Mettang, T., Pauli-Magnus, C., Alscher, D.M., Kirchgessner, J., Wodarz, R., Rettenmeier, A.W. and Kuhlmann, U. (2000). Influence of plasticizer-free CAPD bags and tubings on serum, urine, and dialysate levels of phthalic acid esters in CAPD patients. Perit. Dial. Int., 20, 80-84.
48 Huber, W.W., Grasl-Kraupp, B. and Schulte-Hermann, R. (1996). Hepatocarcinogenic potential of di(2-ethylhexyl)phthalate in rodents and its implications on human risk. Crit. Rev. Toxicol., 26, 365-481.   DOI
49 Hoshi, A. and Kuretani, K. (1968). Distribution of terephthalic acid in tissues. Chem. Pharm. Bull., 16, 131-135.   DOI   ScienceOn
50 Hoppin, J.A., Brock, J.W., Davis, B.J. and Baird, D.D. (2002). Reproducibility of urinary phthalate metabolites in first morning urine samples. Environ. Health Perspect., 110, 515-518.   DOI
51 IITRI, IIT Research Institute. (1988). Four-week Inhalation Toxicity Study of Isophthalic acid in Rats. Study No. 1301.
52 IITRI, IIT Research Institute. (1990). Acute Oral Toxicity Study of Isophthalic acid in Rats. Study No. 1553.
53 Industrial BIO-TEST Lab. Inc. (1958). Report to Amoco Corporation Range-Finding Toxicity Studies on There Test Materials.
54 Jaeger, R.J. and Rubin, R.J. (1970a). Plasticizers from plastic devices : Excretion, metabolism and accumulation by biological systems. Science, 178, 460-462.
55 Jaeger, R.J. and Rubin, R.J. (1970b). Contamination of blood stored in plastic packs. Lancet, 2, 151.
56 Japan Environment Agency. (1998). Strategic Programs on Environmental Endocrine Disruptors '98 (SPEED '98). http://www.env.go.jp/en/chemi/ed/speed98/sp98.html.
57 Jha, A.M., Singh, A.C. and Bharti, M. (1998). Germ cell mutagenicity of phthalic acid in mice. Mutat. Res., 422, 207-212.   DOI   ScienceOn
58 Gibson, J.E. (1982). A ninety day study of terephthalic acidinduced urolithiasis and reproductive performance in Wistar and CD rats. Research Triangle Institute Experimental Pathology Laboratories, Inc. Chemical Industry Institute of Toxicology.
59 Kato, K., Silva, M.J., Needham, L.L. and Calafat, A.M. (2005). Determination of total phthalates in urine by isotope-dilution liquid chromatography-tandem mass spectrometry. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 814, 355-360.   DOI   ScienceOn
60 Gray, T.J. and Beamand, J.A. (1984). Effect of some phthalate esters and other testicular toxins on primary cultures of testicular cells. Food Chem. Toxicol., 22, 123-131.   DOI   ScienceOn
61 GISAAA. (1967). Gigiena i Sanitariya. For English translation. See HYSAAV. 32, 12.
62 Grigas, E.O., Ruiz, R. and Ariado, D.M. (1971). Cardiopulmonary effects of antimalarial drugs. IV. Terephthalic acid and its dihydroxamic derivative. Toxicol. Appl.Pharmacol., 18, 469-486.   DOI   ScienceOn
63 Guess, W.L., Jacob, J. and Autian, J. (1967). A study of polyvinyl chloride blood bag assemblies I Alteration or contamination of ACD solutions. Drug Intelligence, 1, 120-127.   DOI
64 Hall, I.H., Wong, O.T., Reynolds, D.J., Simlot, R. and Chang, J.J. (1993). Terephthalic Acid in Sprague-Dawley rats as a hypolipidemic agent. Arch. Pharm., 326, 5-13.   DOI
65 Heindel, J.J. and Powell, C.J. (1992). Phthalate ester effects on rat Sertoli cell function-in vitro-effects of phthalate side-chain and age of animal. Toxicol. Appl. Pharmacol., 115, 116-123.   DOI   ScienceOn
66 Hoshi, A., Yanai, R. and Kuretani, K. (1967). Effect of terephthalic acid upon the sulfadimethoxine content of blood plasma. Chem. Pharm. Bull., (Tokyo), 15, 1138-1144.   DOI   ScienceOn
67 Duty, S.M., Silva, M.J., Barr, D.B., Brock, J.W., Ryan, L., Chen, Z., Herrick, R.F., Christiani, D.C. and Hauser, R. (2003). Phthalate exposure and human semen parameters. Epidemiology, 14, 269-277.
68 Hoshi, A. and Kuretani, K. (1967). Metabolism of terephthalic acid. 3. Absorption of terephthalic acid from the gastrointestinal tract and detection of its metabolites. Chem. Pharm. Bull., 15, 1979-1984.   DOI   ScienceOn
69 Hoshi, A., Yanai, R. and Kuretani, K. (1968).Toxicity of terephthalic acid. Chem. Pharm. Bull., 16, 1655-1660.   DOI   ScienceOn
70 Cui, L., Shi, Y., Dai, G., Pan, H., Chen, J., Song, L., Wang, S., Chang, H.C., Sheng, H. and Wang, X. (2006). Modification of N-Methyl-N-Nitrosourea initiated bladder carcinogenesis in Wistar rats by terephthalic acid. Toxicol. Appl. Pharmacol., 210, 24-31.   DOI   ScienceOn
71 Ema, M., Miyawaki, E., Harazono, A. and Kawashima, K. (1997). Developmental toxicity evaluation of phthalic acid, one of the metabolites of phthalic acid esters, in rats. Toxicol. Lett., 93, 109-115.   DOI   ScienceOn
72 Ema, M. and Miyawaki, E. (2001). Effects of monobutyl phthalate on reproductive function in pregnant and pseudopregnant rats. Reprod. Toxicol., 15, 261-267.   DOI   ScienceOn
73 Ema, M. and Miyawaki, E. (2002). Effects on development of the reproductive system in male offspring of rats given butyl benzyl phthalate during late pregnancy. Reprod. Toxicol., 16, 71-76.   DOI   ScienceOn
74 Ema, M., Miyawaki, E., Hirose, A. and Kamata, E. (2003). Decreased anogenital distance and increased incidence of undescended testes in fetuses of rats given monobenzyl phthalate, a major metabolite of butyl benzyl phthalate. Reprod. Toxicol., 17, 407-412.   DOI   ScienceOn
75 Boekelheide, K. (1993). Sertoli cell toxicants. In The Sertoli Cell (L.D. Russell and M.D. Griswold, Eds.), p.551-575. Cache River Press, Clearwater, FL.
76 Forsberg, K., et al. (1997). Quick selection guide to chemical protective clothing. 3rd ed. Van Nostrand Reinhold.
77 Gray, L.E., Ostby, J., Furr, J., Price, M., Veeramachaneni, D.N.R. and Parks, L. (2000). Perinatal exposure to the phthalates DEHP, BBP, and DINP, but not DEP, DMP, or DOTP, alters sexual differentiation of the male rat. Toxicol. Sci., 58, 350-365.   DOI   ScienceOn
78 Blount, B.C., Silva, M.J., Caudill, S.P., Needham, L.L., Pirkle, J.L., Sampson, E.J., Lucier, G.W., Jackson, R.J. and Brock, J.W. (2000). Levels of seven urinary phthalate metabolites in a human reference population. Environ. Health Perspect., 108, 979-982.
79 Boots, S.G., Franklin, M.A., Dunlavey, B., Costello, J., Lipsitz, C., Boots, M.R. and Rogers, K.S. (1976). Synthesis of 5-substituted isoPAs and competitive inhibition studies with bovine liver glutamate dehydrogenase. Proc. Soc. Exp. Biol. Med., 151, 316-320.   DOI
80 Calandra, J.C. (1975). IBT No. 601-06339 - Acute Toxicity Studies with Five Test Materials - P.O.No. 99-8-4140. Industrial BIO-TEST Laboratories, Inc., Northbrook, IL.
81 Carozzi, S., Nasini, M.G., Schelotto, C., Caviglia, P.M., Santoni, O. and Pietrucci, A. (1993). A biocompatibility study on peritoneal dialysis solution bags for CAPD. Adv. Peritoneal Dial., 9, 138-142.
82 Chin, T.Y., Tyl, R.W., Popp, J.A. and Heck, H.D. (1981). Chemical urolithiasis: 1. Characteristics of bladder stone induction by terePA and dimethyl terephthalate in weanling Fischer-344 rats. Toxicol. Appl. Pharmacol., 58, 307-321.   DOI   ScienceOn
83 CIIT. (1983). Chronic Dietary Administration of TerePA. CIIT Docket 20124.
84 Amoco Co. (1990) Acute Oral Toxicity Study of TerePA in Rats. Conducted by IIT Research Institute. IITRI Study #1557.
85 Chester, E.H., Schwartz, H.J., Payne, C.B. Jr. and Greenstein, S. (1977). Phthalic anhydride asthma. Clin. Allergy, 7, 15-20.   DOI
86 Cooper, R.L. and Kavlock, R.J. (1997). Endocrine disruptors and reproductive development: a weight-of-evidence overview. J. Endocrinol., 152, 159-166.   DOI   ScienceOn
87 Amoco Co. (1975). Acute Oral Toxicity Study of TerePA in Rats. Conducted by Industrial Bio-Test Laboratories, Inc. IBT Study #601-06339.
88 ATSDR. (1995). Toxicological profile for diethyl phthalate (DEP). Agency for Toxic Substances and Disease Registry, Atlanta, GA. Available online via. http://www.atsdr.cdc.gov/toxpro.les.
89 ATSDR. (1997). Toxicological profile for di-n-octyl phthalate (DNOP). Agency for Toxic Substances and Disease Registry, Atlanta, GA. Available online via. http://www.atsdr.cdc.gov/toxpro.les/tp95.html.
90 ATSDR. (2000). Toxicological pro.le for di(2-ethylhexyl)phthalate (DEHP).. U.S. Department of Health and Human Services, Public Health Service, ATSDR, Atlanta GA. Available online via http://www.atsdr.cdc.gov/toxpro.les/.
91 ATSDR. (2001). Toxicological profile for di-n-butyl phthalate (DBP). Agency for Toxic Substances and Disease Registry, Atlanta, GA.
92 ATSDR. (2002). Toxicological Profile for Di(2-ethylhexyl)phthalate (DEHP). Atlanta, GA : Agency for Toxic Substances and Disease Registry, Atlanta, GA.
93 Barr, D.B., Silva, M.J., Kato, K., Reidy, J.A., Malek, N.A., Hurtz, D., Sadowski, M., Needham, L.L. and Calafat, A.M. (2003). New directions in the quantitation of human exposure to phthalates. Environ. Health. Perspect., Online, 24 February 2003, DOI 10.1289/ ehp.6074.
94 Albro, P.W., Tomas, R. and Fishbein, L. (1973). Metabolism of diethylhexyl phthalate by rats. Isolation and characterization of the urinary metabolites. J. Chromatogr., 76, 321-330.   DOI   ScienceOn
95 Bauer, M.J. and Herrmann, R. (1997). Estimation of the environmental contamination by phthalic acid esters leaching from household wastes. Sci. Total Environ., 208, 49-57.   DOI   ScienceOn
96 BIBRA working group. (1989). Ortho-PA and its sodium and potassium salts. Toxicity profile. The British Industrial Biological Research Association. p.5.
97 Agarwal, D.K., Lawrence, W.H., Nunez, L.J. and Autian, J. (1985). Mutagenicity evaluation of phthalic acid esters and metabolites in Salmonella typhimurium cultures. J. Toxicol. Environ. Health, 16, 61-69.   DOI   ScienceOn
98 Albro, P.W. and Thomas, R.O. (1973). Enzymatic hydrolysis of di-(2-ethylhexyl) phthalate by lipases. Biochim. Biophys. Acta. Biochim., 306, 380-390.   DOI   ScienceOn
99 Albro, P.W. and Moore, B. (1974). Identification of the metabolites of simple phthalate diesters in rat urine. J. Chromatogr., 94, 209-218.   DOI
100 Albro, P.W., Jordan, S., Corbett, J.T. and Schroeder, J.L. (1984). Determination of total phthalate in urine by gas chromatography. Anal. Chem., 56, 247-250.   DOI   ScienceOn
101 Albro, P.W. (1987). The biochemical toxicology of di-(2-ethylhexyl) and related phthalates: Testicular atrophy and hepatocarcinogenesis. Rev. Biochem. Toxicol., 8, 73-119.
102 Amoco Co. (1970). Fifteen Week Oral Toxicity of TerePA. Albino Rats. Conducted by Toxicological Evaluations. LSL Study#1358.