Browse > Article
http://dx.doi.org/10.5487/TR.2010.26.1.053

Mouse Single Oral Dose Toxicity Study of DHU001, a Polyherbal Formula  

Roh, Seong-Soo (Dept. of Herbology, College of Oriental Medicine, Daegu Haany University)
Ku, Sae-Kwang (Development Anatomy and Histology, College of Oriental Medicine, Daegu Haany University)
Publication Information
Toxicological Research / v.26, no.1, 2010 , pp. 53-59 More about this Journal
Abstract
This study was conducted to obtain acute information of the oral dose toxicity of DHU001, a polyherbal formula in male and female mice. In order to calculated 50% lethal dose ($LD_{50}$) and approximate lethal dose (LD), test material was once orally administered to male and female ICR mice at dose levels of 2000, 1000, 500, 250 and 0 (vehicle control) ml/kg (body weight). The mortality and changes on body weight, clinical signs, gross observation, organ weight and histopathology of principle organs were monitored 14 days after treatment with DHU001. We could not find any mortalities, DHU001 treatment-related clinical signs, changes on the body and organ weights, gross and histopathological findings. The results obtained in this study suggest that $LD_{50}$ and approximate LD in mice after single oral dose of DHU001 were considered over 2000 mg/kg in both female and male mice.
Keywords
DHU001; Polyherbal formula; Single oral dose toxicity; Mice; Histopathology;
Citations & Related Records
Times Cited By KSCI : 6  (Citation Analysis)
연도 인용수 순위
1 Narimanian, M., Badalyan, M., Panosyan, V., Gabrielyan, E., Panossian, A., Wikman, G. and Wagner, H. (2005). Impact of Chisan (ADAPT-232) on the quality-of-life and its efficacy as an adjuvant in the treatment of acute non-specific pneumonia. Phytomedicine, 12, 723-729.   DOI   ScienceOn
2 Organization for Economic Co-Operation and Development (Ed.). OECD guideline (423) for testing of chemicals - acute oral toxicity- acute toxic class method. 2001.
3 Park, J.H. and Geon, D.G. (2003). Pharmacognostical studies on the Chinese crude drug "Maig Moon Dong". Kor. J. Pharmacogn., 34, 6-9.   과학기술학회마을
4 Pineda, M.H. (1989). Female reproductive system in Veterinary endocrinology and reproduction (McDonald, L.E. and Pineda, M.H. Eds.). Lea & Febiger, Philadelphia, pp. 303-354.
5 Plata, E.J. and Murphy, W.H. (1972). Growth and haematologic properties of the BALB/wm strain of inbred mice. Lab. Anim. Sci., 22, 712-720.
6 Rhyu, M.R., Kim, E.Y., Yoon, B.K., Lee, Y.J. and Chen, S.N. (2006). Aqueous extract of Schizandra chinensis fruit causes endothelium-dependent and -independent relaxation of isolated rat thoracic aorta. Phytomedicine, 13, 651-657.   DOI   ScienceOn
7 Shin, T.Y. (2003). Inhibition of immunologic and nonimmunologic stimulation-mediated anaphylactic reactions by the aqueous extract of Mentha arvensis. Immunopharmacol. Immunotoxicol., 25, 273-283.
8 Sun, H.X. and Pan, H.J. (2006). Immunological adjuvant effect of Glycyrrhiza uralensis saponins on the immune responses to ovalbumin in mice. Vaccine, 24, 1914-1920.   DOI   ScienceOn
9 Yamaguchi, C., Fujita, S., Obara, T. and Ueda, T. (1983). Effects of room temperature on reproduction, body weight and organ weights, food and water intakes, and hematology in mice. Exp. Anim., 32, 1-11.   DOI
10 Dourish, C.T. (1987). Effects of drugs on spontaneous motor activity in Experimental Psychopharmacology (Greenshaw, A.J. and Dourish, C.T. Eds.). Humana Press, Clifton, pp. 325-334.
11 Ghayur, M.N., Gilani, A.H. and Janssen, L.J. (2008). Ginger attenuates acetylcholine-induced contraction and Ca2+ signalling in murine airway smooth muscle cells. Can. J. Physiol. Pharmacol., 86, 264-271.   DOI
12 Irwin, S. (1968). Comprehensive observational assessment: Ia. A systemic, quantitative procedure for assessing the behavioral and physiological state of the mouse. Psychopharmacology, 13, 222-257.   DOI
13 Kim, S.S., Lee, S.C., Shin, H.D., Shin, M.K., Kim, J.H. and Song, H.J. (2004). Studies on allergy asthma effect of Radix Platicodi. Kor. J. Herbology, 19, 61-70.   과학기술학회마을
14 Korea Food and Drug Administration. Testing Guidelines for Safety Evaluation of Drugs (Notification No. 2005-60, issued by the Korea Food and Drug Administration on October 21, 2005), 2005.
15 Lee, H.S., Lee I.G. and Ku S.K. (2006). Single oral dose toxicity study of water extracts of Picrorrhiza Rhizoma in mice. J. Toxicol. Pub. Health, 22, 117-126.   과학기술학회마을
16 Lee, J.E., Kim, H.J., Choi, E.K., Chai, H.Y., Yun, Y.W., Kim, D.J., Nam, S.Y., Lee, B.J., Ahn, B.W., Kang, H.G. and Kim, Y.B. (2003). Four-week repeated-dose toxicity study on Pinellia Extract. Korean J. Lab. Anim. Sci., 19, 127-141.   과학기술학회마을
17 Lee, J.H, Yang, K.J., Shin, H.D., Park, B.R., Son, C.W., Jang, H.J., Park, D.C., Lee, H.S. amd Ku, S.K. (2005). Single subcutaneous dose toxicity of Polycan${\circledR}$, a $\beta$-glucan originated from Aureobasidium in mice. Lab. Anim. Res., 21, 299-305.   과학기술학회마을
18 Aimbire, F., Penna, S.C., Rodrigues, M., Rodrigues, K.C., Lopes- Martins, R.A. and Sertie, J.A. (2007). Effect of hydroalcoholic extract of Zingiber officinalis rhizomes on LPS-induced rat airway hyperreactivity and lung inflammation. Prostaglandins Leukot Essent Fatty Acids, 77, 129-138.   DOI   ScienceOn
19 Back, Y.D., Lee, H.S. and Ku, S.K. (2008). Effects of DHU001, a mixed herbal formula on acute inflammation in mice. Toxicol. Res., 24, 189-194.   과학기술학회마을   DOI   ScienceOn
20 Banks, W.J. (1986). Female reproductive system in Applied veterinary histology (Banks, W.J. Ed.). Williams & Wilkins, Baltimore, pp. 330-347, 506-526.