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http://dx.doi.org/10.5487/TR.2010.26.1.015

Identification of Differentially Expressed Genes in Human Mesenchymal Stem Cell-Derived Neurons  

Heo, Ji-Hye (Department of Biomedical Science, College of Health Sciences, Korea University)
Cho, Kyung-Jin (Department of Biomedical Science, College of Health Sciences, Korea University)
Choi, Dal-Woong (Department of Environmental Health, College of Health Sciences, Korea University)
Kim, Suhng-Wook (Department of Biomedical Science, College of Health Sciences, Korea University)
Publication Information
Toxicological Research / v.26, no.1, 2010 , pp. 15-19 More about this Journal
Abstract
Mesenchymal stem cells (MSCs) have greater potential for immediate clinical and toxicological applications, due to their ability to self-renew, proliferate, and differentiate into a variety of cell types. To identify novel candidate genes that were specifically expressed during transdifferentiation of human MSCs to neuronal cells, we performed a differential expression analysis with random priming approach using annealing control primer-based differential display reverse transcription-polymerase chain reaction approach. We identified genes for acyl-CoA thioesterase, tissue inhibitor of metalloproteinases-1, brain glycogen phosphorylase, ubiquitin C-terminal hydrolase and aldehyde reductase were up-regualted, whereas genes for transgelin and heparan sulfate proteoglycan were down-regulated in MSC-derived neurons. These differentially expressed genes may have potential role in regulation of neurogenesis. This study could be applied to environmental toxicology in the field of testing the toxicity of a chemical or a physical agent.
Keywords
Mesenchymal stem cells; Neuron; Differentially expressed genes;
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